北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 化学生物学系  > 期刊论文
学科主题: 药学
题名:
Cholestane-3 beta,5 alpha,6 beta-triol promotes vascular smooth muscle cells calcification
作者: Liu, HM; Yuan, L; Xu, SJ; Zhang, TL; Wang, K
关键词: cholestane-3 beta ; 5 alpha ; 6 beta-triol ; calcification ; vascular smooth muscle cells ; reactive oxygen species
刊名: LIFE SCIENCES
发表日期: 2004-12-17
DOI: 10.1016/j.lfs.2004.06.025
卷: 76, 期:5, 页:533-543
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: LOW-DENSITY-LIPOPROTEIN ; ATHEROSCLEROTIC PLAQUES ; ARTERIAL CALCIFICATION ; ENDOTHELIAL-CELLS ; APOPTOSIS ; OXYSTEROLS ; MACROPHAGES ; 7-BETA-HYDROXYCHOLESTEROL ; OSTEOPONTIN ; ACTIVATION
英文摘要:

Oxysterols found in atherosclerotic plaque may be associated with vascular calcification. We investigated the effect of oxysterol cholestane-3beta, 5alpha, 6beta-triol (Triol) on in vitro calcification of rat vascular smooth muscle cells (VSMCs). In vitro calcification was induced by incubation of VSMCs with beta-glycerophosphate. Calcifying nodule formation, calcium deposition in extracellular matrix, and alkaline phosphatase (ALP) activity were measured as indices of calcification. Because apoptotic bodies can serve as nucleation sites for calcification, apoptosis of calcifying VSMCs was determined by Hoechst 33258 staining, TUNEL, and FITC-labeled annexin V/PI double staining. The calcium deposition and ALP activity in calcifying VSMCs were much higher than those in non-calcifying VSMCs. Triol increased calcifying nodule formation, calcium deposition, ALP activity, and apoptosis of nodular cells in calcifying VSMCs. As determined by 2,7-dichlorofluorescein fluorescence, Triol induced the generation of reactive oxygen species (ROS) in calcifying VSMCs dose- and time-dependently. Triol-induced increases in calcium deposition, ALP activity, apoptosis, and ROS generation were all attenuated by antioxidant vitamin C plus vitamin E (VC + VE). The results demonstrated that Triol promoted VSMCs calcification through direct increase of ALP activity and apoptosis, probably by ROS-related mechanism. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000225454700006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59304
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, Med & Hlth Anal Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Liu, HM,Yuan, L,Xu, SJ,et al. Cholestane-3 beta,5 alpha,6 beta-triol promotes vascular smooth muscle cells calcification[J]. LIFE SCIENCES,2004,76(5):533-543.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, HM]'s Articles
[Yuan, L]'s Articles
[Xu, SJ]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, HM]‘s Articles
[Yuan, L]‘s Articles
[Xu, SJ]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace