IR@PKUHSC  > 北京大学第二临床医学院  > 泌尿外科
学科主题临床医学
Identification of a novel function of Id-1 in mediating the anticancer responses of SAMC, a water-soluble garlic derivative, in human bladder cancer cells
Hu, Hao1,2; Zhang, Xiao Peng1; Wang, Yu Liang1; Chua, Chee Wai2; Luk, Sze Ue2; Wong, Yong Chuan2; Ling, Ming Tat2; Wang, Xiao Feng1; Xu, Ke Xin1
关键词inhibitor of differentiation S-allylmercaptocysteine bladder cancer cells
刊名MOLECULAR MEDICINE REPORTS
2011
DOI10.3892/mmr.2010.380
4期:1页:9-16
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental
研究领域[WOS]Oncology ; Research & Experimental Medicine
关键词[WOS]KINASE INHIBITOR P21 ; GROWTH-FACTOR-BETA ; PROSTATE-CANCER ; INDUCED APOPTOSIS ; CARCINOMA-CELLS ; CYCLE ARREST ; EXPRESSION ; INVASION ; PROLIFERATION ; PROTEINS
英文摘要

Studies have shown that the expression of inhibitor of differentiation (Id-1) is increased in bladder cancer and is associated with drug resistance. S-allylmercaptocysteine (SAMC), a water-soluble component of garlic, is known to have a potent therapeutic effect on human cancer. The aim of this study was to investigate whether Id-1 expression mediates SAMC-induced cell death in bladder cancer cells. After generating stable Id-1-expressing and si-Id-1 transfectants in various bladder cancer cell lines, cell sensitivity to SAMC was compared by colony formation and MTT assays. The results indicated that Id-1 overexpression reduced the positive effect of SAMC on cell survival, while the inactivation of Id-1 increased cellular susceptibility to SAMC. Using DAPI staining, the apoptosis of bladder cancer cells induced by SAMC was shown to be negatively regulated by Id-1 expression. The expression of apoptosis-related proteins analyzed by Western blotting further supported the negative role of Id-1 in SAMC-induced apoptosis. Furthermore, by wound closure and type I collagen invasion assays, the inhibitory effect of SAMC on the invasion and migration of bladder cancer cells was found to be associated with the down-regulation of Id-1. Our results demonstrated that SAMC-induced apoptosis is associated with the Id-1 pathway, and that the inactivation of Id-1 enhances the ability of SAMC to inhibit the survival, invasion and migration of bladder cancer cells. These findings may lead to the development of novel therapeutic strategies for the treatment of bladder cancer.

语种英语
WOS记录号WOS:000286301100002
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59042
专题北京大学第二临床医学院_泌尿外科
作者单位1.Peking Univ Peoples Hosp, Dept Urol, Peking Univ Hlth Sci Ctr, Beijing 100044, Peoples R China
2.Univ Hong Kong, Dept Anat, Fac Med, Canc Biol Grp, Hong Kong, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714
Hu, Hao,Zhang, Xiao Peng,Wang, Yu Liang,et al. Identification of a novel function of Id-1 in mediating the anticancer responses of SAMC, a water-soluble garlic derivative, in human bladder cancer cells[J]. MOLECULAR MEDICINE REPORTS,2011,4(1):9-16.
APA Hu, Hao.,Zhang, Xiao Peng.,Wang, Yu Liang.,Chua, Chee Wai.,Luk, Sze Ue.,...&Xu, Ke Xin.(2011).Identification of a novel function of Id-1 in mediating the anticancer responses of SAMC, a water-soluble garlic derivative, in human bladder cancer cells.MOLECULAR MEDICINE REPORTS,4(1),9-16.
MLA Hu, Hao,et al."Identification of a novel function of Id-1 in mediating the anticancer responses of SAMC, a water-soluble garlic derivative, in human bladder cancer cells".MOLECULAR MEDICINE REPORTS 4.1(2011):9-16.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Hu, Hao]的文章
[Zhang, Xiao Peng]的文章
[Wang, Yu Liang]的文章
百度学术
百度学术中相似的文章
[Hu, Hao]的文章
[Zhang, Xiao Peng]的文章
[Wang, Yu Liang]的文章
必应学术
必应学术中相似的文章
[Hu, Hao]的文章
[Zhang, Xiao Peng]的文章
[Wang, Yu Liang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。