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学科主题: 基础医学
题名:
Generation of Human-Induced Pluripotent Stem Cells from Gut Mesentery-Derived Cells by Ectopic Expression of OCT4/SOX2/NANOG
作者: Li, Yang2,3; Zhao, Hongxi4; Lan, Feng2,3,5; Lee, Andrew5; Chen, Liu2,3; Lin, Changsheng2,3; Yao, Yuanqing4; Li, Lingsong1,2,3
刊名: CELLULAR REPROGRAMMING
发表日期: 2010-06-01
DOI: 10.1089/cell.2009.0103
卷: 12, 期:3, 页:237-247
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]: HUMAN SOMATIC-CELLS ; HUMAN CORD BLOOD ; HUMAN FIBROBLASTS ; DEFINED FACTORS ; ES CELLS ; MOUSE ; NANOG ; OCT4 ; INDUCTION ; CLONING
英文摘要:

Induced pluripotent stem (iPS) cells have been generated from human somatic cells by ectopic expression of defined transcription factors. Application of this approach in human cells may have enormous potential to generate patient-specific pluripotent stem cells. However, traditional methods of reprogramming in human somatic cells involve the use of oncogenes c-MYC and KLF4, which are not applicable to clinical translation. In the present study, we investigated whether human fetal gut mesentery-derived cells (hGMDCs) could be successfully reprogrammed into induced pluripotent stem (iPS) cells by OCT4, SOX2, and NANOG alone. We used lentiviruses to express OCT4, SOX2, NANOG, in hGMDCs, then generated iPS cells that were identified by morphology, presence of pluripotency markers, global gene expression profile, DNA methylation status, capacity to form embryoid bodies (EBs), and terotoma formation. iPS cells resulting from hGMDCs were similar to human embryonic stem (ES) cells in morphology, proliferation, surface markers, gene expression, and epigenetic status of pluripotent cell-specific genes. Furthermore, these cells were able to differentiate into cell types of all three germ layers both in vitro and in vivo, as shown by EB and teratoma formation assays. DNA fingerprinting showed that the human iPS cells were derived from the donor cells, and are not a result of contamination. Our results provide proof that hGMDCs can be reprogrammed into pluripotent cells by ectopic expression of three factors (OCT4, SOX2, and NANOG) without the use of oncogenes c-MYC and KLF4.

语种: 英语
所属项目编号: 2006CB943603 ; 2006CB503905 ; 2006AA02A114 ; D0750701350704
项目资助者: Chinese National 973 Project ; Chinese National 863 Project ; Natural Science foundation of the Beijing Ministry of Science and Technology
WOS记录号: WOS:000279402900002
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59002
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.China Natl Ctr Int Res, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100191, Peoples R China
4.Fourth Mil Med Univ, Tangdu Hosp, Dept Genecol & Obstet, Xian 710038, Peoples R China
5.Stanford Univ, Sch Med, Dept Med, Div Cardiol, Stanford, CA 94305 USA

Recommended Citation:
Li, Yang,Zhao, Hongxi,Lan, Feng,et al. Generation of Human-Induced Pluripotent Stem Cells from Gut Mesentery-Derived Cells by Ectopic Expression of OCT4/SOX2/NANOG[J]. CELLULAR REPROGRAMMING,2010,12(3):237-247.
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