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学科主题临床医学
ENDOPLASMIC RETICULUM STRESS INVOLVED IN HEART AND LIVER INJURY IN IRON-LOADED RATS
Lou, Li-Xia1,3; Geng, Bin1,2; Chen, Yu2; Yu, Fang2; Zhao, Jing2; Tang, Chao-Shu1,2
关键词endoplasmic reticulum stress iron load oxidative stress rat
刊名CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
2009-07-01
DOI10.1111/j.1440-1681.2008.05114.x
36期:7页:612-618
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy ; Physiology
资助者Major State Basic Research Development Program of the People&prime ; s Republic of China ; Major State Basic Research Development Program of the People&prime ; s Republic of China
研究领域[WOS]Pharmacology & Pharmacy ; Physiology
关键词[WOS]UNFOLDED PROTEIN RESPONSE ; MEDIATED FENTON REACTIONS ; CELL-DEATH PROGRAM ; OXIDATIVE DAMAGE ; LIPID-PEROXIDATION ; DNA CONSTITUENTS ; TISSUE-INJURY ; ER STRESS ; OVERLOAD ; DISEASE
英文摘要

P>Iron overload contributes to the pathogenesis of various diseases and directly induces tissue injury. In the present study, we investigated the relationship between heart and liver injury induced by iron overload and cellular endoplasmic reticulum (ER) stress to explore the molecular mechanism of iron overload-induced cellular injury.

Iron overload in rats was generated by intraperitoneal injection of iron-dextran chronically (30 mg/kg per day for 9 weeks) or acutely (300 mg/kg once). Tissue injury was assessed by determining serum lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity, as well as malondialdehyde (MDA) content in the heart and liver. The ER stress response was analysed by expression of glucose-response protein 78 (GRP78) and activation of caspase 12.

In chronic iron-loaded rats, iron levels in the heart and liver were higher, by approximately 2-and 7.8-fold, respectively (P < 0.01), compared with control. Serum LDH, ALT and AST activity, as well as MDA content, GRP78 expression and caspase 12 activity in the heart and liver, were upregulated in chronically iron-loaded rats. In acute iron-loaded rats, iron content in the heart and liver was 51% and 63% higher than in controls (both P < 0.01). Serum LDH, ALT and AST activity, MDA content in the heart and liver and levels of ER stress markers were all increased in acute iron-loaded rats. N-Acetylcysteine (150 mg/kg, s.c.) lowered the levels of these parameters in acute iron-loaded rats.

The results of the present study indicate that ER stress may play an important role in iron-induced tissue injury and that reactive oxygen species may mediate the ER stress response in the pathogenesis of iron-overload cellular injury.

语种英语
所属项目编号2006CB503807
资助者Major State Basic Research Development Program of the People&prime ; s Republic of China ; Major State Basic Research Development Program of the People&prime ; s Republic of China
WOS记录号WOS:000267658100002
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59252
专题北京大学第一临床医学院_心血管内科
作者单位1.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100034, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100034, Peoples R China
3.Beijing Univ Ofhinese Med, Dongzhimen Hosp, Educ Minist, Key Lab Chinese Internal Med, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Lou, Li-Xia,Geng, Bin,Chen, Yu,et al. ENDOPLASMIC RETICULUM STRESS INVOLVED IN HEART AND LIVER INJURY IN IRON-LOADED RATS[J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,2009,36(7):612-618.
APA Lou, Li-Xia,Geng, Bin,Chen, Yu,Yu, Fang,Zhao, Jing,&Tang, Chao-Shu.(2009).ENDOPLASMIC RETICULUM STRESS INVOLVED IN HEART AND LIVER INJURY IN IRON-LOADED RATS.CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,36(7),612-618.
MLA Lou, Li-Xia,et al."ENDOPLASMIC RETICULUM STRESS INVOLVED IN HEART AND LIVER INJURY IN IRON-LOADED RATS".CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY 36.7(2009):612-618.
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