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Structural Characterization of a Recombinant Fusion Protein by Instrumental Analysis and Molecular Modeling
Wu, Zhigang1; Zhou, Peng2; Li, Xiaoxin3; Wang, Hui4; Luo, Delun1; Qiao, Huaiyao1; Ke, Xiao1; Huang, Jian2
刊名PLOS ONE
2013-03-04
DOI10.1371/journal.pone.0057642
8期:3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]ENDOTHELIAL GROWTH-FACTOR ; ELECTROSPRAY MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; MONOCLONAL-ANTIBODY ; SAMPLE PREPARATION ; DEAMIDATION SITES ; MEMBRANE-PROTEIN ; IDENTIFICATION ; RECEPTOR ; NEOVASCULARIZATION
英文摘要

Conbercept is a genetically engineered homodimeric protein for the treatment of wet age-related macular degeneration (wet AMD) that functions by blocking VEGF-family proteins. Its huge, highly variable architecture makes characterization and development of a functional assay difficult. In this study, the primary structure, number of disulfide linkages and glycosylation state of conbercept were characterized by high-performance liquid chromatography, mass spectrometry, and capillary electrophoresis. Molecular modeling was then applied to obtain the spatial structural model of the conbercept-VEGF-A complex, and to study its inter-atomic interactions and dynamic behavior. This work was incorporated into a platform useful for studying the structure of conbercept and its ligand binding functions.

语种英语
WOS记录号WOS:000315634900032
项目编号2011CB510200 ; 61071177 ; NCET-12-0088
资助机构973 projects ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59282
专题北京大学第二临床医学院
北京大学药学院_化学生物学系
作者单位1.Chengdu Kanghong Biotechnol Inc, Chengdu, Peoples R China
2.Peking Univ, Peoples Hosp, Beijing 100871, Peoples R China
3.Univ Elect Sci & Technol China, Ctr Bioinformat, Chengdu 610054, Peoples R China
4.Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogens & Biosecur, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Wu, Zhigang,Zhou, Peng,Li, Xiaoxin,et al. Structural Characterization of a Recombinant Fusion Protein by Instrumental Analysis and Molecular Modeling[J]. PLOS ONE,2013,8(3).
APA Wu, Zhigang.,Zhou, Peng.,Li, Xiaoxin.,Wang, Hui.,Luo, Delun.,...&Huang, Jian.(2013).Structural Characterization of a Recombinant Fusion Protein by Instrumental Analysis and Molecular Modeling.PLOS ONE,8(3).
MLA Wu, Zhigang,et al."Structural Characterization of a Recombinant Fusion Protein by Instrumental Analysis and Molecular Modeling".PLOS ONE 8.3(2013).
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