IR@PKUHSC  > 北京大学第三临床医学院  > 生殖医学中心
学科主题临床医学
Integration of single oocyte trapping, in vitro fertilization and embryo culture in a microwell-structured microfluidic device
Han, Chao1,2; Zhang, Qiufang3; Ma, Rui1,2; Xie, Lan1,2; Qiu, Tian1,2; Wang, Lei2; Mitchelson, Keith1,2; Wang, Jundong4; Huang, Guoliang1,2; Qiao, Jie3; Cheng, Jing1,2
刊名LAB ON A CHIP
2010
DOI10.1039/c005296e
10期:21页:2848-2854
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
资助者National High-Tech Program ; National High-Tech Program
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
关键词[WOS]CELL-CULTURE ; SPERM ; POLY(DIMETHYLSILOXANE) ; CHANNELS ; SYSTEM ; MOUSE ; IVF
英文摘要

In vitro fertilization (IVF) therapy is an important treatment for human infertility. However, the methods for clinical IVF have only changed slightly over decades: culture medium is held in oil-covered drops in Petri dishes and manipulation occurs by manual pipetting. Here we report a novel microwell-structured microfluidic device that integrates single oocyte trapping, fertilization and subsequent embryo culture. A microwell array was used to capture and hold individual oocytes during the flow-through process of oocyte and sperm loading, medium substitution and debris cleaning. Different microwell depths were compared by computational modeling and flow washing experiments for their effectiveness in oocyte trapping and debris removal. Fertilization was achieved in the microfluidic devices with similar fertilization rates to standard oil-covered drops in Petri dishes. Embryos could be cultured to blastocyst stages in our devices with developmental status individually monitored and tracked. The results suggest that the microfluidic device may bring several advantages to IVF practices by simplifying oocyte handling and manipulation, allowing rapid and convenient medium changing, and enabling automated tracking of any single embryo development.

语种英语
所属项目编号2006AA020701
资助者National High-Tech Program ; National High-Tech Program
WOS记录号WOS:000282808700004
引用统计
被引频次:49[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59310
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Tsinghua Univ, Dept Biomed Engn, Sch Med, Beijing 100084, Peoples R China
2.Natl Engn Res Ctr Beijing Biochip Technol, Beijing 102206, Peoples R China
3.Peking Univ, Ctr Reprod Med, Dept Obstet & Gynecol, Hosp 3, Beijing 100191, Peoples R China
4.Shanxi Agr Univ, Shanxi Key Lab Ecol Anim Sci & Environm Med, Taigu 030801, Shanxi, Peoples R China
推荐引用方式
GB/T 7714
Han, Chao,Zhang, Qiufang,Ma, Rui,et al. Integration of single oocyte trapping, in vitro fertilization and embryo culture in a microwell-structured microfluidic device[J]. LAB ON A CHIP,2010,10(21):2848-2854.
APA Han, Chao.,Zhang, Qiufang.,Ma, Rui.,Xie, Lan.,Qiu, Tian.,...&Cheng, Jing.(2010).Integration of single oocyte trapping, in vitro fertilization and embryo culture in a microwell-structured microfluidic device.LAB ON A CHIP,10(21),2848-2854.
MLA Han, Chao,et al."Integration of single oocyte trapping, in vitro fertilization and embryo culture in a microwell-structured microfluidic device".LAB ON A CHIP 10.21(2010):2848-2854.
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