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学科主题: 药学
题名:
Development of intron length polymorphism markers in genes encoding diketide-CoA synthase and curcumin synthase for discriminating Curcuma species
作者: Kita, Tomoko1; Komatsu, Katsuko2; Zhu, Shu2; Iida, Osamu3; Sugimura, Koji4; Kawahara, Nobuo3; Taguchi, Hiromu1; Masamura, Noriya1; Cai, Shao-Qing5
关键词: Curcuma ; Intron length polymorphism ; Curcumin synthase ; trnK ; Molecular identification
刊名: FOOD CHEMISTRY
发表日期: 2016-03-01
DOI: 10.1016/j.foodchem.2015.08.034
卷: 194, 页:1329-1336
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
研究领域[WOS]: Chemistry ; Food Science & Technology ; Nutrition & Dietetics
关键词[WOS]: RIBOSOMAL-RNA GENE ; TRNK GENE ; LONGA ; CHINESE ; SATIVA ; DRUGS ; L.
英文摘要:

Various Curcuma rhizomes have been used as medicines or spices in Asia since ancient times. It is very difficult to distinguish them morphologically, especially when they are boiled and dried, which causes misidentification leading to a loss of efficacy. We developed a method for discriminating Curcuma species by intron length polymorphism markers in genes encoding diketide-CoA synthase and curcumin synthase. This method could apply to identification of not only fresh plants but also samples of crude drugs or edible spices. By applying this method to Curcuma specimens and samples, and constructing a dendrogram based on these markers, seven Curcuma species were clearly distinguishable. Moreover, Curcuma longa specimens were geographically distinguishable. On the other hand, Curcuma kwangsiensis (gl type) specimens also showed intraspecies polymorphism, which may have occurred as a result of hybridization with other Curcuma species. The molecular method we developed is a potential tool for global classification of the genus Curcuma. (C) 2015 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 24406005
项目资助者: JSPS KAKENHI
WOS记录号: WOS:000364248900171
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59233
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.House Foods Grp Inc, Cent Res & Dev Inst, Chiba 2840033, Japan
2.Toyama Univ, Inst Nat Med, Dept Med Resources, Div Pharmacognosy, Toyama 9300194, Japan
3.Natl Inst Biomed Innovat, Tsukuba Div, Res Ctr Med Plant Resources, Tsukuba, Ibaraki 3050843, Japan
4.Natl Inst Biomed Innovat, Tanegashima Div, Res Ctr Med Plant Resources, Nakatane, Kagoshima 8913604, Japan
5.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Kita, Tomoko,Komatsu, Katsuko,Zhu, Shu,et al. Development of intron length polymorphism markers in genes encoding diketide-CoA synthase and curcumin synthase for discriminating Curcuma species[J]. FOOD CHEMISTRY,2016,194:1329-1336.
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