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学科主题: 药学
题名:
Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances
作者: Liu Xue-Hui; Lin Dong-Hai
关键词: NMR ; resonance overlapping ; amide-exchange-rate-edited ; SEA ; presat ; TROSY
刊名: CHINESE JOURNAL OF CHEMISTRY
发表日期: 2007-03-01
卷: 25, 期:3, 页:411-416
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: SOLVENT-EXPOSED AMIDES ; PROTEINS ; GRADIENT ; TROSY ; WATER
英文摘要:

This paper describes an amide-exchange-rate-edited (AERE) NMR method that can effectively alleviate the problem of resonance overlap for proteins and peptides. This method exploits the diversity of amide proton exchange rates and consists of two complementary experiments: (1) SEA (solvent exposed amide)-type NMR experiments to map exchangeable surface residues whose amides are not involved in hydrogen bonding, and (2) pre-sat-type NMR experiments to map solvent inaccessibly buried residues or nonexchangeable residues located in hydrogen-bonded secondary structures with properly controlled saturation transfer via amide proton exchanges with the solvent. This method separates overlapping resonances in a spectrum into two complementary spectra. The AERE-NMR method was demonstrated with a sample of N-15/C-13/H-2(70%) labeled ribosome-inactivating protein trichosanthin of 247 residues.

语种: 英语
WOS记录号: WOS:000244805800028
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59058
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Liu Xue-Hui,Lin Dong-Hai. Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances[J]. CHINESE JOURNAL OF CHEMISTRY,2007,25(3):411-416.
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