IR@PKUHSC  > 北京大学基础医学院  > 病理学系
学科主题基础医学
Neural differentiation arrest in embryonal carcinoma cells with forced expression of EWS-FLI1
Yang, Yu1; Zhang, Lanjing2; Wei, Yanyu1; Wang, Hua1; Fukuma, Mariko3; Xu, Hao4; Xiong, Wei5; Zheng, Jie1
关键词Ewing&prime s sarcoma/primitive neuroectodermal tumor (EWS/PNET) EWS-FLI1 P19 cells neural differentiation
刊名JOURNAL OF NEURO-ONCOLOGY
2008-11-01
DOI10.1007/s11060-008-9646-x
90期:2页:141-150
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Clinical Neurology
研究领域[WOS]Oncology ; Neurosciences & Neurology
关键词[WOS]DOWN-REGULATION ; RETINOIC ACID ; EWING SARCOMA ; IN-VIVO ; GENE ; PROTEIN ; FUSION ; TRANSLOCATION ; TUMORS ; INHIBITION
英文摘要

Ewing′s sarcoma/primitive neuroectodermal tumor (EWS/PNET) has a characteristic chimeric oncogene EWS-FLI1, which results from chromosomal translocation t (11; 22), that is believed to initiate tumorigenesis of EWS/PNET. However, the specific details of EWS/PNET oncogenesis and exact role of EWS-FLI1 remain largely unknown. In this study we explored the role of EWS-FLI1 in tumor differentiation using an embryonal carcinoma cell line P19 as a model, with forced expression of EWS-FLI1 in these cells. EWS-FLI1 has been reported to promote neural differentiation in fibroblasts, mesenchymal stem cells and rhabdomyosarcoma cells. We show forced expression of EWS-FLI1 causes absence of retinoic acid-induced neural morphology, and decreases expression of neural-specific proteins MAPT and NCAM. Critical transcriptional factors for neural differentiation and stem cells are also altered in the presence of EWS-FLI1, including decreases in levels of Oct-3 and Pax-6, and an increase in the level of Id2, which is a target of EWS-FLI1. Increased proliferation and decreased apoptotic rates are also observed in P19 cells with forced expression of EWS-FLI1. Our results raise the possibility that arrest of neural differentiation by forced expression of EWS-FLI1 as observed in this study may result from dysregulation of the cell cycle and cell proliferation. Taken together, our results demonstrate that the modulation of neural differentiation in P19 cells which have a stem cell-like pluripotency in vitro can provide a novel model system to study the neural differentiation effects of EWS-FLI1 tumorigenesis of EWS/PNET.

语种英语
WOS记录号WOS:000259672100003
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58981
专题北京大学基础医学院_病理学系
作者单位1.Mt Sinai Sch Med, Dept Pathol, New York, NY 10029 USA
2.Peking Univ, Dept Pathol, Hlth Sci Ctr, Beijing 100083, Peoples R China
3.Keio Univ, Sch Med, Dept Pathol, Shinjuku Ku, Tokyo 1608582, Japan
4.Peking Univ, Dept Biochem & Mol Biol, Hlth Sci Ctr, Beijing 100083, Peoples R China
5.Univ British Columbia, Brain Res Ctr, Vancouver, BC V6T 2B5, Canada
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GB/T 7714
Yang, Yu,Zhang, Lanjing,Wei, Yanyu,et al. Neural differentiation arrest in embryonal carcinoma cells with forced expression of EWS-FLI1[J]. JOURNAL OF NEURO-ONCOLOGY,2008,90(2):141-150.
APA Yang, Yu.,Zhang, Lanjing.,Wei, Yanyu.,Wang, Hua.,Fukuma, Mariko.,...&Zheng, Jie.(2008).Neural differentiation arrest in embryonal carcinoma cells with forced expression of EWS-FLI1.JOURNAL OF NEURO-ONCOLOGY,90(2),141-150.
MLA Yang, Yu,et al."Neural differentiation arrest in embryonal carcinoma cells with forced expression of EWS-FLI1".JOURNAL OF NEURO-ONCOLOGY 90.2(2008):141-150.
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