IR@PKUHSC  > 北京大学第一临床医学院  > 男科中心
学科主题临床医学
Effect of tankyrase 1 on autophagy in the corpus cavernosum smooth muscle cells from ageing rats with erectile dysfunction and its potential mechanism
Zhang, Jian1; Wu, Xiao-Jun2; Zhuo, De-Xiang3; Liu, Tao1; Li, Wei-Ren1; Mao, Ze-Bin3; Xin, Zhong-Cheng1
关键词ageing autophagy corpora cavernosum corpus cavernosum smooth muscle cells erectile dysfunction senile tankyrase
刊名ASIAN JOURNAL OF ANDROLOGY
2010-09-01
DOI10.1038/aja.2010.44
12期:5页:744-752
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Andrology ; Urology & Nephrology
研究领域[WOS]Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]CONVERTING ENZYME-INHIBITOR ; MAMMALIAN-CELLS ; RECEPTOR ; PHOSPHORYLATION ; ACTIVATION ; EXPRESSION ; COMPLEXES ; DISEASE ; KINASE ; GROWTH
英文摘要

This study compared tankyrase 1 expression and autophagy quantity between erectile dysfunction (ED) and non-ED rats′ corpus cavernosum smooth muscle cells (CSMCs). This study aslo explored the effect and possible mechanism of tankyrase 1 on autophagy and cell proliferation in ageing ED rats′ CSMCs. The intracavernous pressure and mean systemic arterial pressure were measured to investigate erectile function so that eight 24-month-old ED and eight 8-month-old male Wistar rats were choosed respectively. The rat CSMCs were isolated and cultured by enzyme digestion, in which tankyrase 1 expression and autophagy quantity were compared. Tankyrase 1 overexpression was induced with plasmid transfection by Lipofectamine (TM). The effect of tankyrase 1 overexpression on proliferation, autophagy and mTOR pathway in 24-month-old ED rats′ CSMCs was measured by the cell growth curve in MTT assay, cell cycle analysis in flow cytometry (FCM), key protein expression in Western blot, autophagy quantity in transmission electron microscopy, monodansylcadaverine staining and GFP-LC3 fluorescence. The primary CSMCs were confirmed by immunofluorescence, and the purity was 99.1% in FCM. Compared with that of 8-month-old rats, tankyrase 1 expression and autophagy quantity significantly decreased in 24-month-old ED rats′ primary CSMCs (P < 0.01). Tankyrase 1 overexpression significantly increased the growth rate (P < 0.05) and increased the S phase of cell cycle (P < 0.01). The autophagosome quantity was remarkably increased (P < 0.01), LC3-I/II and Beclin 1 were upregulated (P < 0.01 and P < 0.05), and p-p70S6K (Thr(389)) was downregulated in 24-month-old ED rat CSMCs (P < 0.05). In conclusion, Tankyrase 1 and autophagy decrease in the CSMCs from aging rats with ED, and tankyrase 1 may have a positive effect on proliferation by enhancing autophagy and regulating the mTOR signalling pathway.

语种英语
WOS记录号WOS:000281602200016
项目编号30772285 ; Z080507030808011
资助机构National Natural Science Foundation of China ; Beijing Municipal Commission of Science Technology, China
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59267
专题北京大学第一临床医学院_男科中心
北京大学基础医学院
作者单位1.Peking Univ, Androl Ctr, Peking Univ Hosp 1, Beijing 100009, Peoples R China
2.Third Mil Med Univ, Inst Urinary Surg, Southwest Hosp, Chongqing 400038, Peoples R China
3.Peking Univ, Dept Biochem & Mol Biol, Hlth Sci Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Jian,Wu, Xiao-Jun,Zhuo, De-Xiang,et al. Effect of tankyrase 1 on autophagy in the corpus cavernosum smooth muscle cells from ageing rats with erectile dysfunction and its potential mechanism[J]. ASIAN JOURNAL OF ANDROLOGY,2010,12(5):744-752.
APA Zhang, Jian.,Wu, Xiao-Jun.,Zhuo, De-Xiang.,Liu, Tao.,Li, Wei-Ren.,...&Xin, Zhong-Cheng.(2010).Effect of tankyrase 1 on autophagy in the corpus cavernosum smooth muscle cells from ageing rats with erectile dysfunction and its potential mechanism.ASIAN JOURNAL OF ANDROLOGY,12(5),744-752.
MLA Zhang, Jian,et al."Effect of tankyrase 1 on autophagy in the corpus cavernosum smooth muscle cells from ageing rats with erectile dysfunction and its potential mechanism".ASIAN JOURNAL OF ANDROLOGY 12.5(2010):744-752.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Jian]的文章
[Wu, Xiao-Jun]的文章
[Zhuo, De-Xiang]的文章
百度学术
百度学术中相似的文章
[Zhang, Jian]的文章
[Wu, Xiao-Jun]的文章
[Zhuo, De-Xiang]的文章
必应学术
必应学术中相似的文章
[Zhang, Jian]的文章
[Wu, Xiao-Jun]的文章
[Zhuo, De-Xiang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。