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学科主题: 药学
题名:
Potential of hyphenated ultra-high performance liquid chromatography-scheduled multiple reaction monitoring algorithm for large-scale quantitative analysis of traditional Chinese medicines
作者: Song, Qingqing1,2; Song, Yuelin1; Zhang, Na1,2; Li, Jun1; Jiang, Yong3; Zhang, Kerong4; Zhang, Qian1; Tu, Pengfei1
刊名: RSC ADVANCES
发表日期: 2015
DOI: 10.1039/c5ra09429a
卷: 5, 期:71, 页:57372-57382
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: TANDEM MASS-SPECTROMETRY ; TIME-OF-FLIGHT ; DIODE-ARRAY DETECTION ; ENANTIOSPECIFIC DETERMINATION ; DRUG-RESISTANCE ; HUMAN SERUM ; QUANTIFICATION ; CANCER ; PLASMA ; METABOLITES
英文摘要:

It is a great challenge to perform quality control for traditional Chinese medicines (TCMs) that contain a great number of constituents by holistically monitoring hydrophilic and hydrophobic substances. Theoretically, the relatively low scan rate of triple quadrupole (QqQ) equipment makes it quite difficult to meet the demands of reliable quantitation of the narrow peaks generated from ultra-high performance liquid chromatography (UHPLC). Scheduled multiple reaction monitoring (sMRM) algorithm offers the potential to simultaneously monitor numerous analytes without compromising data quality, in particular for co-eluting compounds, by automatically altering the dwell time to maintain the desired cycle time on a QqQ analytical platform. In the current study, UHPLC and sMRM were hyphenated to develop a practical and robust quantitative method for as many as 133 TCM-derived components, including polar and apolar compounds. Efficient separation was achieved on a core-shell-type column (Capcell core ADME column) with adamantylethyl functional groups to generate appropriate surface polarity along with hydrophobicity in comparison with RP-C-18 and HILIC columns. To verify the applicability of the developed UHPLC-sMRM method, a formula was simulated by mixing eight TCM raw materials that related to those 133 analytes. Moreover, enhanced product ion scans were triggered by sMRM to acquire MS2 spectra to enhance the confidence of peak assignment. Method validation results suggested the developed method to be accurate, precise, and reproducible. In comparison with conventional MRM, sMRM was proved to be advantageous in terms of sensitivity and precision, as well as the dependent MS2 spectral quality. Above all, our current study indicated that the integration of UHPLC and sMRM provides the potential to globally and simultaneously quantify the components in TCMs.

语种: 英语
所属项目编号: 81403073 ; NCET-13-0693 ; 81222051
项目资助者: National Science Fund of China ; Ministry of Industry and Information Technology of China ; Program for New Century Excellent Talents in University ; National Science Fund for Excellent Young Scholars
WOS记录号: WOS:000357805500015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59113
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.AB SCIEX, Applicat Support Ctr, Shanghai 200233, Peoples R China
2.Beijing Univ Chinese Med, Modern Res Ctr Tradit Chinese Med, Beijing 100029, Peoples R China
3.Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 100102, Peoples R China
4.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Song, Qingqing,Song, Yuelin,Zhang, Na,et al. Potential of hyphenated ultra-high performance liquid chromatography-scheduled multiple reaction monitoring algorithm for large-scale quantitative analysis of traditional Chinese medicines[J]. RSC ADVANCES,2015,5(71):57372-57382.
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