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Auto-mobilized adult hematopoietic stem cells advance neovasculature in diabetic retinopathy of mice
Tian Bei2; Li Xiao-xin1; Shen Li1; Zhao Min1; Yu Wen-zhen1
关键词adult. hematapoietic stem cells diabetic retinopathy stem cells
刊名CHINESE MEDICAL JOURNAL
2010-08-20
DOI10.3760/cma.j.issn.0366-6999.2010.16.020
123期:16页:2265-2268
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]MONOCLONAL-ANTIBODIES ; ANGIOGENESIS ; BLOOD
英文摘要

Background Hematopoietic stem cells (HSCs) can be used to deliver functionally active angiostatic molecules to the retinal vasculature by targeting active astrocytes and may be useful in targeting pre-angiogenic retinal lesions We sought to determine whether HSC mobilization can ameliorate early diabetic retinopathy in mice

Methods Mice were devided into four groups: normal mice control group, normal mice HSC-mobilized group, diabetic mice control group and diabetic mice HSC mobilized group. Murine stem cell growth factor (murine SCF) and recombined human granulocyte colony stimulating factor (rhG-csf) were administered to the mice with diabetes and without diabetes for continuous 5 days to induce autologous HSCs mobilization, and subcutaneous injection of physiological saline was used as control. Immunohistochemical double staining was conducted with anti-mouse rat CD31 monoclonal antibody and anti-BrdU rat antibody

Results Marked HSCs clearly increased after SCF plus G-csf-mobilization Non-mobilized diabetic mice showed more HSCs than normal mice (P=0.032), and peripheral blood significantly increased in both diabetic and normal mice (P=0.000) Diabetic mice showed more CD31 positive capillary vessels (P=0 000) and accelerated endothelial cell regeneration Only diabetic HSC-mobilized mice expressed both BrdU and CD31 antigens in the endothelial cells of new capillaries.

Conclusion Auto-mobilized adult hematopoietic stem cells advance neovasculature in diabetic retinopathy of mice

语种英语
WOS记录号WOS:000281488300020
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59370
专题北京大学第二临床医学院_眼科
作者单位1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Capital Med Univ, Beijing Ophthalmol & Visual Sci Key Lab, Beijing Tongren Eye Ctr, Beijing 100730, Peoples R China
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Tian Bei,Li Xiao-xin,Shen Li,et al. Auto-mobilized adult hematopoietic stem cells advance neovasculature in diabetic retinopathy of mice[J]. CHINESE MEDICAL JOURNAL,2010,123(16):2265-2268.
APA Tian Bei,Li Xiao-xin,Shen Li,Zhao Min,&Yu Wen-zhen.(2010).Auto-mobilized adult hematopoietic stem cells advance neovasculature in diabetic retinopathy of mice.CHINESE MEDICAL JOURNAL,123(16),2265-2268.
MLA Tian Bei,et al."Auto-mobilized adult hematopoietic stem cells advance neovasculature in diabetic retinopathy of mice".CHINESE MEDICAL JOURNAL 123.16(2010):2265-2268.
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