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学科主题: 临床医学
题名:
Effects of penicillin-induced developmental epilepticus on hippocampal regenerative sprouting, related gene expression and cognitive deficits in rats
作者: Ni, Hong1,2; Jiang, Yu-wu3; Tao, Lu-yuang1,2; Cen, Jian-nong1,2; Wu, Xi-ru3
关键词: Zinc transporter ; CaMKII alpha ; NR2C ; GluR2 ; Seizure ; Mossy fiber sprouting
刊名: TOXICOLOGY LETTERS
发表日期: 2009-07-24
DOI: 10.1016/j.toxlet.2009.04.002
卷: 188, 期:2, 页:161-166
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: RECURRENT NEONATAL SEIZURES ; WISTAR RAT ; CAMK-II ; MEMORY ; ZINC ; MICE ; EPILEPSY ; BRAIN ; TRAFFICKING
英文摘要:

For the purpose of investigating the long-term effects of seizures in developmental rats on spatial learning ability and hippocampal mossy fiber sprouting related gene expressions in adult rat brain, a seizure was induced by penicillin quaque die alterna in Sprague-Dawley rats from postnatal day 29 (P29). Rats were assigned into the recurrent seizure group (RS, seizures were induced in 11 consecutive days) and the control group. During P51-P56, P81-1384 and P92-P95, the rats were tested for spatial learning ability with the Morris water maze task. On P95, the authors examined mossy fiber sprouting and gene expression of zinc transporters 1 and 3 (ZnT-1, ZnT-3), calcium/calmodulin-dependent protein kinase II alpha (CaMKII alpha), NMDA receptor 2C (NR2C) and glutamate receptor 2 (GluR2) in hippocampus by Timm staining and real-time RT-PCR analysis. The escape latencies from the water maze of the rats in the RS group were significantly longer than those of the control rats at d5 of the first test, at d1 of the second test, and at d2 of the third test. In the spatial probe test, the ratio between the swim time in the third quadrant and the total swim time in control group was significantly higher than RS group (p < 0.05) in the entire three probe tests. The Timm scores in CA3 and dentate gyrus in the RS animals were significantly higher than that in the control. Compared with the control rats, the expressions of ZnT-1, CaMK-II alpha and GluR2 transcripts in the hippocampus of the RS group was significantly decreased while unchanged in transcriptional levels of ZnT-3 and NR2C. There were positive linear correlations among ZnT-3, CaMKII alpha, and NR2C in control group and among CaMKII alpha, ZnT-1 and GluR2 in RS group. The results suggest that recurrent seizures induced in developmental rats could cause long-term disturbance on the hippocampal mossy fiber sprouting related gene expressions, which might play an important role in long-term cognitive deficit and hippocampal aberrant mossy fiber sprouting. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 30470555 ; 30870808 ; 30872666 ; 2003034094 ; BK2007509 ; 07KJB320103
项目资助者: National Natural Science Foundation of China ; 34th Postdoctoral Science Foundation of China ; Natural Science Foundation of Jiangsu Province of China
WOS记录号: WOS:000266734400014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59222
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Soochow Univ, Neurol Lab, Childrens Hosp, Suzhou 215003, Peoples R China
2.Soochow Univ, Lab Aging & Nervous Dis, Suzhou 215003, Peoples R China
3.Peking Univ, Dept Pediat, Affiliated Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Ni, Hong,Jiang, Yu-wu,Tao, Lu-yuang,et al. Effects of penicillin-induced developmental epilepticus on hippocampal regenerative sprouting, related gene expression and cognitive deficits in rats[J]. TOXICOLOGY LETTERS,2009,188(2):161-166.
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