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Cholestane-3 beta,5 alpha,6 beta-triol promotes vascular smooth muscle cells calcification
Liu, HM; Yuan, L; Xu, SJ; Zhang, TL; Wang, K
关键词cholestane-3 beta 5 alpha 6 beta-triol calcification vascular smooth muscle cells reactive oxygen species
刊名LIFE SCIENCES
2004-12-17
DOI10.1016/j.lfs.2004.06.025
76期:5页:533-543
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]LOW-DENSITY-LIPOPROTEIN ; ATHEROSCLEROTIC PLAQUES ; ARTERIAL CALCIFICATION ; ENDOTHELIAL-CELLS ; APOPTOSIS ; OXYSTEROLS ; MACROPHAGES ; 7-BETA-HYDROXYCHOLESTEROL ; OSTEOPONTIN ; ACTIVATION
英文摘要

Oxysterols found in atherosclerotic plaque may be associated with vascular calcification. We investigated the effect of oxysterol cholestane-3beta, 5alpha, 6beta-triol (Triol) on in vitro calcification of rat vascular smooth muscle cells (VSMCs). In vitro calcification was induced by incubation of VSMCs with beta-glycerophosphate. Calcifying nodule formation, calcium deposition in extracellular matrix, and alkaline phosphatase (ALP) activity were measured as indices of calcification. Because apoptotic bodies can serve as nucleation sites for calcification, apoptosis of calcifying VSMCs was determined by Hoechst 33258 staining, TUNEL, and FITC-labeled annexin V/PI double staining. The calcium deposition and ALP activity in calcifying VSMCs were much higher than those in non-calcifying VSMCs. Triol increased calcifying nodule formation, calcium deposition, ALP activity, and apoptosis of nodular cells in calcifying VSMCs. As determined by 2,7-dichlorofluorescein fluorescence, Triol induced the generation of reactive oxygen species (ROS) in calcifying VSMCs dose- and time-dependently. Triol-induced increases in calcium deposition, ALP activity, apoptosis, and ROS generation were all attenuated by antioxidant vitamin C plus vitamin E (VC + VE). The results demonstrated that Triol promoted VSMCs calcification through direct increase of ALP activity and apoptosis, probably by ROS-related mechanism. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000225454700006
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59304
专题北京大学药学院_化学生物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, Med & Hlth Anal Ctr, Beijing 100083, Peoples R China
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Liu, HM,Yuan, L,Xu, SJ,et al. Cholestane-3 beta,5 alpha,6 beta-triol promotes vascular smooth muscle cells calcification[J]. LIFE SCIENCES,2004,76(5):533-543.
APA Liu, HM,Yuan, L,Xu, SJ,Zhang, TL,&Wang, K.(2004).Cholestane-3 beta,5 alpha,6 beta-triol promotes vascular smooth muscle cells calcification.LIFE SCIENCES,76(5),533-543.
MLA Liu, HM,et al."Cholestane-3 beta,5 alpha,6 beta-triol promotes vascular smooth muscle cells calcification".LIFE SCIENCES 76.5(2004):533-543.
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