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A proteomic approach for plasma biomarker discovery with 8-plex iTRAQ labeling and SCX-LC-MS/MS
Ye, Haige1; Sun, Li2; Huang, Xiaojun1; Zhang, Ping2; Zhao, Xiaoshu1
关键词Plasma protein Fractionation 8-plex iTRAQ SCX-LC-MS/MS
刊名MOLECULAR AND CELLULAR BIOCHEMISTRY
2010-10-01
DOI10.1007/s11010-010-0502-x
343期:1-2页:91-99
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
资助者National Outstanding Young Scientist&prime ; s Foundation of China ; National Outstanding Young Scientist&prime ; s Foundation of China
研究领域[WOS]Cell Biology
关键词[WOS]MASS-SPECTROMETRY ; TOF-MS ; PROTEINS ; SERUM ; DISEASE ; PROJECT ; FRACTIONATION ; TECHNOLOGY ; ANTIBODIES ; PROTOCOL
英文摘要

Plasma is recognized as a promising source of disease-related biomarkers, and proteomic approaches for identifying novel plasma biomarkers are in great demand. However, the complexity and dynamic protein concentration range of plasma remain the main obstacles for current research in this field. In this study, plasma proteins were prefractioned by immunodepletion and Protein Equalizer Technology to remove high abundant proteins, then labeled with an 8-plex isobaric tags for relative and absolute quantitation (iTRAQ) to improve the peptide ionization, and analyzed by strong-cation-exchange(SCX) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Our results showed that both prefraction methods were complementary, with regard to the number of identified proteins. Good chromatographic technique is important to further fractionate the iTRAQ labeling peptides, which allowed 320 and 248 different proteins to be characterized from two prefraction methods, respectively, encompassing a wide array of biological functions and a broad dynamic range of 10(7). Furthermore, the accuracy of iTRAQ relative quantitation for differentially expressed proteins is associated with the number of peptides hits per protein.

语种英语
所属项目编号30725038
资助者National Outstanding Young Scientist&prime ; s Foundation of China ; National Outstanding Young Scientist&prime ; s Foundation of China
WOS记录号WOS:000282050300011
引用统计
被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59324
专题北京大学第二临床医学院_血液科
作者单位1.Peking Univ, Peoples Hosp, Inst Hematol, Beijing 100044, Peoples R China
2.Biosteed Gene Express Tech Co Ltd, Xiamen 361022, Fujian, Peoples R China
推荐引用方式
GB/T 7714
Ye, Haige,Sun, Li,Huang, Xiaojun,et al. A proteomic approach for plasma biomarker discovery with 8-plex iTRAQ labeling and SCX-LC-MS/MS[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2010,343(1-2):91-99.
APA Ye, Haige,Sun, Li,Huang, Xiaojun,Zhang, Ping,&Zhao, Xiaoshu.(2010).A proteomic approach for plasma biomarker discovery with 8-plex iTRAQ labeling and SCX-LC-MS/MS.MOLECULAR AND CELLULAR BIOCHEMISTRY,343(1-2),91-99.
MLA Ye, Haige,et al."A proteomic approach for plasma biomarker discovery with 8-plex iTRAQ labeling and SCX-LC-MS/MS".MOLECULAR AND CELLULAR BIOCHEMISTRY 343.1-2(2010):91-99.
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