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Identification of isoflavonoids in Radix Puerariae for quality control using on-line high performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometry coupled with post-column derivatization
Niu, Yaru; Li, Hui; Dong, Jing2; Wang, Hong; Hashi, Yuki2; Chen, Shizhong1
关键词HPLC-DAD-IT-TOF-MSn coupled with post-column derivatization (PCD) Radix Puerariae Isoflavonoids Quality evaluation
刊名FOOD RESEARCH INTERNATIONAL
2012-10-01
DOI10.1016/j.foodres.2012.05.021
48期:2页:528-537
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Food Science & Technology
研究领域[WOS]Food Science & Technology
关键词[WOS]HPLC-UV-MS ; CAPILLARY-ELECTROPHORESIS ; ASSISTED EXTRACTION ; PHENOLIC-COMPOUNDS ; PLANT-EXTRACTS ; BREAST-CANCER ; ROOT EXTRACT ; KUDZU-ROOT ; LOBATA ; FLAVONOIDS
英文摘要

Radix Puerariae (RP) is a botanical supplement widely used as a nutraceutical in functional foods. The developed method by coupling HPLC-DAD-IT-TOF-MSn with post-column derivatization (PCD) has been used for separation and identification of 22 isoflavones in RP. In addition, a total of 15 isoflavones were simultaneously determined in one single HPLC run with photodiode array detection (DAD), 8 of which were quantified in Radix Puerariae for the first time. Content determination results were used to comprehensively evaluate qualities of Radix Puerariae samples from different natural habitats in China. A YMC (TM) C-18 column (150 mm x 4.6 mm id.: 5 pm particle size) and gradient elution (acetonitrile and water with 0.02% (v/v) of formic acid) were used during the analysis. All calibration curves showed good linearity (r>0.9996) within the test ranges. The intra-day and inter-day stability for 15 analytes were less than 1.32% and 1.87%, respectively. The recoveries were between 95.1% and 103.5%. The established method was simple, feasible and practical, and could be applied to routine analysis of Radix Puerariae and its preparation. The research set a good example for the comprehensive quality control of Radix Puerariae. (C) 2012 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000311185100025
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59121
专题北京大学药学院_天然药物学系
作者单位1.Peking Univ, Dept Nat Med, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Shimadzu Int Trading Shanghai, Beijing Off, Beijing 100020, Peoples R China
推荐引用方式
GB/T 7714
Niu, Yaru,Li, Hui,Dong, Jing,et al. Identification of isoflavonoids in Radix Puerariae for quality control using on-line high performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometry coupled with post-column derivatization[J]. FOOD RESEARCH INTERNATIONAL,2012,48(2):528-537.
APA Niu, Yaru,Li, Hui,Dong, Jing,Wang, Hong,Hashi, Yuki,&Chen, Shizhong.(2012).Identification of isoflavonoids in Radix Puerariae for quality control using on-line high performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometry coupled with post-column derivatization.FOOD RESEARCH INTERNATIONAL,48(2),528-537.
MLA Niu, Yaru,et al."Identification of isoflavonoids in Radix Puerariae for quality control using on-line high performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometry coupled with post-column derivatization".FOOD RESEARCH INTERNATIONAL 48.2(2012):528-537.
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