学科主题临床医学
Dynamic Metabonomic Analysis of BALB/c Mice with Different Outcomes After D-Galactosamine/Lipopolysaccharide-Induced Fulminant Hepatic Failure
Feng, Bo2; Wu, Shengming1; Lv, Sa2; Fang, Junjian1; Liu, Feng2; Li, Yu1; Gao, Yan2; Yan, Xianzhong1; Dong, Fangting1; Wei, Lai2
刊名LIVER TRANSPLANTATION
2008-11-01
DOI10.1002/lt.21578
14期:11页:1620-1631
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology ; Surgery ; Transplantation
研究领域[WOS]Gastroenterology & Hepatology ; Surgery ; Transplantation
关键词[WOS]ACUTE LIVER-FAILURE ; UNITED-STATES MULTICENTER ; D-GALACTOSAMINE ; RAT-LIVER ; H-1-NMR-BASED METABONOMICS ; AMINO-ACID ; MODEL ; METABOLISM ; CRITERIA ; LACTATE
英文摘要

Fulminant hepatic failure (FHF) is one of the most challenging gastrointestinal emergencies encountered in clinical practice. Early identification of patients with FHF who need liver transplantation is very important. To construct a prediction model for the early diagnosis and prognosis of FHF, we studied the dynamics of metabolic intermediates and metabolic profiles with a D-galactosamine (GaIN)/lipopolysaccharide (LPS)-treated BALB/c mouse model of FHF. Levels of plasma metabolites were quantified with gas chromatography/time-of-flight mass spectrometry, and data were processed with partial least squares discriminant analysis (PLS-DA). Distinct clustering differences were observed 5 and 6 hours after GaIN/LPS treatment between mice that survived and those that died, but there were no differences between these groups 4 hours after treatment. Five hours after treatment, plasma levels of some metabolites differed significantly between the survival, dead, and control groups. Ketogenesis and the tricarboxylic acid cycle were inhibited in both the survival and dead groups, but in the dead group, the urea cycle was also inhibited, and glycolysis was elevated. PLS-DA indicated that principal component weighting was greatest for plasma levels of phosphate, beta-hydroxybutyrate, urea, glucose, and lactate. The Y-predicted scatter plot in the partial least squares (PLS) model assigned samples to the survival or dead groups with an a priori cutoff of 0.10 with 100% sensitivity and specificity. Similar results were observed in 11 FHF patients with different outcomes. In conclusion, the PLS model based on metabonomic analysis can be used to predict outcomes well, and plasma levels of phosphate, beta-hydroxybutyrate, urea, glucose, and lactate may constitute a set of markers for the early diagnosis and prognosis of FHF. Liver Transpl 14: 1620-1631, 2008. (C) 2008 AASLD.

语种英语
WOS记录号WOS:000260894900014
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59378
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位1.Natl Ctr Biomed Anal, Beijing 100039, Peoples R China
2.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Feng, Bo,Wu, Shengming,Lv, Sa,et al. Dynamic Metabonomic Analysis of BALB/c Mice with Different Outcomes After D-Galactosamine/Lipopolysaccharide-Induced Fulminant Hepatic Failure[J]. LIVER TRANSPLANTATION,2008,14(11):1620-1631.
APA Feng, Bo.,Wu, Shengming.,Lv, Sa.,Fang, Junjian.,Liu, Feng.,...&Wei, Lai.(2008).Dynamic Metabonomic Analysis of BALB/c Mice with Different Outcomes After D-Galactosamine/Lipopolysaccharide-Induced Fulminant Hepatic Failure.LIVER TRANSPLANTATION,14(11),1620-1631.
MLA Feng, Bo,et al."Dynamic Metabonomic Analysis of BALB/c Mice with Different Outcomes After D-Galactosamine/Lipopolysaccharide-Induced Fulminant Hepatic Failure".LIVER TRANSPLANTATION 14.11(2008):1620-1631.
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