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学科主题: 临床医学
题名:
Integration of single oocyte trapping, in vitro fertilization and embryo culture in a microwell-structured microfluidic device
作者: Han, Chao1,2; Zhang, Qiufang3; Ma, Rui1,2; Xie, Lan1,2; Qiu, Tian1,2; Wang, Lei2; Mitchelson, Keith1,2; Wang, Jundong4; Huang, Guoliang1,2; Qiao, Jie3; Cheng, Jing1,2
刊名: LAB ON A CHIP
发表日期: 2010
DOI: 10.1039/c005296e
卷: 10, 期:21, 页:2848-2854
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
关键词[WOS]: CELL-CULTURE ; SPERM ; POLY(DIMETHYLSILOXANE) ; CHANNELS ; SYSTEM ; MOUSE ; IVF
英文摘要:

In vitro fertilization (IVF) therapy is an important treatment for human infertility. However, the methods for clinical IVF have only changed slightly over decades: culture medium is held in oil-covered drops in Petri dishes and manipulation occurs by manual pipetting. Here we report a novel microwell-structured microfluidic device that integrates single oocyte trapping, fertilization and subsequent embryo culture. A microwell array was used to capture and hold individual oocytes during the flow-through process of oocyte and sperm loading, medium substitution and debris cleaning. Different microwell depths were compared by computational modeling and flow washing experiments for their effectiveness in oocyte trapping and debris removal. Fertilization was achieved in the microfluidic devices with similar fertilization rates to standard oil-covered drops in Petri dishes. Embryos could be cultured to blastocyst stages in our devices with developmental status individually monitored and tracked. The results suggest that the microfluidic device may bring several advantages to IVF practices by simplifying oocyte handling and manipulation, allowing rapid and convenient medium changing, and enabling automated tracking of any single embryo development.

语种: 英语
所属项目编号: 2006AA020701
项目资助者: National High-Tech Program
WOS记录号: WOS:000282808700004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59310
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Tsinghua Univ, Dept Biomed Engn, Sch Med, Beijing 100084, Peoples R China
2.Natl Engn Res Ctr Beijing Biochip Technol, Beijing 102206, Peoples R China
3.Peking Univ, Ctr Reprod Med, Dept Obstet & Gynecol, Hosp 3, Beijing 100191, Peoples R China
4.Shanxi Agr Univ, Shanxi Key Lab Ecol Anim Sci & Environm Med, Taigu 030801, Shanxi, Peoples R China

Recommended Citation:
Han, Chao,Zhang, Qiufang,Ma, Rui,et al. Integration of single oocyte trapping, in vitro fertilization and embryo culture in a microwell-structured microfluidic device[J]. LAB ON A CHIP,2010,10(21):2848-2854.
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