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学科主题基础医学
A type-specific nested PCR assay established and applied for investigation of HBV genotype and subgenotype in Chinese patients with chronic HBV infection
Nie, Jing-Jing; Sun, Kui-Xia; Li, Jie; Wang, Jie; Jin, Hui; Wang, Ling; Lu, Feng-Min; Li, Tong; Yan, Ling; Yang, Jing-Xian; Sun, Mi-Shu; Zhuang, Hui
关键词Genotype Hepatitis B virus Nested PCR Subgenotype Type-specific nested PCR
刊名VIROLOGY JOURNAL
2012-06-19
DOI10.1186/1743-422X-9-121
9期:1
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Virology
研究领域[WOS]Virology
关键词[WOS]HEPATITIS-B-VIRUS ; LENGTH-POLYMORPHISM PATTERNS ; POLYMERASE-CHAIN-REACTION ; GENETIC-VARIABILITY ; SURFACE-ANTIGEN ; MULTIPLEX-PCR ; A-F ; SYSTEM ; DNA ; MUTATION
英文摘要

Background: Many studies have suggested that hepatitis B virus (HBV) genotypes show not only geographical distribution and race specificity, but also are associated with disease progression and response to interferon treatment. The objective of this study was to develop a nested polymerase chain reaction (nPCR) assay for genotypes A-D and subgenotypes B1, B2, C1 and C2 of hepatitis B virus (HBV) and to investigate the distribution characteristics of HBV genotypes/subgenotype in China.

Methods: After redesigning the primers and optimizing the reaction conditions using common Taq polymerase, the sensitivity, specificity and reproducibility of the method were evaluated using plasmids and serum samples. In total, 642 serum samples from patients with chronic HBV infection were applied to investigate the distribution of HBV genotype and subgenotype in China.

Results: The genotype and subgenotype could be identified when the HBV DNA load of a sample was >= 10(2.3) IU/mL. For the 639 successfully genotyped samples, the sequencing results of 130 randomly selected samples (20.3%, 130/639) were consistent with those of the nPCR method. The present study showed that HBV genotype B (11.2%, 72/642), C (68.2%, 438/642) and D (7.2%, 46/642) were circulating in China, while genotype C was the dominant strain except for western region where genotype D was the prevalent strain. The main subgenotypes of genotypes B and C were B2 (87.5%, 63/72) and C2 (92.9%, 407/438), respectively.

Conclusions: The low-cost nPCR method would be a useful tool for clinical and epidemiological investigation in the regions where genotypes A-D are predominant.

语种英语
WOS记录号WOS:000310005400001
项目编号2008ZX10002-001 ; 2008ZX10002-012
资助机构Major Science and Technology Special Project of the Eleventh Five-year Plan of China
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59269
专题北京大学基础医学院_病原生物学系
北京大学基础医学院
北京大学第一临床医学院_口腔科
北京大学临床肿瘤学院_肿瘤放疗科
作者单位Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Microbiol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Nie, Jing-Jing,Sun, Kui-Xia,Li, Jie,et al. A type-specific nested PCR assay established and applied for investigation of HBV genotype and subgenotype in Chinese patients with chronic HBV infection[J]. VIROLOGY JOURNAL,2012,9(1).
APA Nie, Jing-Jing.,Sun, Kui-Xia.,Li, Jie.,Wang, Jie.,Jin, Hui.,...&Zhuang, Hui.(2012).A type-specific nested PCR assay established and applied for investigation of HBV genotype and subgenotype in Chinese patients with chronic HBV infection.VIROLOGY JOURNAL,9(1).
MLA Nie, Jing-Jing,et al."A type-specific nested PCR assay established and applied for investigation of HBV genotype and subgenotype in Chinese patients with chronic HBV infection".VIROLOGY JOURNAL 9.1(2012).
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