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学科主题: 药学
题名:
Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis
作者: Liu, Yi2; Zhang, Shuyu1; Ling, Xiaomei1; Li, Yuzhen2; Zhang, Yingmei3,4; Han, Wenling3,4; Wang, Ying3,4
关键词: capillary zone electrophoresis ; CKLF1-C19C ; KLF1-C27 ; heparin ; interaction ; peptide
刊名: JOURNAL OF PEPTIDE SCIENCE
发表日期: 2008-08-01
DOI: 10.1002/psc.1028
卷: 14, 期:8, 页:984-988
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: CHEMOKINE-LIKE FACTOR-1 ; BINDING ; GLYCOSAMINOGLYCANS ; MECHANISM ; TOOL
英文摘要:

The Chemokine-like factor 1 (CKLF1) is a novel human cytokine and exhibits chemotactic activities on leukocytes. Two peptides named CKLF1-C27 and CKLF1-C19. were obtained from secreted CKLF1. In this study. a selective high-performance analytical method based on capillary zone electrophoresis (CZE) to investigate interactions between heparin and CKLF1C27/CKLF1-C19 was developed. Samples containing CKLF1-C27/CKI.F1-C19 and heparin at various ratios were incubated at room temperature and then separated by CZE with Tris-acetate buffer at. pH 7.2. Both qualitative and quantitative characterizations of the binding were determined. The binding constants of the interactions between CKLF1-C27/CKLF1-C19 and heparin were calculated as (3.38 +/- 0.49) x 10(5) M-1 and (1.10 +/- 0.02) x 105 M-1 by Scatchard analysis. To study structural requirements. CKI.FI-C19pm and CKLF1-C19km have been synthesized. and their interactions with heparin have been studied by CZE. We found that the Pro or Lys to Ala substitution within the residues of CKLF1-C19 (CKLF1-C19pm or CKLF1-C19km) strongly decreased or abolished its interaction with heparin. suggesting that the residues of Pro affect: the affinity of CKLF1-C19 for heparin, and the residues of Lys of CKLF1-C19 play the important role for the interaction of CKLF1-C19 and heparin, respectively. The methodology presented should be generally applicable to study peptides and heparin interactions quantitatively and qualitatively. Copyright (C) 2008 European Peptide Society and John Wiley & Sons. Ltd.

语种: 英语
所属项目编号: 2004AA2Z3783 and 2006AA02A305 ; 200257
项目资助者: National High-Tech Research and Development Program of China (863 Program) ; Foundation for the Author of National Excellent Doctoral Dissertation of PR China (FANEDD)
WOS记录号: WOS:000258557700013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59254
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut Anal, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Pharm, Beijing 100044, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Lab Med Immunol, Beijing 100083, Peoples R China
4.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China

Recommended Citation:
Liu, Yi,Zhang, Shuyu,Ling, Xiaomei,et al. Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis[J]. JOURNAL OF PEPTIDE SCIENCE,2008,14(8):984-988.
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