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学科主题: 临床医学
题名:
Methylation of a Novel CpG Island of Intron 1 Is Associated With Steroidogenic Factor 1 Expression in Endometriotic Stromal Cells
作者: Xue, Qing1; Xu, Yang1; Yang, Huixia1; Zhang, Lei1; Shang, Jing1; Zeng, Cheng1; Yin, Ping2; Bulun, Serdar E.2
关键词: SF-1 ; endometriosis ; DNA methylation ; intron ; CpG island
刊名: REPRODUCTIVE SCIENCES
发表日期: 2014-03-01
DOI: 10.1177/1933719113497283
卷: 21, 期:3, 页:395-400
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: FACTOR-I ; DNA METHYLATION ; TRANSCRIPTION FACTOR ; RECEPTOR ; GENE ; BINDING ; PROTEIN ; CANCER ; PROMOTERS ; HISTORY
英文摘要:

Steroidogenic factor 1 (SF-1), a transcriptional factor essential for estrogen biosynthesis, is undetectable in endometrial stromal cells and aberrantly expressed in endometriotic stromal cells.

Objective: We tried to gain further insight into the mechanism for differential SF-1 expression in endometrial and endometriotic stromal cells.

Design: We had previously identified a novel CpG island in SF-1, which is located in the downstream intron 1 region. Here, we evaluated the methylation status of this CpG island.

Patients: We obtained the eutopic endometrium from disease-free participants (n = 8) and the walls of cystic endometriosis lesions of the ovaries from another group of participants (n = 8). None of the patients had received any preoperative hormonal therapy.

Interventions: Stromal cells were isolated from these 2 types of tissues and subjected to DNA bisulfite treatment and sequence analysis.

Results: The SF-1 messenger RNA (mRNA) levels in endometriotic stromal cells were significantly higher than those in endometrial stromal cells. Bisulfite sequencing showed strikingly increased methylation of a 1-kbp region around the previously identified CpG island in endometriotic cells compared with endometrial cells (P < .001). A strong correlation between SF-1 mRNA levels and percentage methylation of the intron 1 region of the SF-1 gene was observed in endometriotic cells (Spearman correlation coefficient, .96; P < .001).

Conclusions: Methylation of the intron 1 region of the SF-1 gene is associated with its expression in endometriotic cells. This CpG island therefore plays an important role in regulating SF-1 expression.

语种: 英语
所属项目编号: 30973183 ; 81270674
项目资助者: Natural Science Foundation of China
WOS记录号: WOS:000331928000012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59179
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Beijing Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100034, Peoples R China
2.Northwestern Univ, Feinberg Sch Med, Dept Obstet & Gynecol, Div Reprod Endocrinol & Infertil, Chicago, IL 60611 USA

Recommended Citation:
Xue, Qing,Xu, Yang,Yang, Huixia,et al. Methylation of a Novel CpG Island of Intron 1 Is Associated With Steroidogenic Factor 1 Expression in Endometriotic Stromal Cells[J]. REPRODUCTIVE SCIENCES,2014,21(3):395-400.
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