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学科主题: 临床医学
题名:
Effect of tankyrase 1 on autophagy in the corpus cavernosum smooth muscle cells from ageing rats with erectile dysfunction and its potential mechanism
作者: Zhang, Jian1; Wu, Xiao-Jun2; Zhuo, De-Xiang3; Liu, Tao1; Li, Wei-Ren1; Mao, Ze-Bin3; Xin, Zhong-Cheng1
关键词: ageing ; autophagy ; corpora cavernosum ; corpus cavernosum smooth muscle cells ; erectile dysfunction ; senile ; tankyrase
刊名: ASIAN JOURNAL OF ANDROLOGY
发表日期: 2010-09-01
DOI: 10.1038/aja.2010.44
卷: 12, 期:5, 页:744-752
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Andrology ; Urology & Nephrology
研究领域[WOS]: Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]: CONVERTING ENZYME-INHIBITOR ; MAMMALIAN-CELLS ; RECEPTOR ; PHOSPHORYLATION ; ACTIVATION ; EXPRESSION ; COMPLEXES ; DISEASE ; KINASE ; GROWTH
英文摘要:

This study compared tankyrase 1 expression and autophagy quantity between erectile dysfunction (ED) and non-ED rats′ corpus cavernosum smooth muscle cells (CSMCs). This study aslo explored the effect and possible mechanism of tankyrase 1 on autophagy and cell proliferation in ageing ED rats′ CSMCs. The intracavernous pressure and mean systemic arterial pressure were measured to investigate erectile function so that eight 24-month-old ED and eight 8-month-old male Wistar rats were choosed respectively. The rat CSMCs were isolated and cultured by enzyme digestion, in which tankyrase 1 expression and autophagy quantity were compared. Tankyrase 1 overexpression was induced with plasmid transfection by Lipofectamine (TM). The effect of tankyrase 1 overexpression on proliferation, autophagy and mTOR pathway in 24-month-old ED rats′ CSMCs was measured by the cell growth curve in MTT assay, cell cycle analysis in flow cytometry (FCM), key protein expression in Western blot, autophagy quantity in transmission electron microscopy, monodansylcadaverine staining and GFP-LC3 fluorescence. The primary CSMCs were confirmed by immunofluorescence, and the purity was 99.1% in FCM. Compared with that of 8-month-old rats, tankyrase 1 expression and autophagy quantity significantly decreased in 24-month-old ED rats′ primary CSMCs (P < 0.01). Tankyrase 1 overexpression significantly increased the growth rate (P < 0.05) and increased the S phase of cell cycle (P < 0.01). The autophagosome quantity was remarkably increased (P < 0.01), LC3-I/II and Beclin 1 were upregulated (P < 0.01 and P < 0.05), and p-p70S6K (Thr(389)) was downregulated in 24-month-old ED rat CSMCs (P < 0.05). In conclusion, Tankyrase 1 and autophagy decrease in the CSMCs from aging rats with ED, and tankyrase 1 may have a positive effect on proliferation by enhancing autophagy and regulating the mTOR signalling pathway.

语种: 英语
所属项目编号: 30772285 ; Z080507030808011
项目资助者: National Natural Science Foundation of China ; Beijing Municipal Commission of Science Technology, China
WOS记录号: WOS:000281602200016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59267
Appears in Collections:北京大学第一临床医学院_男科中心_期刊论文

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作者单位: 1.Peking Univ, Androl Ctr, Peking Univ Hosp 1, Beijing 100009, Peoples R China
2.Third Mil Med Univ, Inst Urinary Surg, Southwest Hosp, Chongqing 400038, Peoples R China
3.Peking Univ, Dept Biochem & Mol Biol, Hlth Sci Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Jian,Wu, Xiao-Jun,Zhuo, De-Xiang,et al. Effect of tankyrase 1 on autophagy in the corpus cavernosum smooth muscle cells from ageing rats with erectile dysfunction and its potential mechanism[J]. ASIAN JOURNAL OF ANDROLOGY,2010,12(5):744-752.
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