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学科主题: 药学
题名:
Identification of isoflavonoids in Radix Puerariae for quality control using on-line high performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometry coupled with post-column derivatization
作者: Niu, Yaru; Li, Hui; Dong, Jing2; Wang, Hong; Hashi, Yuki2; Chen, Shizhong1
关键词: HPLC-DAD-IT-TOF-MSn coupled with post-column derivatization (PCD) ; Radix Puerariae ; Isoflavonoids ; Quality evaluation
刊名: FOOD RESEARCH INTERNATIONAL
发表日期: 2012-10-01
DOI: 10.1016/j.foodres.2012.05.021
卷: 48, 期:2, 页:528-537
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Food Science & Technology
研究领域[WOS]: Food Science & Technology
关键词[WOS]: HPLC-UV-MS ; CAPILLARY-ELECTROPHORESIS ; ASSISTED EXTRACTION ; PHENOLIC-COMPOUNDS ; PLANT-EXTRACTS ; BREAST-CANCER ; ROOT EXTRACT ; KUDZU-ROOT ; LOBATA ; FLAVONOIDS
英文摘要:

Radix Puerariae (RP) is a botanical supplement widely used as a nutraceutical in functional foods. The developed method by coupling HPLC-DAD-IT-TOF-MSn with post-column derivatization (PCD) has been used for separation and identification of 22 isoflavones in RP. In addition, a total of 15 isoflavones were simultaneously determined in one single HPLC run with photodiode array detection (DAD), 8 of which were quantified in Radix Puerariae for the first time. Content determination results were used to comprehensively evaluate qualities of Radix Puerariae samples from different natural habitats in China. A YMC (TM) C-18 column (150 mm x 4.6 mm id.: 5 pm particle size) and gradient elution (acetonitrile and water with 0.02% (v/v) of formic acid) were used during the analysis. All calibration curves showed good linearity (r>0.9996) within the test ranges. The intra-day and inter-day stability for 15 analytes were less than 1.32% and 1.87%, respectively. The recoveries were between 95.1% and 103.5%. The established method was simple, feasible and practical, and could be applied to routine analysis of Radix Puerariae and its preparation. The research set a good example for the comprehensive quality control of Radix Puerariae. (C) 2012 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 2009ZX09308
项目资助者: national innovative medicines research and development project ; Shimadzu International Trading (Shanghai) Co. Limited
WOS记录号: WOS:000311185100025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59121
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.Peking Univ, Dept Nat Med, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Shimadzu Int Trading Shanghai, Beijing Off, Beijing 100020, Peoples R China

Recommended Citation:
Niu, Yaru,Li, Hui,Dong, Jing,et al. Identification of isoflavonoids in Radix Puerariae for quality control using on-line high performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometry coupled with post-column derivatization[J]. FOOD RESEARCH INTERNATIONAL,2012,48(2):528-537.
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