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学科主题: 临床医学
题名:
Suppression of the Epidermal Growth Factor Receptor Inhibits Epithelial-Mesenchymal Transition in Human Pancreatic Cancer PANC-1 Cells
作者: Chang, Zhi-Gang1,2; Wei, Jun-Min2; Qin, Chang-Fu1; Hao, Kun1; Tian, Xiao-Dong1; Xie, Kun1; Xie, Xue-Hai1; Yang, Yin-Mo1
关键词: Pancreatic cancer ; Epidermal growth factor receptor ; Epithelial-mesenchymal transition E-cadherin
刊名: DIGESTIVE DISEASES AND SCIENCES
发表日期: 2012-05-01
DOI: 10.1007/s10620-012-2036-4
卷: 57, 期:5, 页:1181-1189
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: THERAPEUTIC TARGETS ; CARCINOMA ; METASTASIS ; SNAIL ; GEMCITABINE ; EXPRESSION ; CHALLENGES ; RESISTANCE ; ADHESION ; IMPACT
英文摘要:

Aberrant expression of epidermal growth factor receptor (EGFR) has been detected in pancreatic cancer; however, the mechanisms of EGFR in inducing pancreatic cancer development have not been adequately elucidated. The objective of this study was to determine the role of EGFR in mediating epithelial-mesenchymal transition (EMT) in pancreatic cancer cells.

Pancreatic cancer cell line PANC-1 was transfected with small interfering RNA of EGFR by use of a lentiviral expression vector to establish an EGFR-knockdown cell line (si-PANC-1). PANC-1 cells transfected with lentiviral vector expressing negative control sequence were used as negative control (NC-PANC-1). Scratch assay and transwell study were used to analyze cell migration and invasion. Real-time PCR and Western blotting were used to detect the expression of EMT markers E-cadherin, N-cadherin, vimentin, and fibronectin and transcription factors snail, slug, twist1, and sip1 in PANC-1, NC-PANC-1, and si-PANC-1 cells. Immunofluorescent staining with these antibodies and confocal microscopy were used to observe their cellular location and morphologic changes.

After RNA interference of EGFR, the migration and invasion ability of si-PANC-1 cells decreased significantly. The expression of epithelial phenotype marker E-cadherin increased and the expression of mesenchymal phenotype markers N-cadherin, vimentin, and fibronectin decreased, indicating reversion of EMT. We also observed intracellular translocation of E-cadherin. Expression of transcription factors snail and slug in si-PANC-1 cells decreased significantly.

Suppression of EGFR expression can significantly inhibit EMT of pancreatic cancer PANC-1 cells. The mechanism may be related with the down-regulation of the expression of transcription factors snail and slug.

语种: 英语
所属项目编号: 30972897
项目资助者: National Basic Research Program Grant ; National Natural Science Foundation, People&prime ; s Republic of China
WOS记录号: WOS:000303385100010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59029
Appears in Collections:北京大学第一临床医学院_普通外科_期刊论文

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作者单位: 1.Beijing Hosp, Minist Hlth, Dept Gen Surg, Beijing 100730, Peoples R China
2.Peking Univ, Hosp 1, Dept Gen Surg, Beijing 100034, Peoples R China

Recommended Citation:
Chang, Zhi-Gang,Wei, Jun-Min,Qin, Chang-Fu,et al. Suppression of the Epidermal Growth Factor Receptor Inhibits Epithelial-Mesenchymal Transition in Human Pancreatic Cancer PANC-1 Cells[J]. DIGESTIVE DISEASES AND SCIENCES,2012,57(5):1181-1189.
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