北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 化学生物学系  > 期刊论文
学科主题: 药学
题名:
Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis
作者: Liu, Yi2; Zhang, Shuyu1; Ling, Xiaomei1; Li, Yuzhen2; Zhang, Yingmei3,4; Han, Wenling3,4; Wang, Ying3,4
关键词: capillary zone electrophoresis ; CKLF1-C19C ; KLF1-C27 ; heparin ; interaction ; peptide
刊名: JOURNAL OF PEPTIDE SCIENCE
发表日期: 2008-08-01
DOI: 10.1002/psc.1028
卷: 14, 期:8, 页:984-988
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: CHEMOKINE-LIKE FACTOR-1 ; BINDING ; GLYCOSAMINOGLYCANS ; MECHANISM ; TOOL
英文摘要:

The Chemokine-like factor 1 (CKLF1) is a novel human cytokine and exhibits chemotactic activities on leukocytes. Two peptides named CKLF1-C27 and CKLF1-C19. were obtained from secreted CKLF1. In this study. a selective high-performance analytical method based on capillary zone electrophoresis (CZE) to investigate interactions between heparin and CKLF1C27/CKLF1-C19 was developed. Samples containing CKLF1-C27/CKI.F1-C19 and heparin at various ratios were incubated at room temperature and then separated by CZE with Tris-acetate buffer at. pH 7.2. Both qualitative and quantitative characterizations of the binding were determined. The binding constants of the interactions between CKLF1-C27/CKLF1-C19 and heparin were calculated as (3.38 +/- 0.49) x 10(5) M-1 and (1.10 +/- 0.02) x 105 M-1 by Scatchard analysis. To study structural requirements. CKI.FI-C19pm and CKLF1-C19km have been synthesized. and their interactions with heparin have been studied by CZE. We found that the Pro or Lys to Ala substitution within the residues of CKLF1-C19 (CKLF1-C19pm or CKLF1-C19km) strongly decreased or abolished its interaction with heparin. suggesting that the residues of Pro affect: the affinity of CKLF1-C19 for heparin, and the residues of Lys of CKLF1-C19 play the important role for the interaction of CKLF1-C19 and heparin, respectively. The methodology presented should be generally applicable to study peptides and heparin interactions quantitatively and qualitatively. Copyright (C) 2008 European Peptide Society and John Wiley & Sons. Ltd.

语种: 英语
所属项目编号: 2004AA2Z3783 and 2006AA02A305 ; 200257
项目资助者: National High-Tech Research and Development Program of China (863 Program) ; Foundation for the Author of National Excellent Doctoral Dissertation of PR China (FANEDD)
WOS记录号: WOS:000258557700013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59254
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut Anal, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Pharm, Beijing 100044, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Lab Med Immunol, Beijing 100083, Peoples R China
4.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China

Recommended Citation:
Liu, Yi,Zhang, Shuyu,Ling, Xiaomei,et al. Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis[J]. JOURNAL OF PEPTIDE SCIENCE,2008,14(8):984-988.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Yi]'s Articles
[Zhang, Shuyu]'s Articles
[Ling, Xiaomei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Yi]‘s Articles
[Zhang, Shuyu]‘s Articles
[Ling, Xiaomei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace