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学科主题: 临床医学
题名:
Superior neovascularization and muscle regeneration in ischemic skeletal muscles following VEGF gene transfer by rAAV1 pseudotyped vectors
作者: Yan, H; Guo, YH; Zhang, P; Zu, LY; Dong, XY; Chen, L; Tian, JW; Fan, XL; Wang, NP; Wu, XB; Gao, W
关键词: adeno-associated virus ; muscle ; serotype 1 ; gene delivery ; vascular endothelial growth factor ; ischemia
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2005-10-14
DOI: 10.1016/j.bbrc.2005.08.066
卷: 336, 期:1, 页:287-298
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ; RECOMBINANT ADENOASSOCIATED VIRUS ; CYSTIC-FIBROSIS ; VIRAL VECTORS ; HEMOPHILIA-B ; DOUBLE-BLIND ; FACTOR-IX ; SEROTYPE VECTORS ; PHASE-II ; IN-VIVO
英文摘要:

Recombinant adeno-associated virus serotype 2 (rAAV2) vector has been widely employed for gene therapy. Recent progress suggests that the new serotypes of AAV showed a better performance than did AAV2 in normal tissues. Here, we evaluate the potential role of human vascular endothelial growth factor (VEGF) gene transfer using rAAV vector pseudotyped with serotype I capsid proteins (rAAV1) in the treatment of muscle ischemia. In ischemic skeletal muscles, the rAAV1-LacZ vector allowed higher level, broader distribution, and long-lasting gene expression compared with the rAAV2-LacZ vector. Muscle VEGF165 production following the rAAV1-VEGF165 vector injection was 5-10 times higher than that following the rAAV2-VEGF165 vector injection. VEGF165 production mediated by the rAAV1-VEGF165 vector stimulated a large set of neovascularization with relatively mature vascular structures and enhanced muscle regeneration in the ischemic skeletal muscles. Thus, the rAAV1-NEGF165 vector mediated gene transfer may be a therapeutic approach to peripheral vascular diseases. (c) 2005 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000231941500042
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59263
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.AGTC Gen Technol Co Ltd, Beijing 100176, Peoples R China
2.Lund Univ, Sect Immunol, SE-22184 Lund, Sweden
3.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100083, Peoples R China
4.Peking Univ, Ctr Hlth, Inst Cardiovasc Sci, Beijing 100083, Peoples R China

Recommended Citation:
Yan, H,Guo, YH,Zhang, P,et al. Superior neovascularization and muscle regeneration in ischemic skeletal muscles following VEGF gene transfer by rAAV1 pseudotyped vectors[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,336(1):287-298.
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