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学科主题临床医学
Early Expression of Tumor Necrosis Factor alpha and Nuclear Factor Kappa B After Descemet Stripping Endothelial Keratoplasty in Rabbits
Jin, Ying1; Chai, Li-jing1,2; Hong, Jing1
关键词Descemet stripping endothelial keratoplasty (DSEK) penetrating keratoplasty (PKP) tumor necrosis factor alpha (TNF-alpha) nuclear factor kappa B (NF kappa B) graft rejection
刊名CORNEA
2012-08-01
DOI10.1097/ICO.0b013e31823f8be0
31期:8页:913-919
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Ophthalmology
关键词[WOS]CORNEAL ALLOGRAFT-REJECTION ; GRAFT ; TRANSPLANT ; CYTOKINE ; TNF ; IDENTIFICATION ; INHIBITION ; KINASE ; CELLS ; EYES
英文摘要

Purpose: To investigate immune rejection after Descemet stripping endothelial keratoplasty (DSEK) compared with that after penetrating keratoplasty (PKP) in New Zealand rabbit eyes and to identify the relationship between tumor necrosis factor alpha (TNF-alpha) and nuclear factor kappa B (NF kappa B) and evaluate their involvement in graft rejection.

Methods: Sixty-nine New Zealand rabbits were divided into 3 groups: group A comprised the control group, which did not undergo either of the procedures; group B underwent DSEK; and group C underwent PKP. Twenty-three Chinese rabbits (46 eyes) were used as donors. The corneal transparency, graft placement, and anterior chamber were observed for 28 days. Blood serum, aqueous humor, and corneal samples were obtained on days 7, 14, 21, and 28 after the procedures. TNF-alpha levels were measured weekly by double-antibody enzyme-linked immunosorbent assay. NF kappa B in the cornea was checked by immunofluorescence.

Results: There were no graft rejections in group B. Group C showed a 50% rejection frequency with an increase in both the TNF-alpha concentrations and NF kappa B expression observed at 7 days and peak levels observed at 28 days after PKP. TNF-alpha concentrations did not differ significantly (P > 0.05) between groups B and A. NF kappa B expression was slightly increased at 7 days and returned to normal levels by day 14 after DSEK.

Conclusion: Rabbits with DSEK had a lower rejection rate than rabbits with PKP. NF kappa B and TNF-alpha may contribute to the early rejection of corneal allografts.

语种英语
所属项目编号30871315 ; 30670816
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000306325500013
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58985
专题北京大学第三临床医学院_眼科
作者单位1.Peking Univ, Dept Ophthalmol, Hosp 3, Beijing 100191, Peoples R China
2.Liaoyang Cent Hosp, Dept Ophthalmol, Liaoyang, Peoples R China
推荐引用方式
GB/T 7714
Jin, Ying,Chai, Li-jing,Hong, Jing. Early Expression of Tumor Necrosis Factor alpha and Nuclear Factor Kappa B After Descemet Stripping Endothelial Keratoplasty in Rabbits[J]. CORNEA,2012,31(8):913-919.
APA Jin, Ying,Chai, Li-jing,&Hong, Jing.(2012).Early Expression of Tumor Necrosis Factor alpha and Nuclear Factor Kappa B After Descemet Stripping Endothelial Keratoplasty in Rabbits.CORNEA,31(8),913-919.
MLA Jin, Ying,et al."Early Expression of Tumor Necrosis Factor alpha and Nuclear Factor Kappa B After Descemet Stripping Endothelial Keratoplasty in Rabbits".CORNEA 31.8(2012):913-919.
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