北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学深圳医院  > 泌尿外科  > 期刊论文
学科主题: 临床医学
题名:
The EDA-containing cellular fibronectin induces epithelial-mesenchymal transition in lung cancer cells through integrin alpha 9 beta 1-mediated activation of PI3-K/AKT and Erk1/2 (Retracted article. See vol. 36, pg. 1452, 2015)
作者: Sun, Xiaojuan1,2; Fa, Pingping3; Cui, Zhiwen3,4; Xia, Ye3,4; Sun, Liang1; Li, Zesong1; Tang, Aifa1; Gui, Yaoting3; Cai, Zhiming1
刊名: CARCINOGENESIS
发表日期: 2014
DOI: 10.1093/carcin/bgt276
卷: 35, 期:1, 页:184-191
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: EXTRACELLULAR-MATRIX ; CARCINOMA-CELLS ; EIIIA SEGMENT ; EXPRESSION ; PROLIFERATION ; FIBROBLASTS ; TISSUE ; PROGRESSION ; METASTASIS ; INVASION
英文摘要:

Cellular fibronectin (cFN) is one of the main components of tissue extracellular matrices and is involved in multiple physiologic and pathologic processes such as embryogenesis, wound healing, inflammation and tumor progression. The function of fibronectin in regulating normal cell adhesion and migration is well documented, but its function in cancer progression is only partially unraveled. We have reported previously that fibronectin stimulates the proliferation and survival of non-small lung carcinoma cells through upregulation of pro-oncogenic signals related to cyclooxygenase-2/phosphatidylinositol-3-kinase/protein kinase B (COX-2/PI3-K/AKT)/mammalian target of rapamycin triggered by activation of the integrin alpha 5 beta 1. Here, we extend these studies by showing that fibronectin promotes epithelial-mesenchymal transition (EMT) in lung cancer cells. We found that cFN, but not plasma fibronectin or type 1 collagen, induces lung carcinoma cell scattering in vitro, promotes cell migration and invasion of Matrigel and stimulates the expression of the mesenchymal marker alpha-smooth muscle actin while decreasing the expression of the epithelial marker E-cadherin through PI3-K and Erk pathways. Interestingly, the extra domain A (EDA) within cFN was found to be crucial for this process, as confirmed by testing cells overexpressing EDA or cells exposed to EDA-containing matrices. We found that the integrin alpha 9, but not alpha 5, mediated cFN-induced EMT as silencing integrin alpha 9 neutralized cFN-induced EMT. Overall, our findings show that the EDA domain within cFN induces EMT in lung carcinoma cells through integrin alpha 9-mediated activation of PI3-K and Erk.

语种: 英语
所属项目编号: 81101584 ; 81372507 ; 2014CB745200 ; KQC201106020032A ; JCYJ20120614155816670
项目资助者: National Natural Science Foundation of China ; National Natural Science Foundation of China General Program ; Major State Basic Research Development Program of China (973 Program) ; Shenzhen Peacock Plan for Higher Level Experts Aboard ; Major Basic Research Development Program of Shenzhen
WOS记录号: WOS:000329130600022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59253
Appears in Collections:北京大学深圳医院_泌尿外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Tumor Clin Immune Gene Therapy Engn Lab, Dept Biobank,Shenzhen Peoples Hosp 2, Shenzhen 518035, Peoples R China
2.Guangdong Med Coll, Affiliated Hosp, Dept Cent Lab, Zhanjiang 524023, Peoples R China
3.Peking Univ, Shenzhen Hosp, Inst Urol, Shenzhen PKU HKUST Med Ctr,Guangdong & Shenzhen K, Shenzhen 518036, Peoples R China
4.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen 518036, Peoples R China

Recommended Citation:
Sun, Xiaojuan,Fa, Pingping,Cui, Zhiwen,et al. The EDA-containing cellular fibronectin induces epithelial-mesenchymal transition in lung cancer cells through integrin alpha 9 beta 1-mediated activation of PI3-K/AKT and Erk1/2 (Retracted article. See vol. 36, pg. 1452, 2015)[J]. CARCINOGENESIS,2014,35(1):184-191.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Sun, Xiaojuan]'s Articles
[Fa, Pingping]'s Articles
[Cui, Zhiwen]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Sun, Xiaojuan]‘s Articles
[Fa, Pingping]‘s Articles
[Cui, Zhiwen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace