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学科主题: 临床医学
题名:
Vaccination with Human Pluripotent Stem Cells Generates a Broad Spectrum of Immunological and Clinical Responses Against Colon Cancer
作者: Li, Yi1,4; Zeng, Hui2,3,5; Xu, Ren-He2,3; Liu, Bei1,2; Li, Zihai1,2
关键词: Embryonic stem cells ; Induced pluripotent stem cells ; Cancer vaccine ; Oncofetal antigen ; Myeloid-derived suppressor cells
刊名: STEM CELLS
发表日期: 2009-12-01
DOI: 10.1002/stem.234
卷: 27, 期:12, 页:3103-3111
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
关键词[WOS]: HEAT-SHOCK PROTEINS ; HUMAN SOMATIC-CELLS ; REGULATORY T-CELLS ; SUPPRESSOR-CELLS ; FETAL ANTIGENS ; DIRECTED DIFFERENTIATION ; CANCER/TESTIS ANTIGENS ; HUMAN BLASTOCYSTS ; DENDRITIC CELLS ; MURINE SARCOMAS
英文摘要:

The history of immunizing with embryonic materials to generate an antitumor immune response dates back to a century ago. The premise is that cancer cells share the expression of oncofetal antigens with embryonic materials and that the immune response against these antigens in the embryonic tissues is cross-protective against cancer. However, such a practice has never advanced beyond experimental animal settings, because of lack of uniformed source tissues and ethical challenges. With the availability of well-characterized human pluripotent stem cells, it is now possible to ask whether tumor protective immunity could indeed be elicited with stem cells. Herein, we investigated whether vaccination with defined human embryonic stem cells (hESCs) or induced pluripotent stem (iPS) cells was effective against a colon carcinoma. We discovered that vaccination of mice with hESC line H9 generated consistent cellular and humoral immune responses against CT26 colon carcinoma. Protection correlated strongly with the expansion of tumor-responsive and interferon-c-producing cells and the profound loss of CD11b(+)Gr-1(+) myeloid-derived suppressor cells in the spleen. No evidence of autoimmunity was observed. We also compared the immunogenicity against colon cancer between a hESC line CT2 and an iPS cell line TZ1 that were generated in the same stem cell facility. We found that the iPS cell line was inferior to the hESC line in conferring tumor protection, suggesting that there is heterogeneity of expression of oncofetal antigens by hESCs and iPS cells. We conclude that the hESC-based vaccine is a promising modality for immunotherapy of cancer. STEM CELLS 2009;27:3103-3111

语种: 英语
所属项目编号: 06SCA031 ; 06SCD02
项目资助者: Connecticut Stem Cell Research Grants Program
WOS记录号: WOS:000273569800023
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58984
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Univ Connecticut, Sch Med, Dept Immunol, Farmington, CT 06030 USA
2.Univ Connecticut, Sch Med, UConn Stem Cell Inst, Farmington, CT 06030 USA
3.Univ Connecticut, Sch Med, Dept Genet & Dev Biol, Farmington, CT 06030 USA
4.Peking Univ, Peoples Hosp, Dept Gynecol & Obstet, Beijing 100871, Peoples R China
5.Cent S Univ, Dept Hematol, Xiang Ya Hosp, Changsha, Hunan, Peoples R China

Recommended Citation:
Li, Yi,Zeng, Hui,Xu, Ren-He,et al. Vaccination with Human Pluripotent Stem Cells Generates a Broad Spectrum of Immunological and Clinical Responses Against Colon Cancer[J]. STEM CELLS,2009,27(12):3103-3111.
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