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学科主题: 基础医学
题名:
Simultaneous determination of biotoxins DSP and AZAs in bivalve molluscs and fish by liquid chromatography/tandem mass spectrometry
作者: Zhuo, Liyang1,2; Fu, Wusheng3; Yang, Yiqiang2; Qiu, Bin1; Lin, Zhenyu1; Shan, Liyang7; Zheng, Lemin4,5,6; Li, Jianrong8; Chen, Guonan1
刊名: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
发表日期: 2014-07-15
DOI: 10.1002/rcm.6900
卷: 28, 期:13, 页:1479-1488
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
关键词[WOS]: SHELLFISH TOXINS ; MUSSELS ; AZASPIRACIDS ; MS
英文摘要:

RATIONALEA method has been developed for simultaneous determination of the toxins OA, DTX-1, AZA-1, AZA-2 and AZA-3 in various aquatic products as these can cause diarrhoetic shellfish poisoning (DSP) in humans, an intoxication characterized by vomiting and diarrhea. METHODSSeparation of the toxins was achieved on a C18 column (150mmx2.1mm, 3.5 mu m) using an acetonitrile/water gradient with formic acid as an eluent modifier. Electrospray ionisation (ESI) in negative mode was used to generate the molecule related ion [M-H]-, for OA and DTX-1, while ESI in positive mode was used to generate the molecule related ion [M+H]+ for AZAs. Samples were extracted with 80% methanol, followed by partitioning with ethyl acetate, purified on a Poly-Sery MAX cartridge and finally analyzed by LC/ESI-MS/MS in the multiple reaction monitoring (MRM) mode. RESULTSThe limit of detection (LOD) and limit of qualification (LOQ) of the method were in the range of 0.02-0.79 mu g/kg and 0.07-2.64 mu g/kg in Scomberomorus niphonius, blood clam and oyster, respectively, recoveries of the toxins at three fortification levels ranged from 71.3% to 104.8% with relative standard deviation from 1.0% to 12.5%. The calibration curves were well linear between the LC peak area of the selected ion pair and the concentration of the toxins, with the correlation coefficient over 0.99. CONCLUSIONSThe method was sufficiently sensitive to permit the determination of the toxins DSP and AZA in sea food. Copyright (c) 2014 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 2010QK024 ; FJQI2009005 ; 2012BAD29B06 ; 2011N5008
项目资助者: scientific research project of General Administration of Quality Supervision, Inspection and Quarantine of China ; scientific research project of Fujian Provincial Bureau of Quality and Technical Supervision ; National Key Technologies R &amp ; D Program of China during the 12th Five-Year Plan Period ; Major Project of Fujian Province
WOS记录号: WOS:000337532300009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59307
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: 1.Fuzhou Univ, Key Lab Anal & Detect Technol Food Safety, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350002, Peoples R China
2.Qual Inspect Inst Quanzhou, Quanzhou 362000, Fujian, Peoples R China
3.Fujian Ctr Dis Control & Prevent, Fuzhou 350001, Fujian, Peoples R China
4.Peking Univ, Key Lab Mol Cardiovasc Sci, Sch Basic Med Sci, Hlth Sci Ctr,Educ Minist,Inst Cardiovasc Sci, Beijing 100191, Peoples R China
5.Peking Univ, Key Lab Mol Cardiovasc Sci, Sch Basic Med Sci, Inst Syst Biomed,Hlth Sci Ctr,Educ Minist, Beijing 100191, Peoples R China
6.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Beijing 100191, Peoples R China
7.Nanjing Med Univ, Lab Human Funct, Atherosclerosis Res Ctr, Nanjing 210029, Jiangsu, Peoples R China
8.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Food Safety Key Lab Zhejiang Prov, Hangzhou 310035, Zhejiang, Peoples R China

Recommended Citation:
Zhuo, Liyang,Fu, Wusheng,Yang, Yiqiang,et al. Simultaneous determination of biotoxins DSP and AZAs in bivalve molluscs and fish by liquid chromatography/tandem mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2014,28(13):1479-1488.
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