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学科主题: 临床医学
题名:
The signal transduction pathway in the proliferation of airway smooth muscle cells induced by urotensin II
作者: Chen, YH; Zhao, MW; Yao, WZ; Pang, YZ; Tang, CS
关键词: urotensin II ; airway smooth muscle cells ; protein kinase C ; mitogen-activated protein kinase ; calcineurin
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2004
卷: 117, 期:1, 页:37-41
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: CALCINEURIN
英文摘要:

Background Human urotensin II (UII) is the most potent mammalian vasoconstrictor identified so far. Our previous study showed that UII is a potent mitogen of airway smooth muscle cells (ASMC) inducing ASMC proliferation in a dose-dependent manner. The signal transduction pathway of UII mitogenic effect remains to be clarified. This study was conducted to investigate the signal transduction pathway in the proliferation of ASMC induced by UII.

Methods In primary cultures of rat ASMCs, activities of protein kinase C ( PKC), mitogen-activated protein kinase (MAPK) and calcineurin (CaN) induced by UII were measured. The effect of CaN on PKC and MAPK was studied by adding cyclosporin A (CsA), a specific inhibitor of CaN. Using H, and PD98059, inhibitors of PKC and MAPK, respectively, to study the effect of PKC and MAPK on CaN. The cytosolic free calcium concentration induced by UII was measured using Fura-2/AM.

Results UII 10(-7) mol/L stimulated ASMC PKC and MAPK activities by 44% and 24% (P < 0.01) respectively, after incubating for 20 minutes. It increased CaN activity in a time-dependent manner, being 1.68 times as that of control for 24 hours (P < 0.01). It promoted the cytosolic free calcium concentration increase of 18% (P < 0.01). CsA 10(-6) mol/L and H-7 50 mumol/L inhibited UII stimulated CaN activity by 45% (P < 0.01) and 21% (P < 0.05), respectively, while PD98059 50 mumol/L had no effect on CaN activity (P > 0.05). CsA 10-6 mol/L inhibited UII -stimulated PKC activity by 14% (P < 0.05), while having no effect on MAPK activity (P > 0.05).

Conclusions UII increases cytosolic free calcium concentration and activates PKC, MAPK and CaN. The signal transduction pathway between PKC and CaN has cross-talk.

语种: 英语
WOS记录号: WOS:000189012400008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59174
Appears in Collections:北京大学第三临床医学院_呼吸内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Resp Dis, Beijing 100083, Peoples R China
2.Peking Univ, Teaching Hosp 1, Cardiovasc Res Inst, Beijing 100034, Peoples R China

Recommended Citation:
Chen, YH,Zhao, MW,Yao, WZ,et al. The signal transduction pathway in the proliferation of airway smooth muscle cells induced by urotensin II[J]. CHINESE MEDICAL JOURNAL,2004,117(1):37-41.
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