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学科主题: 临床医学
题名:
Effects of combined epidermal growth factor, brain-derived neurotrophic factor and insulin-like growth factor-1 on human oocyte maturation and early fertilized and cloned embryo development
作者: Yu, Yang1,2,3; Yan, Jie1,3; Li, Min1,3; Yan, Liying1,3; Zhao, Yu1,3; Lian, Ying1,3; Li, Rong1; Liu, Ping1,3; Qiao, Jie1,2,3
关键词: growth factors ; in vitro maturation ; human oocytes ; embryo development ; somatic cell nuclear transfer
刊名: HUMAN REPRODUCTION
发表日期: 2012-07-01
DOI: 10.1093/humrep/des099
卷: 27, 期:7, 页:2146-2159
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: CELL NUCLEAR TRANSFER ; PREIMPLANTATION EMBRYO ; VITRO MATURATION ; CYTOPLASMIC MATURATION ; PORCINE OOCYTES ; IGF-I ; COMPETENCE ; CULTURE ; QUALITY ; DERIVATION
英文摘要:

Human cloned blastocysts generated from oocytes following in vitro maturation (IVM) are a potential resource for embryonic stem cells (ESC) with homologous immune systems. The purpose of this study was to evaluate the effects of multiple growth factors [epidermal growth factor (EGF), brain-derived neurotrophic factor (BDNF) and insulin-like growth factor-1 (IGF-1)] on human oocyte maturation, early embryo development, blastocyst formation and ESC line generation.

Patients (n 344) undergoing IVF owing to male factor infertility were enrolled in this study. Metaphase II oocytes were separated into four grades based on their morphology. Spindle assembly from IVM oocytes with or without growth factor treatment was assessed by immunostaining. Piezo-assisted micromanipulation technology was used to produce fertilized (ICSI) and cloned [(somatic cell nuclear transfer (SCNT)] embryos. Embryos received four different growth factor treatments; embryo development rates from pronuclear to blastocyst stage and embryo grading (for quality) at the 8-cell stage were analyzed. The presence of receptors on human cumulus cells and IVM oocytes was assessed by immunofluorescence. The blastocysts generated from fertilized and cloned embryos were used for ESC derivation.

The combination of EGF, BDNF and IGF-1 can effectively increase oocyte maturation rate in vitro, and significantly improve the oocyte quality in terms of morphology and normal spindle levels (P 0.05). Also, the developmental competence of fertilized oocytes to 8-cell and blastocyst stages was improved by the addition of growth factors (P 0.05). However, there were no significant differences among the four groups in 8-cell grading. Blastocyst formation in cloned embryos cultured with the three growth factors was higher than the control group (23.1 versus 4.3, P 0.05). Receptors for the three growth factors were present in cumulus cells and IVM oocytes, and four human ESC lines were derived from fertilized blastocysts but none from cloned blastocysts.

This study demonstrated that EGF, BDNF and IGF-1 can improve oocyte maturation rate and quality in vitro, and consequently increase early embryo development and blastocyst formation, which is very beneficial in improving the reprogramming efficiency of SCNT. The present study has identified a valuable culture system for IVM and cloned human embryos, potentially using these embryos to derive human therapeutic ESC.

语种: 英语
所属项目编号: 2011CB944504 ; 31000661
项目资助者: Ministry of Science and Technology of China Grants (973 program) ; National Natural Science Funds for Distinguished Young Scholar
WOS记录号: WOS:000305458800029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59373
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
2.Peking Univ, Dept Obstet & Gynecol, Ctr Reprod Med, Hosp 3, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China

Recommended Citation:
Yu, Yang,Yan, Jie,Li, Min,et al. Effects of combined epidermal growth factor, brain-derived neurotrophic factor and insulin-like growth factor-1 on human oocyte maturation and early fertilized and cloned embryo development[J]. HUMAN REPRODUCTION,2012,27(7):2146-2159.
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