IR@PKUHSC  > 北京大学基础医学院  > 细胞生物学系
学科主题基础医学
Lidamycin induces neural differentiation of mouse embryonic carcinoma cells through down-regulation of transcription factor Oct4
Zhen, Hong-Ying1; He, Qi-Hua2; Li, Ying3; Zhou, Juan3; Yao, Cheng3; Liu, Yi-Nan1; Ma, Li-Jun4
关键词Lidamycin Embryonic carcinoma (EC) cell Neural differentiation Oct4 p21
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2012-04-27
DOI10.1016/j.bbrc.2012.03.105
421期:1页:44-50
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]STEM-CELLS ; RETINOIC ACID ; SOMATIC-CELLS ; SELF-RENEWAL ; CYCLE ARREST ; ES CELLS ; ACTIVATION ; EXPRESSION ; PATHWAY ; LINEAGE
英文摘要

Lidamycin is a potential anti-cancer drug, which is widely used in a variety of human cancer types. It has been reported that lidamycin inhibited mouse embryonic carcinoma (EC) cells growth through down-regulation of embryonic stem (ES) cell-like genes. In this study, whether 0.01 nM lidamycin induces neuronal differentiation of mouse EC cells was investigated. It was observed that lidamycin decreased transcription factor Oct4, and increased both p21 mRNA and protein expression in P19 EC cells. Furthermore, luciferase assay showed that lidamycin activated p21 promoter activity through suppression of Oct4, and Chromatin immunoprecipitation (ChIP) assay confirmed that binding of transcription factor Oct4 to the p21 promoter decreased in lidamycin-exposed cells. Knockdown of Oct4 resulted in neuron-like differentiation and up-regulation of p21 expression. In accordance, overexpression of Oct4 blocked neural differentiation and down-regulated p21 in lidamycin-treated P19 cells. Taken together, these results suggested that neuronal differentiation of EC cells induced by lidamycin was associated with the inhibition of Oct4 expression and the activation of p21 transcription. Our results have provided a novel mechanism, in which lidamycin led to cancer cell differentiation. (C) 2012 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000304501400009
项目编号2009ZX09301-010 ; 2009ZX09102-240 ; 7122098
资助机构Ministry of Science and Technology of the People&prime ; s Republic of China ; Beijing Natural Science Foundation
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59038
专题北京大学基础医学院_细胞生物学系
北京大学医学部管理机构_医学部
北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Med Anal Ctr, Beijing 100191, Peoples R China
3.Jinlin Univ, Hosp 1, Dept Oncol, Changchun, Peoples R China
4.Shanghai St Lukes Hosp, Dept Oncol, Shanghai 200050, Peoples R China
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Zhen, Hong-Ying,He, Qi-Hua,Li, Ying,et al. Lidamycin induces neural differentiation of mouse embryonic carcinoma cells through down-regulation of transcription factor Oct4[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2012,421(1):44-50.
APA Zhen, Hong-Ying.,He, Qi-Hua.,Li, Ying.,Zhou, Juan.,Yao, Cheng.,...&Ma, Li-Jun.(2012).Lidamycin induces neural differentiation of mouse embryonic carcinoma cells through down-regulation of transcription factor Oct4.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,421(1),44-50.
MLA Zhen, Hong-Ying,et al."Lidamycin induces neural differentiation of mouse embryonic carcinoma cells through down-regulation of transcription factor Oct4".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 421.1(2012):44-50.
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