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The FVIIa-tissue factor complex induces the expression of MMP7 in LOVO cells in vitro
Zhang, Jian-Quan; Wan, Yuan-Lian; Liu, Yu-Cun; Wang, Xin; Tang, Jian-Qiang; Wu, Tao; Zhu, Jing; Pan, Yi-Sheng
关键词MMP7 colon cancer FVIIa tissue factor promoter
刊名INTERNATIONAL JOURNAL OF COLORECTAL DISEASE
2008-10-01
DOI10.1007/s00384-008-0496-y
23期:10页:971-978
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology ; Surgery
研究领域[WOS]Gastroenterology & Hepatology ; Surgery
关键词[WOS]CANINE KIDNEY-CELLS ; FACTOR VIIA ; MATRIX METALLOPROTEINASES ; COLORECTAL-CANCER ; GENE-EXPRESSION ; UP-REGULATION ; BINDING ; COAGULATION ; METASTASIS ; ACTIVATION
英文摘要

Background and aims The extracellular interactions of plasma clotting factor VIIa (FVIIa) with tissue factor (TF) on the cell surface trigger intracellular signaling events involved in multiple physiological processes. TF expression is related to the metastatic potential of tumor cells and is a significant risk factor in the development of hepatic metastases in patients with colorectal cancer. At present, it is unclear how the interaction between TF and FVIIa influences the development of metastasis in colon cancer.

Materials and methods We used a stable LOVO cell line derived from colorectal adenocarcinoma for our model Western blot analysis, Northern blot analysis, polymerase chain reaction, and RNA inference (RNAi), and the Dual-Luciferase Reporter Assay System technology were utilized to determine if MMP7 can be up-regulated by the VIIa/TF complex.

Results Northern blot analysis confirmed that the plasma clotting factor FVIIa/TF complex resulted in a marked increase in MMP7 expression in a time- and dose-dependent manner via the p38 pathway in vitro. The proximal promoter of the human MMP7 gene was cloned into a luciferase reporter construction (MMP7.luc1592). Upon treatment with FVIIa, reporter activity in LOVO cells was increased by 2.5-fold. TF RNAi almost completely abolished FVIIa-mediated MMP7.luc induction. Deletion constructs from MMP7.luc1592 further defined an active promoter region.

Interpretation Taken together, these data provide evidence that expression of MMP7 in colon cancer may be regulated by FVIIa and TF at the transcriptional level. MMP7 may act as a downstream mediator of FVIIa/TF signal transduction to facilitate the development of metastasis in colon cancer.

语种英语
WOS记录号WOS:000258884200008
项目编号30471683 ; 02003 ; 985
资助机构National Nature and Science Foundation of China ; Key Project of Chinese Ministry of Education ; Foundation of Peking University
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58990
专题北京大学第一临床医学院
北京大学第一临床医学院_普通外科
作者单位Peking Univ, Hosp 1, Lab Colon Canc, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Jian-Quan,Wan, Yuan-Lian,Liu, Yu-Cun,et al. The FVIIa-tissue factor complex induces the expression of MMP7 in LOVO cells in vitro[J]. INTERNATIONAL JOURNAL OF COLORECTAL DISEASE,2008,23(10):971-978.
APA Zhang, Jian-Quan.,Wan, Yuan-Lian.,Liu, Yu-Cun.,Wang, Xin.,Tang, Jian-Qiang.,...&Pan, Yi-Sheng.(2008).The FVIIa-tissue factor complex induces the expression of MMP7 in LOVO cells in vitro.INTERNATIONAL JOURNAL OF COLORECTAL DISEASE,23(10),971-978.
MLA Zhang, Jian-Quan,et al."The FVIIa-tissue factor complex induces the expression of MMP7 in LOVO cells in vitro".INTERNATIONAL JOURNAL OF COLORECTAL DISEASE 23.10(2008):971-978.
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