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学科主题临床医学
Internalization and distribution of three alpha(1)-adrenoceptor subtypes in HEK293A cells before and after agonist stimulation
Wang, Shu-yi; Song, Yao; Xu, Ming; He, Qi-hua; Han, Qi-de; Zhang, You-yi
关键词adrenoceptor agonist stimulation confocal image enzyme-linked immunosorbent assay [H-3]-prazosin binding assay internalization subcellular distribution subtype
刊名ACTA PHARMACOLOGICA SINICA
2007-03-01
DOI10.1111/j.1745-7254-2007-00509-x
28期:3页:359-366
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]PROTEIN-COUPLED RECEPTORS ; ALPHA(1B)-ADRENERGIC RECEPTOR ; ALPHA(1D)-ADRENERGIC RECEPTORS ; SUBCELLULAR-LOCALIZATION ; MEDIATED INTERNALIZATION ; CELLULAR-LOCALIZATION ; SMOOTH-MUSCLE ; DESENSITIZATION ; PHOSPHORYLATION ; EXPRESSION
英文摘要

Aim: To examine the subcellular distribution of the 3 alpha(1)-adrenoceptor (alpha(1)-AR) subtypes and their internalization and trafficking upon agonist stimulation in human embryonic kidney 293 A cells. Methods: Confocal real-time imaging, enzyme linked immunosorbent assay (ELISA) and whole cell [H-3]-prazosin binding assay were applied to detect the distribution and localization of the 3 alpha(1)-AR subtypes. Results: alpha(1A)-AR was found both on the cell surface and in the cytoplasm; alpha(1B)-AR, however, was predominantly detected on the cell surface, while alpha(1D)-AR was detected mainly in the intracellular compartments. After stimulation with phenylephrine, localization changes were detected by confocal microscopy for alpha(1A)- and alpha(1B)-AR, but the localization of alpha(1D)-AR were unaffected. Phenylephrine stimulation promoted a more rapid internalization of alpha(1B)-AR than alpha(1A)-AR. alpha(1D)-AR internalization was detected only by ELISA. Whole cell [H-3]-prazosin binding assay showed that alpha(1A)-AR functional receptors were detected both on the cell surface and in the cytoplasm; alpha(1B)-AR, however, were detected predominantly on the cell surface, while alpha(1D)-AR were detected mainly in intracellular compartments. Phenylephrine stimulation promoted internalization of alpha(1A)- and alpha(1B)-AR. Conclusion: Phenylephrine stimulation induced changes in the localization of the 3 alpha(1)-AR.

语种英语
WOS记录号WOS:000244418000007
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59325
专题北京大学第三临床医学院_心血管内科
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Ctr Med Anal, Beijing 100083, Peoples R China
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GB/T 7714
Wang, Shu-yi,Song, Yao,Xu, Ming,et al. Internalization and distribution of three alpha(1)-adrenoceptor subtypes in HEK293A cells before and after agonist stimulation[J]. ACTA PHARMACOLOGICA SINICA,2007,28(3):359-366.
APA Wang, Shu-yi,Song, Yao,Xu, Ming,He, Qi-hua,Han, Qi-de,&Zhang, You-yi.(2007).Internalization and distribution of three alpha(1)-adrenoceptor subtypes in HEK293A cells before and after agonist stimulation.ACTA PHARMACOLOGICA SINICA,28(3),359-366.
MLA Wang, Shu-yi,et al."Internalization and distribution of three alpha(1)-adrenoceptor subtypes in HEK293A cells before and after agonist stimulation".ACTA PHARMACOLOGICA SINICA 28.3(2007):359-366.
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