|Suppression of the Epidermal Growth Factor Receptor Inhibits Epithelial-Mesenchymal Transition in Human Pancreatic Cancer PANC-1 Cells|
|Chang, Zhi-Gang1,2; Wei, Jun-Min2; Qin, Chang-Fu1; Hao, Kun1; Tian, Xiao-Dong1; Xie, Kun1; Xie, Xue-Hai1; Yang, Yin-Mo1|
|关键词||Pancreatic cancer Epidermal growth factor receptor Epithelial-mesenchymal transition E-cadherin|
|刊名||DIGESTIVE DISEASES AND SCIENCES|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||THERAPEUTIC TARGETS ; CARCINOMA ; METASTASIS ; SNAIL ; GEMCITABINE ; EXPRESSION ; CHALLENGES ; RESISTANCE ; ADHESION ; IMPACT|
Aberrant expression of epidermal growth factor receptor (EGFR) has been detected in pancreatic cancer; however, the mechanisms of EGFR in inducing pancreatic cancer development have not been adequately elucidated. The objective of this study was to determine the role of EGFR in mediating epithelial-mesenchymal transition (EMT) in pancreatic cancer cells.
Pancreatic cancer cell line PANC-1 was transfected with small interfering RNA of EGFR by use of a lentiviral expression vector to establish an EGFR-knockdown cell line (si-PANC-1). PANC-1 cells transfected with lentiviral vector expressing negative control sequence were used as negative control (NC-PANC-1). Scratch assay and transwell study were used to analyze cell migration and invasion. Real-time PCR and Western blotting were used to detect the expression of EMT markers E-cadherin, N-cadherin, vimentin, and fibronectin and transcription factors snail, slug, twist1, and sip1 in PANC-1, NC-PANC-1, and si-PANC-1 cells. Immunofluorescent staining with these antibodies and confocal microscopy were used to observe their cellular location and morphologic changes.
After RNA interference of EGFR, the migration and invasion ability of si-PANC-1 cells decreased significantly. The expression of epithelial phenotype marker E-cadherin increased and the expression of mesenchymal phenotype markers N-cadherin, vimentin, and fibronectin decreased, indicating reversion of EMT. We also observed intracellular translocation of E-cadherin. Expression of transcription factors snail and slug in si-PANC-1 cells decreased significantly.
Suppression of EGFR expression can significantly inhibit EMT of pancreatic cancer PANC-1 cells. The mechanism may be related with the down-regulation of the expression of transcription factors snail and slug.
|资助机构||National Basic Research Program Grant ; National Natural Science Foundation, People&prime ; s Republic of China|
|作者单位||1.Beijing Hosp, Minist Hlth, Dept Gen Surg, Beijing 100730, Peoples R China|
2.Peking Univ, Hosp 1, Dept Gen Surg, Beijing 100034, Peoples R China
|Chang, Zhi-Gang,Wei, Jun-Min,Qin, Chang-Fu,et al. Suppression of the Epidermal Growth Factor Receptor Inhibits Epithelial-Mesenchymal Transition in Human Pancreatic Cancer PANC-1 Cells[J]. DIGESTIVE DISEASES AND SCIENCES,2012,57(5):1181-1189.|
|APA||Chang, Zhi-Gang.,Wei, Jun-Min.,Qin, Chang-Fu.,Hao, Kun.,Tian, Xiao-Dong.,...&Yang, Yin-Mo.(2012).Suppression of the Epidermal Growth Factor Receptor Inhibits Epithelial-Mesenchymal Transition in Human Pancreatic Cancer PANC-1 Cells.DIGESTIVE DISEASES AND SCIENCES,57(5),1181-1189.|
|MLA||Chang, Zhi-Gang,et al."Suppression of the Epidermal Growth Factor Receptor Inhibits Epithelial-Mesenchymal Transition in Human Pancreatic Cancer PANC-1 Cells".DIGESTIVE DISEASES AND SCIENCES 57.5(2012):1181-1189.|