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学科主题: 基础医学
题名:
A type-specific nested PCR assay established and applied for investigation of HBV genotype and subgenotype in Chinese patients with chronic HBV infection
作者: Nie, Jing-Jing; Sun, Kui-Xia; Li, Jie; Wang, Jie; Jin, Hui; Wang, Ling; Lu, Feng-Min; Li, Tong; Yan, Ling; Yang, Jing-Xian; Sun, Mi-Shu; Zhuang, Hui
关键词: Genotype ; Hepatitis B virus ; Nested PCR ; Subgenotype ; Type-specific nested PCR
刊名: VIROLOGY JOURNAL
发表日期: 2012-06-19
DOI: 10.1186/1743-422X-9-121
卷: 9, 期:1
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Virology
研究领域[WOS]: Virology
关键词[WOS]: HEPATITIS-B-VIRUS ; LENGTH-POLYMORPHISM PATTERNS ; POLYMERASE-CHAIN-REACTION ; GENETIC-VARIABILITY ; SURFACE-ANTIGEN ; MULTIPLEX-PCR ; A-F ; SYSTEM ; DNA ; MUTATION
英文摘要:

Background: Many studies have suggested that hepatitis B virus (HBV) genotypes show not only geographical distribution and race specificity, but also are associated with disease progression and response to interferon treatment. The objective of this study was to develop a nested polymerase chain reaction (nPCR) assay for genotypes A-D and subgenotypes B1, B2, C1 and C2 of hepatitis B virus (HBV) and to investigate the distribution characteristics of HBV genotypes/subgenotype in China.

Methods: After redesigning the primers and optimizing the reaction conditions using common Taq polymerase, the sensitivity, specificity and reproducibility of the method were evaluated using plasmids and serum samples. In total, 642 serum samples from patients with chronic HBV infection were applied to investigate the distribution of HBV genotype and subgenotype in China.

Results: The genotype and subgenotype could be identified when the HBV DNA load of a sample was >= 10(2.3) IU/mL. For the 639 successfully genotyped samples, the sequencing results of 130 randomly selected samples (20.3%, 130/639) were consistent with those of the nPCR method. The present study showed that HBV genotype B (11.2%, 72/642), C (68.2%, 438/642) and D (7.2%, 46/642) were circulating in China, while genotype C was the dominant strain except for western region where genotype D was the prevalent strain. The main subgenotypes of genotypes B and C were B2 (87.5%, 63/72) and C2 (92.9%, 407/438), respectively.

Conclusions: The low-cost nPCR method would be a useful tool for clinical and epidemiological investigation in the regions where genotypes A-D are predominant.

语种: 英语
所属项目编号: 2008ZX10002-001 ; 2008ZX10002-012
项目资助者: Major Science and Technology Special Project of the Eleventh Five-year Plan of China
WOS记录号: WOS:000310005400001
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59269
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Microbiol, Beijing 100191, Peoples R China

Recommended Citation:
Nie, Jing-Jing,Sun, Kui-Xia,Li, Jie,et al. A type-specific nested PCR assay established and applied for investigation of HBV genotype and subgenotype in Chinese patients with chronic HBV infection[J]. VIROLOGY JOURNAL,2012,9(1).
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