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学科主题临床医学
The EDA-containing cellular fibronectin induces epithelial-mesenchymal transition in lung cancer cells through integrin alpha 9 beta 1-mediated activation of PI3-K/AKT and Erk1/2 (Retracted article. See vol. 36, pg. 1452, 2015)
Sun, Xiaojuan1,2; Fa, Pingping3; Cui, Zhiwen3,4; Xia, Ye3,4; Sun, Liang1; Li, Zesong1; Tang, Aifa1; Gui, Yaoting3; Cai, Zhiming1
刊名CARCINOGENESIS
2014
DOI10.1093/carcin/bgt276
35期:1页:184-191
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China General Program ; Major State Basic Research Development Program of China (973 Program) ; Shenzhen Peacock Plan for Higher Level Experts Aboard ; Major Basic Research Development Program of Shenzhen ; National Natural Science Foundation of China ; National Natural Science Foundation of China General Program ; Major State Basic Research Development Program of China (973 Program) ; Shenzhen Peacock Plan for Higher Level Experts Aboard ; Major Basic Research Development Program of Shenzhen
研究领域[WOS]Oncology
关键词[WOS]EXTRACELLULAR-MATRIX ; CARCINOMA-CELLS ; EIIIA SEGMENT ; EXPRESSION ; PROLIFERATION ; FIBROBLASTS ; TISSUE ; PROGRESSION ; METASTASIS ; INVASION
英文摘要

Cellular fibronectin (cFN) is one of the main components of tissue extracellular matrices and is involved in multiple physiologic and pathologic processes such as embryogenesis, wound healing, inflammation and tumor progression. The function of fibronectin in regulating normal cell adhesion and migration is well documented, but its function in cancer progression is only partially unraveled. We have reported previously that fibronectin stimulates the proliferation and survival of non-small lung carcinoma cells through upregulation of pro-oncogenic signals related to cyclooxygenase-2/phosphatidylinositol-3-kinase/protein kinase B (COX-2/PI3-K/AKT)/mammalian target of rapamycin triggered by activation of the integrin alpha 5 beta 1. Here, we extend these studies by showing that fibronectin promotes epithelial-mesenchymal transition (EMT) in lung cancer cells. We found that cFN, but not plasma fibronectin or type 1 collagen, induces lung carcinoma cell scattering in vitro, promotes cell migration and invasion of Matrigel and stimulates the expression of the mesenchymal marker alpha-smooth muscle actin while decreasing the expression of the epithelial marker E-cadherin through PI3-K and Erk pathways. Interestingly, the extra domain A (EDA) within cFN was found to be crucial for this process, as confirmed by testing cells overexpressing EDA or cells exposed to EDA-containing matrices. We found that the integrin alpha 9, but not alpha 5, mediated cFN-induced EMT as silencing integrin alpha 9 neutralized cFN-induced EMT. Overall, our findings show that the EDA domain within cFN induces EMT in lung carcinoma cells through integrin alpha 9-mediated activation of PI3-K and Erk.

语种英语
所属项目编号81101584 ; 81372507 ; 2014CB745200 ; KQC201106020032A ; JCYJ20120614155816670
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China General Program ; Major State Basic Research Development Program of China (973 Program) ; Shenzhen Peacock Plan for Higher Level Experts Aboard ; Major Basic Research Development Program of Shenzhen ; National Natural Science Foundation of China ; National Natural Science Foundation of China General Program ; Major State Basic Research Development Program of China (973 Program) ; Shenzhen Peacock Plan for Higher Level Experts Aboard ; Major Basic Research Development Program of Shenzhen
WOS记录号WOS:000329130600022
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59253
专题北京大学深圳医院_泌尿外科
作者单位1.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Tumor Clin Immune Gene Therapy Engn Lab, Dept Biobank,Shenzhen Peoples Hosp 2, Shenzhen 518035, Peoples R China
2.Guangdong Med Coll, Affiliated Hosp, Dept Cent Lab, Zhanjiang 524023, Peoples R China
3.Peking Univ, Shenzhen Hosp, Inst Urol, Shenzhen PKU HKUST Med Ctr,Guangdong & Shenzhen K, Shenzhen 518036, Peoples R China
4.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen 518036, Peoples R China
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GB/T 7714
Sun, Xiaojuan,Fa, Pingping,Cui, Zhiwen,et al. The EDA-containing cellular fibronectin induces epithelial-mesenchymal transition in lung cancer cells through integrin alpha 9 beta 1-mediated activation of PI3-K/AKT and Erk1/2 (Retracted article. See vol. 36, pg. 1452, 2015)[J]. CARCINOGENESIS,2014,35(1):184-191.
APA Sun, Xiaojuan.,Fa, Pingping.,Cui, Zhiwen.,Xia, Ye.,Sun, Liang.,...&Cai, Zhiming.(2014).The EDA-containing cellular fibronectin induces epithelial-mesenchymal transition in lung cancer cells through integrin alpha 9 beta 1-mediated activation of PI3-K/AKT and Erk1/2 (Retracted article. See vol. 36, pg. 1452, 2015).CARCINOGENESIS,35(1),184-191.
MLA Sun, Xiaojuan,et al."The EDA-containing cellular fibronectin induces epithelial-mesenchymal transition in lung cancer cells through integrin alpha 9 beta 1-mediated activation of PI3-K/AKT and Erk1/2 (Retracted article. See vol. 36, pg. 1452, 2015)".CARCINOGENESIS 35.1(2014):184-191.
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