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学科主题: 临床医学
题名:
ENDOPLASMIC RETICULUM STRESS INVOLVED IN HEART AND LIVER INJURY IN IRON-LOADED RATS
作者: Lou, Li-Xia1,3; Geng, Bin1,2; Chen, Yu2; Yu, Fang2; Zhao, Jing2; Tang, Chao-Shu1,2
关键词: endoplasmic reticulum stress ; iron load ; oxidative stress ; rat
刊名: CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
发表日期: 2009-07-01
DOI: 10.1111/j.1440-1681.2008.05114.x
卷: 36, 期:7, 页:612-618
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Physiology
研究领域[WOS]: Pharmacology & Pharmacy ; Physiology
关键词[WOS]: UNFOLDED PROTEIN RESPONSE ; MEDIATED FENTON REACTIONS ; CELL-DEATH PROGRAM ; OXIDATIVE DAMAGE ; LIPID-PEROXIDATION ; DNA CONSTITUENTS ; TISSUE-INJURY ; ER STRESS ; OVERLOAD ; DISEASE
英文摘要:

P>Iron overload contributes to the pathogenesis of various diseases and directly induces tissue injury. In the present study, we investigated the relationship between heart and liver injury induced by iron overload and cellular endoplasmic reticulum (ER) stress to explore the molecular mechanism of iron overload-induced cellular injury.

Iron overload in rats was generated by intraperitoneal injection of iron-dextran chronically (30 mg/kg per day for 9 weeks) or acutely (300 mg/kg once). Tissue injury was assessed by determining serum lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity, as well as malondialdehyde (MDA) content in the heart and liver. The ER stress response was analysed by expression of glucose-response protein 78 (GRP78) and activation of caspase 12.

In chronic iron-loaded rats, iron levels in the heart and liver were higher, by approximately 2-and 7.8-fold, respectively (P < 0.01), compared with control. Serum LDH, ALT and AST activity, as well as MDA content, GRP78 expression and caspase 12 activity in the heart and liver, were upregulated in chronically iron-loaded rats. In acute iron-loaded rats, iron content in the heart and liver was 51% and 63% higher than in controls (both P < 0.01). Serum LDH, ALT and AST activity, MDA content in the heart and liver and levels of ER stress markers were all increased in acute iron-loaded rats. N-Acetylcysteine (150 mg/kg, s.c.) lowered the levels of these parameters in acute iron-loaded rats.

The results of the present study indicate that ER stress may play an important role in iron-induced tissue injury and that reactive oxygen species may mediate the ER stress response in the pathogenesis of iron-overload cellular injury.

语种: 英语
所属项目编号: 2006CB503807
项目资助者: Major State Basic Research Development Program of the People&prime ; s Republic of China
WOS记录号: WOS:000267658100002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59252
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100034, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100034, Peoples R China
3.Beijing Univ Ofhinese Med, Dongzhimen Hosp, Educ Minist, Key Lab Chinese Internal Med, Beijing, Peoples R China

Recommended Citation:
Lou, Li-Xia,Geng, Bin,Chen, Yu,et al. ENDOPLASMIC RETICULUM STRESS INVOLVED IN HEART AND LIVER INJURY IN IRON-LOADED RATS[J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,2009,36(7):612-618.
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