IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
学科主题临床医学
Molecular identification tools for sibling species of Scedosporium and Pseudallescheria
Lackner, M.1,2,3; Klaassen, C. H.4; Meis, J. F.4; van den Ende, A. H. G. Gerrits1; de Hoog, G. S.1,5,6,7
关键词Internal Transcribed Spacer beta-tubulin RFLP AFLP species-specific primers Pseudallescheria Scedosporium
刊名MEDICAL MYCOLOGY
2012-07-01
DOI10.3109/13693786.2011.618939
50期:5页:497-508
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Mycology ; Veterinary Sciences
研究领域[WOS]Mycology ; Veterinary Sciences
关键词[WOS]BOYDII BRAIN-ABSCESS ; CYSTIC-FIBROSIS ; APIOSPERMUM ; COMPLEX ; GRAPHIUM ; ECOLOGY ; MICROASCACEAE ; PROLIFICANS ; PHYLOGENY ; DIVERSITY
英文摘要

The aim of this study was to develop molecular identification tools for currently recognized species of Pseudallescheria and Scedosporium through the use of species-specific primers and RFLP, so as to enhance rapid differentiation of clinically relevant species. The variability of species was established in a set of 681 Internal Transcribed Spacer (ITS) and 349 beta-tubulin (BT2) sequences. Amplified Fragment Length Polymorphism profile clustering matched with BT2 results, whereas ITS grouping was less detailed. ITS was sufficient for the differentiation of most haplotypes of clinically relevant species (P. apiosperma, P. boydii, S. aurantiacum, S. dehoogii, and S. prolificans) and of environmental species (P. minutispora and Lophotrichus fimeti) when Restriction Fragment Length Polymorphism (RFLP) were applied. For the identification of P. apiosperma and P. boydii species-specific BT2 primers were needed. Pseudallescheria fusoidea, P. ellipsoidea and P. angusta remained difficult to distinguish from P. boydii

语种英语
WOS记录号WOS:000305076200006
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59357
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Sun Yat Sen Univ Hosp, Guangzhou, Guangdong, Peoples R China
2.CBS KNAW Fungal Biodivers Ctr, NL-3508 AD Utrecht, Netherlands
3.Fed Inst Drugs & Med Devices, Bonn, Germany
4.Univ Innsbruck, Inst Microbiol, A-6020 Innsbruck, Austria
5.Canisius Wilhelmina Hosp, Nijmegen, Netherlands
6.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands
7.Peking Univ, Ctr Hlth, Res Ctr Med Mycol, Beijing 100871, Peoples R China
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Lackner, M.,Klaassen, C. H.,Meis, J. F.,et al. Molecular identification tools for sibling species of Scedosporium and Pseudallescheria[J]. MEDICAL MYCOLOGY,2012,50(5):497-508.
APA Lackner, M.,Klaassen, C. H.,Meis, J. F.,van den Ende, A. H. G. Gerrits,&de Hoog, G. S..(2012).Molecular identification tools for sibling species of Scedosporium and Pseudallescheria.MEDICAL MYCOLOGY,50(5),497-508.
MLA Lackner, M.,et al."Molecular identification tools for sibling species of Scedosporium and Pseudallescheria".MEDICAL MYCOLOGY 50.5(2012):497-508.
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