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学科主题基础医学
Both the establishment and maintenance of neuronal polarity require the activity of protein kinase D in the Golgi apparatus
Yin, Dong-Min; Huang, Yan-Hua; Zhu, Yan-Bing; Wang, Yun1
关键词Neuronal Polarity Protein Kinase d Golgi Apparatus Membrane Trafficking Axon Formation Neuronal Morphology
刊名JOURNAL OF NEUROSCIENCE
2008-08-27
DOI10.1523/JNEUROSCI.1291-08.2008
28期:35页:8832-8843
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]DENDRITIC SPINE MORPHOGENESIS ; HIPPOCAMPAL-NEURONS ; C-MU ; TRANSPORT CARRIERS ; CORTICAL-NEURONS ; AXON FORMATION ; SAD KINASES ; MEMBRANE ; PHOSPHORYLATION ; POLARIZATION
英文摘要

Neuronal polarization requires coordinated regulation of membrane trafficking and cytoskeletal dynamics. Several signaling proteins are involved in neuronal polarization via modulation of cytoskeletal dynamics in neurites. However, very little is known about signaling proteins in the neuronal soma, which regulate polarized membrane trafficking and neuronal polarization. Protein kinase D (PKD) constitutes a family of serine/threonine-specific protein kinases and is important in regulating Golgi dynamics and membrane trafficking. Here, we show that two members of the PKD family, PKD1 and PKD2, are essential for the establishment and maintenance of neuronal polarity. Loss of function of PKD with inhibitor, dominant negative, and short interfering RNA disrupts polarized membrane trafficking and induces multiple axon formation. Gain of function of PKD can rescue the disruption of polarized membrane trafficking and neuronal polarity caused by cytochalasin D, which results in F-actin depolymerization. PKD1 and PKD2 are also found to be involved in the maintenance of neuronal polarity, as evidenced by the conversion of preexisting dendrites into axons on PKD inhibition. Unlike other polarity proteins, PKD does not interact with the cytoskeleton in neurites. Instead, PKD regulates neuronal polarity through its activity in the Golgi apparatus. These data reveal a novel mechanism regulating neuronal polarity in the Golgi apparatus.

语种英语
WOS记录号WOS:000258762800022
项目编号2007CB512100
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation ; National Basic Research Program
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被引频次:40[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59186
专题北京大学基础医学院_神经生物学系
作者单位1.Peking Univ, Neurosci Res Inst, Key Lab Neurosci, Minist Educ, Beijing 100083, Peoples R China
2.Peking Univ, Dept Neurobiol, Key Lab Neurosci, Minist Educ, Beijing 100083, Peoples R China
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GB/T 7714
Yin, Dong-Min,Huang, Yan-Hua,Zhu, Yan-Bing,et al. Both the establishment and maintenance of neuronal polarity require the activity of protein kinase D in the Golgi apparatus[J]. JOURNAL OF NEUROSCIENCE,2008,28(35):8832-8843.
APA Yin, Dong-Min,Huang, Yan-Hua,Zhu, Yan-Bing,&Wang, Yun.(2008).Both the establishment and maintenance of neuronal polarity require the activity of protein kinase D in the Golgi apparatus.JOURNAL OF NEUROSCIENCE,28(35),8832-8843.
MLA Yin, Dong-Min,et al."Both the establishment and maintenance of neuronal polarity require the activity of protein kinase D in the Golgi apparatus".JOURNAL OF NEUROSCIENCE 28.35(2008):8832-8843.
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