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学科主题: 基础医学
题名:
Both the establishment and maintenance of neuronal polarity require the activity of protein kinase D in the Golgi apparatus
作者: Yin, Dong-Min; Huang, Yan-Hua; Zhu, Yan-Bing; Wang, Yun1
关键词: neuronal polarity ; protein kinase D ; Golgi apparatus ; membrane trafficking ; axon formation ; neuronal morphology
刊名: JOURNAL OF NEUROSCIENCE
发表日期: 2008-08-27
DOI: 10.1523/JNEUROSCI.1291-08.2008
卷: 28, 期:35, 页:8832-8843
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: DENDRITIC SPINE MORPHOGENESIS ; HIPPOCAMPAL-NEURONS ; C-MU ; TRANSPORT CARRIERS ; CORTICAL-NEURONS ; AXON FORMATION ; SAD KINASES ; MEMBRANE ; PHOSPHORYLATION ; POLARIZATION
英文摘要:

Neuronal polarization requires coordinated regulation of membrane trafficking and cytoskeletal dynamics. Several signaling proteins are involved in neuronal polarization via modulation of cytoskeletal dynamics in neurites. However, very little is known about signaling proteins in the neuronal soma, which regulate polarized membrane trafficking and neuronal polarization. Protein kinase D (PKD) constitutes a family of serine/threonine-specific protein kinases and is important in regulating Golgi dynamics and membrane trafficking. Here, we show that two members of the PKD family, PKD1 and PKD2, are essential for the establishment and maintenance of neuronal polarity. Loss of function of PKD with inhibitor, dominant negative, and short interfering RNA disrupts polarized membrane trafficking and induces multiple axon formation. Gain of function of PKD can rescue the disruption of polarized membrane trafficking and neuronal polarity caused by cytochalasin D, which results in F-actin depolymerization. PKD1 and PKD2 are also found to be involved in the maintenance of neuronal polarity, as evidenced by the conversion of preexisting dendrites into axons on PKD inhibition. Unlike other polarity proteins, PKD does not interact with the cytoskeleton in neurites. Instead, PKD regulates neuronal polarity through its activity in the Golgi apparatus. These data reveal a novel mechanism regulating neuronal polarity in the Golgi apparatus.

语种: 英语
所属项目编号: 2007CB512100
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation ; National Basic Research Program
WOS记录号: WOS:000258762800022
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59186
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Key Lab Neurosci, Minist Educ, Beijing 100083, Peoples R China
2.Peking Univ, Dept Neurobiol, Key Lab Neurosci, Minist Educ, Beijing 100083, Peoples R China

Recommended Citation:
Yin, Dong-Min,Huang, Yan-Hua,Zhu, Yan-Bing,et al. Both the establishment and maintenance of neuronal polarity require the activity of protein kinase D in the Golgi apparatus[J]. JOURNAL OF NEUROSCIENCE,2008,28(35):8832-8843.
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