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学科主题: 临床医学
题名:
Autologous transplantation of RPE with partial-thickness choroid after mechanical debridement of Bruch membrane in the rabbit
作者: Hu, Yuntao1; Zhang, Taoran1; Wu, Jianguo1; Li, Ying1; Lu, Xinrong1; Qian, Fang1; Yin, Zhengqin2; Ma, Zhizhong1
刊名: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
发表日期: 2008-07-01
DOI: 10.1167/iovs.07-1299
卷: 49, 期:7, 页:3185-3192
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: RETINAL-PIGMENT EPITHELIUM ; MACULAR TRANSLOCATION SURGERY ; RANDOMIZED CLINICAL-TRIAL ; 360-DEGREE PERIPHERAL RETINECTOMY ; SUBFOVEAL NEOVASCULAR MEMBRANES ; FIBROBLAST GROWTH-FACTOR ; OUTER SEGMENT MEMBRANES ; ANTERIOR LENS CAPSULE ; PHOTODYNAMIC THERAPY ; SURGICAL REMOVAL
英文摘要:

PURPOSE. An improved translocation technique for autologous retinal pigment epithelium ( RPE) transplantation is presented. The graft consists of a sheet of a partial-thickness choroid with RPE attached.

METHODS. Twenty-seven pigmented rabbits were used in this study. After mechanical debridement of Bruch membrane,partialthickness RPE- choroid sheets were transplanted onto the subretinal space in 25 rabbits. The animals were examined by fundus photographs and fluorescein angiographs and were killed postoperatively at 1, 2, 4, 12, and 24 weeks. Eyecups containing the grafts were examined by light microscopy and immunohistochemistry. In addition, two partial-thickness RPE-choroid sheets were analyzed by transmission electron microscopy ( TEM).

RESULTS. TEM revealed that the partial-thickness RPE- choroid graft consisted of retinal pigment epithelial cells, Bruch membrane, choriocapillaris, and ruptured middle vessels. The thickness of the graft was approximately 50 to 60 mu m. Fluorescein angiography revealed neither fluorescein leakage nor staining in the graft at early or late phase. Light microscopy revealed that in 17 experiments in which the graft survived, the neural retina remained intact; however, in eight experiments with unsuccessful grafts, the neural retina degenerated. The surviving graft showed revascularization and monolayered retinal pigment epithelial cells. Furthermore, in sections in which the neural retina over the graft remained intact, all retinal pigment epithelial cells in the graft and rhodopsin in photoreceptor outer segments were positively labeled with anticellular retinaldehy-debinding protein antibodies and anti-opsin antibodies, respectively.

CONCLUSIONS. A partial-thickness RPE- choroid graft showed improved integration with the host choroid and photoreceptors. This technique has the potential to be a treatment for age-related macular degeneration.

语种: 英语
WOS记录号: WOS:000257124000053
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59211
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Ctr Eye, Hosp 3, Beijing 100083, Peoples R China
2.Third Mil Med Univ, SW Eye Hosp, Chongqing, Peoples R China

Recommended Citation:
Hu, Yuntao,Zhang, Taoran,Wu, Jianguo,et al. Autologous transplantation of RPE with partial-thickness choroid after mechanical debridement of Bruch membrane in the rabbit[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2008,49(7):3185-3192.
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