IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Target-directed screening of the bioactive compounds specifically binding to (2)-adrenoceptor in Semen brassicae by high-performance affinity chromatography
An, Yuxin1; Li, Xia2; Sun, Huanmei1; Bian, Wenhai3; Li, Zijian4,5; Zhang, Youyi4,5; Zhao, Xinfeng1; Zheng, Xiaohui1
关键词high-performance affinity chromatography molecular docking immobilized (2)-AR drug screening Semen brassicae
刊名JOURNAL OF MOLECULAR RECOGNITION
2015-10-01
DOI10.1002/jmr.2478
28期:10页:628-634
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
资助者National Natural Sciences Foundation of China ; Program for Changjiang Scholars and Innovative Research Team in University ; Innovative Research Team of Research and Technology of Shaanxi Province ; Ministry of Science and Technology of the China ; Natural Science Basic Research Plan in Shaanxi Province of China ; National Natural Sciences Foundation of China ; Program for Changjiang Scholars and Innovative Research Team in University ; Innovative Research Team of Research and Technology of Shaanxi Province ; Ministry of Science and Technology of the China ; Natural Science Basic Research Plan in Shaanxi Province of China
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]HUMAN SERUM-ALBUMIN ; NATURAL-PRODUCTS ; PROTEIN BINDING ; BETA(2)-ADRENOCEPTOR ; MECHANISMS ; RHIZOMA ; ASTHMA ; ONLINE
英文摘要

The bioactive ingredients in Semen sinapis were rapidly screened by immobilized (2)-adrenoceptor ((2)-AR) and target-directed molecular docking. The methods involved the attachment of (2)-AR using any amino group in the receptor, the simultaneous separation and identification of the retention compounds by high-performance affinity chromatography; the binding mechanism of the interesting compound to the receptor was investigated by zonal elution and molecular docking. Sinapine in Semen sinapis was proved to be the bioactive compound that specifically binds to the immobilized receptor. The association constant of sinapine to (2)-AR was determined to be 1.36x10(5)M(-1) with a value of 1.27x10(-6)M for the number of binding sites. Ionic bond was believed to be the driving force during the interaction between sinapine and (2)-AR. It is possible to become a powerful alternative for rapid screening of bioactive compounds from a complex matrix such as traditional Chinese medicine and further investigation on the drug-receptor interaction. Copyright (c) 2015 John Wiley & Sons, Ltd.

语种英语
所属项目编号J1210063 ; 21475103 ; IRT1174 ; 2013KCT-24 ; 2013YQ17052509 ; 2013JM4024
资助者National Natural Sciences Foundation of China ; Program for Changjiang Scholars and Innovative Research Team in University ; Innovative Research Team of Research and Technology of Shaanxi Province ; Ministry of Science and Technology of the China ; Natural Science Basic Research Plan in Shaanxi Province of China ; National Natural Sciences Foundation of China ; Program for Changjiang Scholars and Innovative Research Team in University ; Innovative Research Team of Research and Technology of Shaanxi Province ; Ministry of Science and Technology of the China ; Natural Science Basic Research Plan in Shaanxi Province of China
WOS记录号WOS:000361224400004
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59207
Collection北京大学第三临床医学院_心血管内科
作者单位1.Shaanxi Inst Food & Drug Control, Xian 710065, Peoples R China
2.Northwest Univ, Coll Life Sci, Xian 710069, Peoples R China
3.Northwest Univ, Affiliated High Sch, Xian 710075, Peoples R China
4.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
5.Peking Univ, Key Lab Mol Cardiovasc Sci, Minist Educ, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
An, Yuxin,Li, Xia,Sun, Huanmei,et al. Target-directed screening of the bioactive compounds specifically binding to (2)-adrenoceptor in Semen brassicae by high-performance affinity chromatography[J]. JOURNAL OF MOLECULAR RECOGNITION,2015,28(10):628-634.
APA An, Yuxin.,Li, Xia.,Sun, Huanmei.,Bian, Wenhai.,Li, Zijian.,...&Zheng, Xiaohui.(2015).Target-directed screening of the bioactive compounds specifically binding to (2)-adrenoceptor in Semen brassicae by high-performance affinity chromatography.JOURNAL OF MOLECULAR RECOGNITION,28(10),628-634.
MLA An, Yuxin,et al."Target-directed screening of the bioactive compounds specifically binding to (2)-adrenoceptor in Semen brassicae by high-performance affinity chromatography".JOURNAL OF MOLECULAR RECOGNITION 28.10(2015):628-634.
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