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Methylation of a Novel CpG Island of Intron 1 Is Associated With Steroidogenic Factor 1 Expression in Endometriotic Stromal Cells
Xue, Qing1; Xu, Yang1; Yang, Huixia1; Zhang, Lei1; Shang, Jing1; Zeng, Cheng1; Yin, Ping2; Bulun, Serdar E.2
关键词SF-1 endometriosis DNA methylation intron CpG island
刊名REPRODUCTIVE SCIENCES
2014-03-01
DOI10.1177/1933719113497283
21期:3页:395-400
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]FACTOR-I ; DNA METHYLATION ; TRANSCRIPTION FACTOR ; RECEPTOR ; GENE ; BINDING ; PROTEIN ; CANCER ; PROMOTERS ; HISTORY
英文摘要

Steroidogenic factor 1 (SF-1), a transcriptional factor essential for estrogen biosynthesis, is undetectable in endometrial stromal cells and aberrantly expressed in endometriotic stromal cells.

Objective: We tried to gain further insight into the mechanism for differential SF-1 expression in endometrial and endometriotic stromal cells.

Design: We had previously identified a novel CpG island in SF-1, which is located in the downstream intron 1 region. Here, we evaluated the methylation status of this CpG island.

Patients: We obtained the eutopic endometrium from disease-free participants (n = 8) and the walls of cystic endometriosis lesions of the ovaries from another group of participants (n = 8). None of the patients had received any preoperative hormonal therapy.

Interventions: Stromal cells were isolated from these 2 types of tissues and subjected to DNA bisulfite treatment and sequence analysis.

Results: The SF-1 messenger RNA (mRNA) levels in endometriotic stromal cells were significantly higher than those in endometrial stromal cells. Bisulfite sequencing showed strikingly increased methylation of a 1-kbp region around the previously identified CpG island in endometriotic cells compared with endometrial cells (P < .001). A strong correlation between SF-1 mRNA levels and percentage methylation of the intron 1 region of the SF-1 gene was observed in endometriotic cells (Spearman correlation coefficient, .96; P < .001).

Conclusions: Methylation of the intron 1 region of the SF-1 gene is associated with its expression in endometriotic cells. This CpG island therefore plays an important role in regulating SF-1 expression.

语种英语
WOS记录号WOS:000331928000012
项目编号30973183 ; 81270674
资助机构Natural Science Foundation of China
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59179
专题北京大学第一临床医学院
北京大学第一临床医学院_妇产科
作者单位1.Beijing Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100034, Peoples R China
2.Northwestern Univ, Feinberg Sch Med, Dept Obstet & Gynecol, Div Reprod Endocrinol & Infertil, Chicago, IL 60611 USA
推荐引用方式
GB/T 7714
Xue, Qing,Xu, Yang,Yang, Huixia,et al. Methylation of a Novel CpG Island of Intron 1 Is Associated With Steroidogenic Factor 1 Expression in Endometriotic Stromal Cells[J]. REPRODUCTIVE SCIENCES,2014,21(3):395-400.
APA Xue, Qing.,Xu, Yang.,Yang, Huixia.,Zhang, Lei.,Shang, Jing.,...&Bulun, Serdar E..(2014).Methylation of a Novel CpG Island of Intron 1 Is Associated With Steroidogenic Factor 1 Expression in Endometriotic Stromal Cells.REPRODUCTIVE SCIENCES,21(3),395-400.
MLA Xue, Qing,et al."Methylation of a Novel CpG Island of Intron 1 Is Associated With Steroidogenic Factor 1 Expression in Endometriotic Stromal Cells".REPRODUCTIVE SCIENCES 21.3(2014):395-400.
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