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Autologous transplantation of RPE with partial-thickness choroid after mechanical debridement of Bruch membrane in the rabbit
Hu, Yuntao1; Zhang, Taoran1; Wu, Jianguo1; Li, Ying1; Lu, Xinrong1; Qian, Fang1; Yin, Zhengqin2; Ma, Zhizhong1
刊名INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
2008-07-01
DOI10.1167/iovs.07-1299
49期:7页:3185-3192
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]RETINAL-PIGMENT EPITHELIUM ; MACULAR TRANSLOCATION SURGERY ; RANDOMIZED CLINICAL-TRIAL ; 360-DEGREE PERIPHERAL RETINECTOMY ; SUBFOVEAL NEOVASCULAR MEMBRANES ; FIBROBLAST GROWTH-FACTOR ; OUTER SEGMENT MEMBRANES ; ANTERIOR LENS CAPSULE ; PHOTODYNAMIC THERAPY ; SURGICAL REMOVAL
英文摘要

PURPOSE. An improved translocation technique for autologous retinal pigment epithelium ( RPE) transplantation is presented. The graft consists of a sheet of a partial-thickness choroid with RPE attached.

METHODS. Twenty-seven pigmented rabbits were used in this study. After mechanical debridement of Bruch membrane,partialthickness RPE- choroid sheets were transplanted onto the subretinal space in 25 rabbits. The animals were examined by fundus photographs and fluorescein angiographs and were killed postoperatively at 1, 2, 4, 12, and 24 weeks. Eyecups containing the grafts were examined by light microscopy and immunohistochemistry. In addition, two partial-thickness RPE-choroid sheets were analyzed by transmission electron microscopy ( TEM).

RESULTS. TEM revealed that the partial-thickness RPE- choroid graft consisted of retinal pigment epithelial cells, Bruch membrane, choriocapillaris, and ruptured middle vessels. The thickness of the graft was approximately 50 to 60 mu m. Fluorescein angiography revealed neither fluorescein leakage nor staining in the graft at early or late phase. Light microscopy revealed that in 17 experiments in which the graft survived, the neural retina remained intact; however, in eight experiments with unsuccessful grafts, the neural retina degenerated. The surviving graft showed revascularization and monolayered retinal pigment epithelial cells. Furthermore, in sections in which the neural retina over the graft remained intact, all retinal pigment epithelial cells in the graft and rhodopsin in photoreceptor outer segments were positively labeled with anticellular retinaldehy-debinding protein antibodies and anti-opsin antibodies, respectively.

CONCLUSIONS. A partial-thickness RPE- choroid graft showed improved integration with the host choroid and photoreceptors. This technique has the potential to be a treatment for age-related macular degeneration.

语种英语
WOS记录号WOS:000257124000053
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59211
专题北京大学第三临床医学院_眼科
北京大学基础医学院
作者单位1.Peking Univ, Ctr Eye, Hosp 3, Beijing 100083, Peoples R China
2.Third Mil Med Univ, SW Eye Hosp, Chongqing, Peoples R China
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GB/T 7714
Hu, Yuntao,Zhang, Taoran,Wu, Jianguo,et al. Autologous transplantation of RPE with partial-thickness choroid after mechanical debridement of Bruch membrane in the rabbit[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2008,49(7):3185-3192.
APA Hu, Yuntao.,Zhang, Taoran.,Wu, Jianguo.,Li, Ying.,Lu, Xinrong.,...&Ma, Zhizhong.(2008).Autologous transplantation of RPE with partial-thickness choroid after mechanical debridement of Bruch membrane in the rabbit.INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,49(7),3185-3192.
MLA Hu, Yuntao,et al."Autologous transplantation of RPE with partial-thickness choroid after mechanical debridement of Bruch membrane in the rabbit".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 49.7(2008):3185-3192.
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