IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所
学科主题临床医学
In vivo and in vitro osteogenesis of stem cells induced by controlled release of drugs from microspherical scaffolds
Shi, Xuetao1,4; Ren, Li1,4; Tian, Ming2; Yu, Jiakuo2; Huang, Wei1,4; Du, Chang1,4; Wang, Dong-An3,4; Wang, Yingjun1
刊名JOURNAL OF MATERIALS CHEMISTRY
2010
DOI10.1039/c0jm00976h
20期:41页:9140-9148
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Physical ; Materials Science, Multidisciplinary
研究领域[WOS]Chemistry ; Materials Science
关键词[WOS]MARROW STROMAL CELLS ; BONE REPAIR ; TISSUE ; BISPHOSPHONATES ; HYDROXYAPATITE ; DEXAMETHASONE ; ALENDRONATE ; DIFFERENTIATION ; CARTILAGE ; ASCORBATE-2-PHOSPHATE
英文摘要

In this study, the in vivo and in vitro osteogenesis was successfully induced in situ by the controlled release of alendronate (AL) and dexamethasone (Dex) from the poly(lactic-co-glycolic acid) (PLGA)/hydroxyapatite (HA) sintering microsphere scaffold (SMS). This microspherically based controlled release system is constructed with two-levels of structure: AL attached HA particles and a Dex-PLGA matrix. Bone marrow mesenchymal stem cells (BMSCs) cultured on AL loaded PLGA/HA sintering microsphere scaffold (PLGA/HA-AL-SMS) and AL-Dex loaded PLGA/HA sintering microsphere scaffold (PLGA/HA-Com-SMS) displayed a strong osteogenic commitment locally in vitro. The osteogenic commitment of BMSCs on the scaffolds were verified by an alkaline phosphatase (ALP) activity assay, real time-PCR and immunohistochemistry analysis. The results demonstrated that BMSCs cultured on PLGA/HA-AL-SMS and PLGA/HA-Com-SMS exhibited superior osteogenic differentiation owing to significantly high gene expression of typical osteogenic genes-osteocalcin (OC), type I collagen, BMP-2, and also protein secretion of type I collagen. In addition, BMSCs laden with PLGA/HA-AL-SMS and PLGA/HA-Com-SMS were also implanted into the back subcutis of mice and bone defects surgically created on rabbit femurs. The histology and immunohistochemistry results indicated that the scaffolds promoted BMSCs osteogenic commitment and also the new bone formation in vivo. In conclusion, this controlled release system is promising for implanted biomedical devices to improve bone reparation and regeneration.

语种英语
WOS记录号WOS:000282871600024
项目编号2007U33046 ; 50572029 ; 305012 ; 50732003
资助机构China Scholarship Council (CSC) ; National Natural Science Foundation of China ; Ministry of Education ; National Natural Science of China
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59116
专题北京大学第三临床医学院_运动医学研究所
作者单位1.S China Univ Technol, Sch Mat Sci & Engn, Guangzhou 510641, Guangdong, Peoples R China
2.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100871, Peoples R China
3.Nanyang Technol Univ, Div Bioengn, Sch Chem & Biomed Engn, Singapore 637457, Singapore
4.S China Univ Technol, Key Lab Specially Funct Mat & Adv Mfg Technol, Minist Educ, Guangzhou 510641, Peoples R China
推荐引用方式
GB/T 7714
Shi, Xuetao,Ren, Li,Tian, Ming,et al. In vivo and in vitro osteogenesis of stem cells induced by controlled release of drugs from microspherical scaffolds[J]. JOURNAL OF MATERIALS CHEMISTRY,2010,20(41):9140-9148.
APA Shi, Xuetao.,Ren, Li.,Tian, Ming.,Yu, Jiakuo.,Huang, Wei.,...&Wang, Yingjun.(2010).In vivo and in vitro osteogenesis of stem cells induced by controlled release of drugs from microspherical scaffolds.JOURNAL OF MATERIALS CHEMISTRY,20(41),9140-9148.
MLA Shi, Xuetao,et al."In vivo and in vitro osteogenesis of stem cells induced by controlled release of drugs from microspherical scaffolds".JOURNAL OF MATERIALS CHEMISTRY 20.41(2010):9140-9148.
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