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Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances
Liu Xue-Hui; Lin Dong-Hai
关键词NMR resonance overlapping amide-exchange-rate-edited SEA presat TROSY
刊名CHINESE JOURNAL OF CHEMISTRY
2007-03-01
25期:3页:411-416
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]SOLVENT-EXPOSED AMIDES ; PROTEINS ; GRADIENT ; TROSY ; WATER
英文摘要

This paper describes an amide-exchange-rate-edited (AERE) NMR method that can effectively alleviate the problem of resonance overlap for proteins and peptides. This method exploits the diversity of amide proton exchange rates and consists of two complementary experiments: (1) SEA (solvent exposed amide)-type NMR experiments to map exchangeable surface residues whose amides are not involved in hydrogen bonding, and (2) pre-sat-type NMR experiments to map solvent inaccessibly buried residues or nonexchangeable residues located in hydrogen-bonded secondary structures with properly controlled saturation transfer via amide proton exchanges with the solvent. This method separates overlapping resonances in a spectrum into two complementary spectra. The AERE-NMR method was demonstrated with a sample of N-15/C-13/H-2(70%) labeled ribosome-inactivating protein trichosanthin of 247 residues.

语种英语
WOS记录号WOS:000244805800028
Citation statistics
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59058
Collection北京大学药学院
作者单位1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Liu Xue-Hui,Lin Dong-Hai. Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances[J]. CHINESE JOURNAL OF CHEMISTRY,2007,25(3):411-416.
APA Liu Xue-Hui,&Lin Dong-Hai.(2007).Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances.CHINESE JOURNAL OF CHEMISTRY,25(3),411-416.
MLA Liu Xue-Hui,et al."Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances".CHINESE JOURNAL OF CHEMISTRY 25.3(2007):411-416.
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