IR@PKUHSC  > 北京大学第一临床医学院  > 感染疾病科
学科主题临床医学
Molecular Analysis of Anaplasma phagocytophilum Isolated from Patients with Febrile Diseases of Unknown Etiology in China
Zhang, Lijuan1; Wang, Guiqiang2; Liu, Qinghui3; Chen, Chuangfu4; Li, Jun2; Long, Bo5; Yu, Hong5; Zhang, Zhilun6; He, Jing7; Qu, Zhangyi8; Yu, Jiguang5; Liu, Yuanni5; Dong, Tuo1,8; Yao, Na1,4; Wang, Yong1,4; Cheng, Xueqin1; Xu, Jianguo1
刊名PLOS ONE
2013-02-22
DOI10.1371/journal.pone.0057155
8期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]HUMAN GRANULOCYTIC ANAPLASMOSIS ; EHRLICHIA-CHAFFEENSIS ; PHYLOGENETIC ANALYSIS ; INFECTION ; TICKS ; SEQUENCE ; OUTCOMES ; JAPAN
英文摘要

Although anaplasmosis cases have been nationally identified in China, no human isolates of A. phagocytophilum have been obtained, which limits the analysis of any molecular and genetic contributions to patients′ severe clinical manifestations and the study of the bacteria′s pathogeneses in China. Given this situation, a joint project was conducted in 2009-2010. A total of 421 febrile cases of unknown etiology were collected and the patients′ blood samples were collected for laboratory diagnoses including serologic diagnosis based on the four-fold rise in the anti- A. phagocytophilum IgG titer by indirect micro-immunofluorescence assay (IFA), positive PCR assay and confirmation of A. phagocytophilum DNA and positive culture of A. phagocytophilum and confirmed by amplification and sequencing of the 16S rRNA and ank A genes of the A. phagocytophilum isolates. A total of 570 ticks were collected from the patients′ domestic animals (456) and from wild fields (114) for culturing and amplifying and sequencing the 16S rRNA gene of A. phagocytophilum. Phylogenetic analyses were performed on the 16S rRNA and ank A gene sequences of the isolates and the ticks tested in the study. A total of 46 (10.9%) confirmed and 16 (3.8%) probable cases were diagnosed and severe clinical features and higher mortality rates were observed in these Chinese patients. Five isolates were obtained and the 16S rRNA genes of the 5 isolates were conserved but variety for ank A genes. Two human isolates and 1 tick isolate from Shandong Peninsula, where all patients exhibited severe clinical manifestations, were grouped as one clan based on the phylogenetic analyses, while 2 other human isolates were clustered in a second clan. 43.5% of H. longicornis were infected with A. phagocytophilum. The present study is the first to obtain clinical isolates of A. phagocytophilum in China. The diversity of the ank A genes of Chinese isolates will help us to further discern the relationship between the variations in the ank A genes and the severity of the disease′s clinical manifestations in China.

语种英语
WOS记录号WOS:000316658800081
项目编号2010CB530206 ; 30771854 ; 1U2GGH000018-01 ; 2011ZX10004-001 ; 20122X10004215
资助机构National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; China-U.S. Collaborative Program on Emerging and Re-emerging Infectious Diseases ; China Mega-Project for Infectious Disease ; National Key Science and Technology Projects of China
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59251
专题北京大学第一临床医学院_感染疾病科
作者单位1.Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Dept Rickettsiol, Beijing, Peoples R China
2.Peking Univ First Hosp, Dept Infect Dis, Beijing, Peoples R China
3.Shandong Laizhou Peoples Hosp, Dept Intens Care, Laizhou, Shandong, Peoples R China
4.Shihezi Univ, Anim Sci & Technol Coll, Shihezi, Xinjiang Provin, Peoples R China
5.302 Hosp PLA, Dept Infect Dis, Beijing, Peoples R China
6.Yantai Infect Dis Hosp, Dept Infect Dis, Yantai, Shandong, Peoples R China
7.Tianjin Ctr Dis Control & Prevent, Dept Epidemiol, Tianjin, Peoples R China
8.Harbin Med Univ, Sch Publ Hlth, Harbin, Heilongjiang Pr, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Lijuan,Wang, Guiqiang,Liu, Qinghui,et al. Molecular Analysis of Anaplasma phagocytophilum Isolated from Patients with Febrile Diseases of Unknown Etiology in China[J]. PLOS ONE,2013,8(2).
APA Zhang, Lijuan.,Wang, Guiqiang.,Liu, Qinghui.,Chen, Chuangfu.,Li, Jun.,...&Xu, Jianguo.(2013).Molecular Analysis of Anaplasma phagocytophilum Isolated from Patients with Febrile Diseases of Unknown Etiology in China.PLOS ONE,8(2).
MLA Zhang, Lijuan,et al."Molecular Analysis of Anaplasma phagocytophilum Isolated from Patients with Febrile Diseases of Unknown Etiology in China".PLOS ONE 8.2(2013).
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