北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 药理学系  > 期刊论文
学科主题: 基础医学
题名:
The tumor suppressor gene RBM5 inhibits lung adenocarcinoma cell growth and induces apoptosis
作者: Shao, Chen2,3; Zhao, Lijing2; Wang, Ke1; Xu, Wei1; Zhang, Jie1; Yang, Baoxue2,4
关键词: RBM5 ; Lung adenocarcinoma ; Apoptosis ; A549 ; Xenograft mice model
刊名: WORLD JOURNAL OF SURGICAL ONCOLOGY
发表日期: 2012-08-06
DOI: 10.1186/1477-7819-10-160
卷: 10
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Surgery
研究领域[WOS]: Oncology ; Surgery
关键词[WOS]: CD95-MEDIATED APOPTOSIS ; HOMOZYGOUS DELETION ; CHROMOSOME 3P21.3 ; CANCER CELLS ; IN-VITRO ; REGION ; RNA ; EPIDEMIOLOGY ; COEXPRESSION ; METASTASIS
英文摘要:

Background: The loss of tumor suppressor gene (TSG) function is a critical step in the pathogenesis of human lung cancer. RBM5 (RNA-binding motif protein 5, also named H37/LUCA-15) gene from chromosome 3p21.3 demonstrated tumor suppressor activity. However, the role of RBM5 played in the occurrence and development of lung cancer is still not well understood.

Method: Paired non-tumor and tumor tissues were obtained from 30 adenocarcinomas. The expression of RBM5 mRNA and protein was examined by RT-PCR and Western blot. A549 cell line was used to determine the apoptotic function of RBM5 in vitro. A549 cells were transiently transfected with pcDNA3.1-RBM5. AnnexinV analysis was performed by flow cytometry. Expression of Bcl-2, cleaved caspase-3, caspase-9 and PAPP proteins in A549 lung cancer cells and the A549 xenograft BALB/c nude mice model was determined by Western blot. Tumor suppressor activity of RBM5 was also examined in the A549 xenograft model treated with pcDNA3.1-RBM5 plasmid carried by attenuated Salmonella typhi Ty21a.

Result: The expression of RBM5 mRNA and protein was decreased significantly in adenocarcinoma tissues compared to that in the non-tumor tissues. In addition, as compared to the vector control, a significant growth inhibition of A549 lung cancer cells was observed when transfected with pcDNA3.1-RBM5 as determined by cell proliferation assay. We also found that overexpression of RBM5 induced both early and late apoptosis in A549 cells using AnnexinV/PI staining as determined by flow cytometry. Furthermore, the expression of Bcl-2 protein was decreased, whereas the expression of cleaved caspase-3, caspase-9 and PARP proteins was significantly increased in the RBM5 transfected cells; similarly, expression of decreased Bcl-2 and increased cleaved caspase-3 proteins was also examined in the A549 xenograft model. More importantly, we showed that accumulative and stable overexpression of RBM5 in the A549 xenograft BALB/c nude mice model significantly inhibited the tumor growth rate in vivo as compared to that in the control.

Conclusion: Our study demonstrates that RBM5 can inhibit the growth of lung cancer cells and induce apoptosis both in vitro and in vivo, which suggests that RBM5 might be used as a potential biomarker or target for lung cancer diagnosis and chemotherapy. Moreover, we propose a novel animal model set up in BALB/c nude mice treated with attenuated Salmonella as a vector carrying plasmids to determine RBM5 function in vivo.

语种: 英语
所属项目编号: 81071919 ; 30971315 ; 201015169
项目资助者: National Natural Science Foundation of China ; Provincial Natural Science Foundation of Jilin
WOS记录号: WOS:000311540800001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59089
Appears in Collections:基础医学院_药理学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Jilin Univ, Affiliated Hosp 2, Dept Resp Med, Changchun 130041, Jilin, Peoples R China
2.Jilin Univ, Norman Bethune Coll Med, Dept Pathophysiol, Changchun 130021, Jilin, Peoples R China
3.Jilin Univ, China Japan Union Hosp, Dept Digest Med, Changchun 130033, Jilin, Peoples R China
4.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100191, Peoples R China

Recommended Citation:
Shao, Chen,Zhao, Lijing,Wang, Ke,et al. The tumor suppressor gene RBM5 inhibits lung adenocarcinoma cell growth and induces apoptosis[J]. WORLD JOURNAL OF SURGICAL ONCOLOGY,2012,10.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Shao, Chen]'s Articles
[Zhao, Lijing]'s Articles
[Wang, Ke]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Shao, Chen]‘s Articles
[Zhao, Lijing]‘s Articles
[Wang, Ke]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace