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学科主题: 临床医学
题名:
Auto-mobilized adult hematopoietic stem cells advance neovasculature in diabetic retinopathy of mice
作者: Tian Bei2; Li Xiao-xin1; Shen Li1; Zhao Min1; Yu Wen-zhen1
关键词: adult. hematapoietic stem cells ; diabetic retinopathy ; stem cells
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2010-08-20
DOI: 10.3760/cma.j.issn.0366-6999.2010.16.020
卷: 123, 期:16, 页:2265-2268
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: MONOCLONAL-ANTIBODIES ; ANGIOGENESIS ; BLOOD
英文摘要:

Background Hematopoietic stem cells (HSCs) can be used to deliver functionally active angiostatic molecules to the retinal vasculature by targeting active astrocytes and may be useful in targeting pre-angiogenic retinal lesions We sought to determine whether HSC mobilization can ameliorate early diabetic retinopathy in mice

Methods Mice were devided into four groups: normal mice control group, normal mice HSC-mobilized group, diabetic mice control group and diabetic mice HSC mobilized group. Murine stem cell growth factor (murine SCF) and recombined human granulocyte colony stimulating factor (rhG-csf) were administered to the mice with diabetes and without diabetes for continuous 5 days to induce autologous HSCs mobilization, and subcutaneous injection of physiological saline was used as control. Immunohistochemical double staining was conducted with anti-mouse rat CD31 monoclonal antibody and anti-BrdU rat antibody

Results Marked HSCs clearly increased after SCF plus G-csf-mobilization Non-mobilized diabetic mice showed more HSCs than normal mice (P=0.032), and peripheral blood significantly increased in both diabetic and normal mice (P=0.000) Diabetic mice showed more CD31 positive capillary vessels (P=0 000) and accelerated endothelial cell regeneration Only diabetic HSC-mobilized mice expressed both BrdU and CD31 antigens in the endothelial cells of new capillaries.

Conclusion Auto-mobilized adult hematopoietic stem cells advance neovasculature in diabetic retinopathy of mice

语种: 英语
WOS记录号: WOS:000281488300020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59370
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Capital Med Univ, Beijing Ophthalmol & Visual Sci Key Lab, Beijing Tongren Eye Ctr, Beijing 100730, Peoples R China

Recommended Citation:
Tian Bei,Li Xiao-xin,Shen Li,et al. Auto-mobilized adult hematopoietic stem cells advance neovasculature in diabetic retinopathy of mice[J]. CHINESE MEDICAL JOURNAL,2010,123(16):2265-2268.
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