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学科主题临床医学
Berberine ameliorates renal interstitial fibrosis induced by unilateral ureteral obstruction in rats
Wang, Fang-Ming1; Yang, Yu-Jie2; Ma, Lu-Lin1; Tian, Xiao-Jun1; He, Ya-Qiang1
关键词berberine fibrosis oxidative stress rats ureteral obstruction
刊名NEPHROLOGY
2014-09-01
DOI10.1111/nep.12271
19期:9页:542-551
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]GROWTH-FACTOR-BETA ; OXIDATIVE STRESS ; KIDNEY ; INJURY ; CELLS ; NEPHROPATHY ; ACTIVATION ; EXPRESSION ; APOPTOSIS ; INSIGHTS
英文摘要

Aim: To investigate the potential effects of berberine on renal interstitial fibrosis (RIF) of obstructed kidneys in a unilateral ureteral obstruction (UUO) rat model.

Methods: Forty-eight rats were randomly divided into three groups: shamoperated, vehicle-treated UUO, and berberine-treated UUO. Rats were gavaged with berberine (200 mg/kg per day) or vehicle. Eight randomly chosen rats in each group were kiled and specimens were collected at day 14 after UUO. Physiological parameters and histological changes were assessed, RIF was evaluated using Masson′s trichrome and Sirius red staining, oxidative stress and inflammation markers were determined, transforming growth factor beta 1 (TGF-beta 1), phosphorylated Smad3 (pSmad3) and alpha-smooth muscle actin (alpha-SMA) were measured using immunohistochemistry or western blotting analysis. The obstruction was relieved at day 14 by percutaneous nephrostomy in the remaining UUO rats. The resistive index of left kidneys was undertaken by coloured Doppler flow imaging at day 14 before nephrostomy and day 7 after the relief.

Results: Berberine treatment significantly attenuated RIF induced by UUO. The UUO-induced reduction in kidney superoxide dismutase and catalase activities increased, whereas elevated kidney malondialdehyde level markedly decreased. Berberine treatment significantly ameliorated UUO-induced inflammation, and decreased TGF-beta 1, pSmad3 and alpha-SMA expression of UUO kidneys. Moreover, berberine treatment significantly suppressed the increase of resistive index compared with UUO group at day 14 after UUO as well as day 7 after the relief of obstruction.

Conclusion: Berberine treatment ameliorates RIF in a UUO rat model by inhibition of oxidative stress, inflammatory responses, and TGF-beta 1/pSmad3 signalling.

语种英语
WOS记录号WOS:000341136300004
项目编号30950018 ; 7113174
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59383
专题北京大学第三临床医学院_泌尿外科
作者单位1.Peking Univ, Hosp 3, Dept Urol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Diagnost Ultrasound, Beijing 100191, Peoples R China
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GB/T 7714
Wang, Fang-Ming,Yang, Yu-Jie,Ma, Lu-Lin,et al. Berberine ameliorates renal interstitial fibrosis induced by unilateral ureteral obstruction in rats[J]. NEPHROLOGY,2014,19(9):542-551.
APA Wang, Fang-Ming,Yang, Yu-Jie,Ma, Lu-Lin,Tian, Xiao-Jun,&He, Ya-Qiang.(2014).Berberine ameliorates renal interstitial fibrosis induced by unilateral ureteral obstruction in rats.NEPHROLOGY,19(9),542-551.
MLA Wang, Fang-Ming,et al."Berberine ameliorates renal interstitial fibrosis induced by unilateral ureteral obstruction in rats".NEPHROLOGY 19.9(2014):542-551.
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