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An integrated system for identifying the hidden assassins in traditional medicines containing aristolochic acids
Wu, Lan1,2; Sun, Wei1; Wang, Bo3,4; Zhao, Haiyu1; Li, Yaoli5; Cai, Shaoqing5; Xiang, Li1; Zhu, Yingjie1; Yao, Hui3,4; Song, Jingyuan3,4; Cheng, Yung-Chi6; Chen, Shilin1
刊名SCIENTIFIC REPORTS
2015-08-13
DOI10.1038/srep11318
5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]INTERNAL TRANSCRIBED SPACER ; CHINESE HERBS NEPHROPATHY ; PLANT DNA BARCODES ; REAL-TIME PCR ; SPECIES IDENTIFICATION ; LAND PLANTS ; AUTHENTICATION ; PRODUCTS ; CONTAMINATION ; REGION
英文摘要

Traditional herbal medicines adulterated and contaminated with plant materials from the Aristolochiaceae family, which contain aristolochic acids (AAs), cause aristolochic acid nephropathy. Approximately 256 traditional Chinese patent medicines, containing Aristolochiaceous materials, are still being sold in Chinese markets today. In order to protect consumers from health risks due to AAs, the hidden assassins, efficient methods to differentiate Aristolochiaceous herbs from their putative substitutes need to be established. In this study, 158 Aristolochiaceous samples representing 46 species and four genera as well as 131 non-Aristolochiaceous samples representing 33 species, 20 genera and 12 families were analyzed using DNA barcodes based on the ITS2 and psbA-trnH sequences. Aristolochiaceous materials and their non-Aristolochiaceous substitutes were successfully identified using BLAST1, the nearest distance method and the neighbor-joining (NJ) tree. In addition, based on sequence information of ITS2, we developed a Real-Time PCR assay which successfully identified herbal material from the Aristolochiaceae family. Using Ultra High Performance Liquid Chromatography-Mass Spectrometer (UHPLC-HR-MS), we demonstrated that most representatives from the Aristolochiaceae family contain toxic AAs. Therefore, integrated DNA barcodes, Real-Time PCR assays using TaqMan probes and UHPLC-HR-MS system provides an efficient and reliable authentication system to protect consumers from health risks due to the hidden assassins (AAs).

语种英语
WOS记录号WOS:000359406900001
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59300
专题北京大学药学院
作者单位1.Hubei Univ Chinese Med, Coll Pharm, Wuhan, Peoples R China
2.Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06510 USA
3.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing, Peoples R China
4.Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100730, Peoples R China
5.Peking Union Med Coll, Beijing 100021, Peoples R China
6.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Wu, Lan,Sun, Wei,Wang, Bo,et al. An integrated system for identifying the hidden assassins in traditional medicines containing aristolochic acids[J]. SCIENTIFIC REPORTS,2015,5.
APA Wu, Lan.,Sun, Wei.,Wang, Bo.,Zhao, Haiyu.,Li, Yaoli.,...&Chen, Shilin.(2015).An integrated system for identifying the hidden assassins in traditional medicines containing aristolochic acids.SCIENTIFIC REPORTS,5.
MLA Wu, Lan,et al."An integrated system for identifying the hidden assassins in traditional medicines containing aristolochic acids".SCIENTIFIC REPORTS 5(2015).
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