北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 期刊论文
学科主题: 临床医学
题名:
The FVIIa-tissue factor complex induces the expression of MMP7 in LOVO cells in vitro
作者: Zhang, Jian-Quan; Wan, Yuan-Lian; Liu, Yu-Cun; Wang, Xin; Tang, Jian-Qiang; Wu, Tao; Zhu, Jing; Pan, Yi-Sheng
关键词: MMP7 ; colon cancer ; FVIIa ; tissue factor ; promoter
刊名: INTERNATIONAL JOURNAL OF COLORECTAL DISEASE
发表日期: 2008-10-01
DOI: 10.1007/s00384-008-0496-y
卷: 23, 期:10, 页:971-978
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology ; Surgery
研究领域[WOS]: Gastroenterology & Hepatology ; Surgery
关键词[WOS]: CANINE KIDNEY-CELLS ; FACTOR VIIA ; MATRIX METALLOPROTEINASES ; COLORECTAL-CANCER ; GENE-EXPRESSION ; UP-REGULATION ; BINDING ; COAGULATION ; METASTASIS ; ACTIVATION
英文摘要:

Background and aims The extracellular interactions of plasma clotting factor VIIa (FVIIa) with tissue factor (TF) on the cell surface trigger intracellular signaling events involved in multiple physiological processes. TF expression is related to the metastatic potential of tumor cells and is a significant risk factor in the development of hepatic metastases in patients with colorectal cancer. At present, it is unclear how the interaction between TF and FVIIa influences the development of metastasis in colon cancer.

Materials and methods We used a stable LOVO cell line derived from colorectal adenocarcinoma for our model Western blot analysis, Northern blot analysis, polymerase chain reaction, and RNA inference (RNAi), and the Dual-Luciferase Reporter Assay System technology were utilized to determine if MMP7 can be up-regulated by the VIIa/TF complex.

Results Northern blot analysis confirmed that the plasma clotting factor FVIIa/TF complex resulted in a marked increase in MMP7 expression in a time- and dose-dependent manner via the p38 pathway in vitro. The proximal promoter of the human MMP7 gene was cloned into a luciferase reporter construction (MMP7.luc1592). Upon treatment with FVIIa, reporter activity in LOVO cells was increased by 2.5-fold. TF RNAi almost completely abolished FVIIa-mediated MMP7.luc induction. Deletion constructs from MMP7.luc1592 further defined an active promoter region.

Interpretation Taken together, these data provide evidence that expression of MMP7 in colon cancer may be regulated by FVIIa and TF at the transcriptional level. MMP7 may act as a downstream mediator of FVIIa/TF signal transduction to facilitate the development of metastasis in colon cancer.

语种: 英语
所属项目编号: 30471683 ; 02003 ; 985
项目资助者: National Nature and Science Foundation of China ; Key Project of Chinese Ministry of Education ; Foundation of Peking University
WOS记录号: WOS:000258884200008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58990
Appears in Collections:北京大学第一临床医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Hosp 1, Lab Colon Canc, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, Jian-Quan,Wan, Yuan-Lian,Liu, Yu-Cun,et al. The FVIIa-tissue factor complex induces the expression of MMP7 in LOVO cells in vitro[J]. INTERNATIONAL JOURNAL OF COLORECTAL DISEASE,2008,23(10):971-978.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Jian-Quan]'s Articles
[Wan, Yuan-Lian]'s Articles
[Liu, Yu-Cun]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Jian-Quan]‘s Articles
[Wan, Yuan-Lian]‘s Articles
[Liu, Yu-Cun]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace