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学科主题: 临床医学
题名:
Determination of cilnidipine, a new calcium antagonist, in human plasma using high performance liquid chromatography with tandem mass spectrometric detection
作者: Zhang, Xianhua; Zhai, Suodi; Zhao, Rongsheny; Jin Ouyang; Li, Xiaoquang; Baeyens, Willy R. G.
关键词: cilnidipine ; nimodipine ; high performance liquid ; chromatographic method coupled ; with tandem mass spectrometry ; (HPLC-MS/MS) ; human plasma
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2007-09-26
DOI: 10.1016/j.aca.2006.11.072
卷: 600, 期:1-2, 页:142-146
收录类别: ISTP ; SCI
文章类型: Proceedings Paper
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: FELODIPINE ; QUANTIFICATION ; LERCANIDIPINE ; LACIDIPINE ; METABOLITE ; ASSAY
英文摘要:

A rapid, sensitive and reliable high performance liquid chromatographic method coupled with tandem mass spectrometry (HPLC-MS/MS) has been developed and validated for the determination of cilnidipine, a relatively new calcium antagonist, in human plasma. The reversed-phase chromatographic system was interfaced with a TurbolonSpray (TIS) source. Nimodipine was employed as the internal standard (IS). Sample extracts following protein precipitation were injected into the HPLC-MS/MS system. The analyte and IS were eluted isocratically on a C18 column, with a mobile phase consisting of CH3OH and NH4Ac (96:4, v/v). The ions were detected by a triple quadrupole mass spectrometric detector in the negative mode. Quantification was performed using multiple reaction monitoring (MRM) of the transitions m/z 491.2 -> 122.1 and m/z 417.1 122.1 for cilnidipine and for the IS, respectively. The analysis time for each run was 3.0 min. The calibration curve fitted well over the concentration range of 0.1-10 ng mL(-1), with the regression equation Y = (0.103 +/- 0.002)X + (0.014 +/- 0.003) (n = 5), r = 0.9994. The intra-day and inter-day R.S.D.% were less than 12.51% at all concentration levels within the calibration range. The recoveries were between 92.71% and 97.64%. The long-term stability and freeze-thaw stability were satisfying at each level. The present method provides a modem, rapid and robust tool for pharmacokinetic studies of cilnidipine. (C) 2006 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000250457500021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59351
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, TDMCt Ctr, Hosp 3, Beijing 100083, Peoples R China
2.Univ Ghent, Fac Pharmaceut Sci, B-9000 Ghent, Belgium
3.Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China

Recommended Citation:
Zhang, Xianhua,Zhai, Suodi,Zhao, Rongsheny,et al. Determination of cilnidipine, a new calcium antagonist, in human plasma using high performance liquid chromatography with tandem mass spectrometric detection[J]. ANALYTICA CHIMICA ACTA,2007,600(1-2):142-146.
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