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Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis
Liu, Yi2; Zhang, Shuyu1; Ling, Xiaomei1; Li, Yuzhen2; Zhang, Yingmei3,4; Han, Wenling3,4; Wang, Ying3,4
关键词capillary zone electrophoresis CKLF1-C19C KLF1-C27 heparin interaction peptide
刊名JOURNAL OF PEPTIDE SCIENCE
2008-08-01
DOI10.1002/psc.1028
14期:8页:984-988
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CHEMOKINE-LIKE FACTOR-1 ; BINDING ; GLYCOSAMINOGLYCANS ; MECHANISM ; TOOL
英文摘要

The Chemokine-like factor 1 (CKLF1) is a novel human cytokine and exhibits chemotactic activities on leukocytes. Two peptides named CKLF1-C27 and CKLF1-C19. were obtained from secreted CKLF1. In this study. a selective high-performance analytical method based on capillary zone electrophoresis (CZE) to investigate interactions between heparin and CKLF1C27/CKLF1-C19 was developed. Samples containing CKLF1-C27/CKI.F1-C19 and heparin at various ratios were incubated at room temperature and then separated by CZE with Tris-acetate buffer at. pH 7.2. Both qualitative and quantitative characterizations of the binding were determined. The binding constants of the interactions between CKLF1-C27/CKLF1-C19 and heparin were calculated as (3.38 +/- 0.49) x 10(5) M-1 and (1.10 +/- 0.02) x 105 M-1 by Scatchard analysis. To study structural requirements. CKI.FI-C19pm and CKLF1-C19km have been synthesized. and their interactions with heparin have been studied by CZE. We found that the Pro or Lys to Ala substitution within the residues of CKLF1-C19 (CKLF1-C19pm or CKLF1-C19km) strongly decreased or abolished its interaction with heparin. suggesting that the residues of Pro affect: the affinity of CKLF1-C19 for heparin, and the residues of Lys of CKLF1-C19 play the important role for the interaction of CKLF1-C19 and heparin, respectively. The methodology presented should be generally applicable to study peptides and heparin interactions quantitatively and qualitatively. Copyright (C) 2008 European Peptide Society and John Wiley & Sons. Ltd.

语种英语
WOS记录号WOS:000258557700013
项目编号2004AA2Z3783 and 2006AA02A305 ; 200257
资助机构National High-Tech Research and Development Program of China (863 Program) ; Foundation for the Author of National Excellent Doctoral Dissertation of PR China (FANEDD)
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59254
专题北京大学药学院_化学生物学系
北京大学基础医学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut Anal, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Pharm, Beijing 100044, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Lab Med Immunol, Beijing 100083, Peoples R China
4.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
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GB/T 7714
Liu, Yi,Zhang, Shuyu,Ling, Xiaomei,et al. Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis[J]. JOURNAL OF PEPTIDE SCIENCE,2008,14(8):984-988.
APA Liu, Yi.,Zhang, Shuyu.,Ling, Xiaomei.,Li, Yuzhen.,Zhang, Yingmei.,...&Wang, Ying.(2008).Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis.JOURNAL OF PEPTIDE SCIENCE,14(8),984-988.
MLA Liu, Yi,et al."Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis".JOURNAL OF PEPTIDE SCIENCE 14.8(2008):984-988.
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