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学科主题: 临床医学
题名:
Identification of a novel function of Id-1 in mediating the anticancer responses of SAMC, a water-soluble garlic derivative, in human bladder cancer cells
作者: Hu, Hao1,2; Zhang, Xiao Peng1; Wang, Yu Liang1; Chua, Chee Wai2; Luk, Sze Ue2; Wong, Yong Chuan2; Ling, Ming Tat2; Wang, Xiao Feng1; Xu, Ke Xin1
关键词: inhibitor of differentiation ; S-allylmercaptocysteine ; bladder cancer cells
刊名: MOLECULAR MEDICINE REPORTS
发表日期: 2011
DOI: 10.3892/mmr.2010.380
卷: 4, 期:1, 页:9-16
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental
研究领域[WOS]: Oncology ; Research & Experimental Medicine
关键词[WOS]: KINASE INHIBITOR P21 ; GROWTH-FACTOR-BETA ; PROSTATE-CANCER ; INDUCED APOPTOSIS ; CARCINOMA-CELLS ; CYCLE ARREST ; EXPRESSION ; INVASION ; PROLIFERATION ; PROTEINS
英文摘要:

Studies have shown that the expression of inhibitor of differentiation (Id-1) is increased in bladder cancer and is associated with drug resistance. S-allylmercaptocysteine (SAMC), a water-soluble component of garlic, is known to have a potent therapeutic effect on human cancer. The aim of this study was to investigate whether Id-1 expression mediates SAMC-induced cell death in bladder cancer cells. After generating stable Id-1-expressing and si-Id-1 transfectants in various bladder cancer cell lines, cell sensitivity to SAMC was compared by colony formation and MTT assays. The results indicated that Id-1 overexpression reduced the positive effect of SAMC on cell survival, while the inactivation of Id-1 increased cellular susceptibility to SAMC. Using DAPI staining, the apoptosis of bladder cancer cells induced by SAMC was shown to be negatively regulated by Id-1 expression. The expression of apoptosis-related proteins analyzed by Western blotting further supported the negative role of Id-1 in SAMC-induced apoptosis. Furthermore, by wound closure and type I collagen invasion assays, the inhibitory effect of SAMC on the invasion and migration of bladder cancer cells was found to be associated with the down-regulation of Id-1. Our results demonstrated that SAMC-induced apoptosis is associated with the Id-1 pathway, and that the inactivation of Id-1 enhances the ability of SAMC to inhibit the survival, invasion and migration of bladder cancer cells. These findings may lead to the development of novel therapeutic strategies for the treatment of bladder cancer.

语种: 英语
所属项目编号: RDB2008-30
项目资助者: Peking University People&prime ; s Hospital
WOS记录号: WOS:000286301100002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59042
Appears in Collections:北京大学第二临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ Peoples Hosp, Dept Urol, Peking Univ Hlth Sci Ctr, Beijing 100044, Peoples R China
2.Univ Hong Kong, Dept Anat, Fac Med, Canc Biol Grp, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Hu, Hao,Zhang, Xiao Peng,Wang, Yu Liang,et al. Identification of a novel function of Id-1 in mediating the anticancer responses of SAMC, a water-soluble garlic derivative, in human bladder cancer cells[J]. MOLECULAR MEDICINE REPORTS,2011,4(1):9-16.
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