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Identification of Mycobacterium marinum 65 kD heat shock protein gene by polymerase chain reaction restriction analysis from lesions of swimming pool granuloma
Cai, L; Chen, X; Zhao, T; Ding, BC; Zhang, JZ
关键词mycobacterium skin diseases infectious polymerase chain reaction restriction enzyme analysis
刊名CHINESE MEDICAL JOURNAL
2006-01-05
119期:1页:43-48
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]NONTUBERCULOUS MYCOBACTERIA ; LENGTH-POLYMORPHISM ; RAPID IDENTIFICATION ; CUTANEOUS INFECTIONS ; DIAGNOSIS ; HYBRIDIZATION ; EPIDEMIOLOGY ; HAEMOPHILUM ; TESTS ; DNA
英文摘要

Background Nontuberculous mycobacterium (NTM) had been reported to cause cutaneous infections which are difficult to interpret due to the variability of the clinical manifestations. Among NTM infections, Mycobacterium marinum (M. marinum) are mostly seen to cause skin infection. It is therefore important to establish a rapid approach for detection and identification of M. marinum from lesions of patients with suspected M. marinum infections.

Methods Specimens were obtained from 5 patients with swimming pool granuloma. DNA was extracted and polymerase chain reaction (PCR) was performed. PCR products were digested with Hae III and BstE II, then analysed by pattern restriction analysis to detect heat shock protein (hsp) 65 kD gene.

Results The 65 kD hsp gene was found in all specimens from patients with swimming pool granuloma. PCR restriction analysis (PRA) identified all 5 samples to be M. marinum infections, and the result was consistent with that of routine bacteriological identification. The lesions subsided or markedly improved upon treatment.

Conclusions PRA is a sensitive, specific and rapid method in identification of mycobacteria. Application of this method will be helpful for early diagnosis of mycobacterial skin infections.

语种英语
WOS记录号WOS:000234623600008
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59328
专题北京大学第二临床医学院_皮科
作者单位1.Peking Univ, Peoples Hosp, Dept Dermatol, Beijing 100044, Peoples R China
2.Beijing Inst TB Control, Beijing 100035, Peoples R China
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Cai, L,Chen, X,Zhao, T,et al. Identification of Mycobacterium marinum 65 kD heat shock protein gene by polymerase chain reaction restriction analysis from lesions of swimming pool granuloma[J]. CHINESE MEDICAL JOURNAL,2006,119(1):43-48.
APA Cai, L,Chen, X,Zhao, T,Ding, BC,&Zhang, JZ.(2006).Identification of Mycobacterium marinum 65 kD heat shock protein gene by polymerase chain reaction restriction analysis from lesions of swimming pool granuloma.CHINESE MEDICAL JOURNAL,119(1),43-48.
MLA Cai, L,et al."Identification of Mycobacterium marinum 65 kD heat shock protein gene by polymerase chain reaction restriction analysis from lesions of swimming pool granuloma".CHINESE MEDICAL JOURNAL 119.1(2006):43-48.
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