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学科主题: 临床医学
题名:
Berberine ameliorates renal interstitial fibrosis induced by unilateral ureteral obstruction in rats
作者: Wang, Fang-Ming1; Yang, Yu-Jie2; Ma, Lu-Lin1; Tian, Xiao-Jun1; He, Ya-Qiang1
关键词: berberine ; fibrosis ; oxidative stress ; rats ; ureteral obstruction
刊名: NEPHROLOGY
发表日期: 2014-09-01
DOI: 10.1111/nep.12271
卷: 19, 期:9, 页:542-551
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: GROWTH-FACTOR-BETA ; OXIDATIVE STRESS ; KIDNEY ; INJURY ; CELLS ; NEPHROPATHY ; ACTIVATION ; EXPRESSION ; APOPTOSIS ; INSIGHTS
英文摘要:

Aim: To investigate the potential effects of berberine on renal interstitial fibrosis (RIF) of obstructed kidneys in a unilateral ureteral obstruction (UUO) rat model.

Methods: Forty-eight rats were randomly divided into three groups: shamoperated, vehicle-treated UUO, and berberine-treated UUO. Rats were gavaged with berberine (200 mg/kg per day) or vehicle. Eight randomly chosen rats in each group were kiled and specimens were collected at day 14 after UUO. Physiological parameters and histological changes were assessed, RIF was evaluated using Masson′s trichrome and Sirius red staining, oxidative stress and inflammation markers were determined, transforming growth factor beta 1 (TGF-beta 1), phosphorylated Smad3 (pSmad3) and alpha-smooth muscle actin (alpha-SMA) were measured using immunohistochemistry or western blotting analysis. The obstruction was relieved at day 14 by percutaneous nephrostomy in the remaining UUO rats. The resistive index of left kidneys was undertaken by coloured Doppler flow imaging at day 14 before nephrostomy and day 7 after the relief.

Results: Berberine treatment significantly attenuated RIF induced by UUO. The UUO-induced reduction in kidney superoxide dismutase and catalase activities increased, whereas elevated kidney malondialdehyde level markedly decreased. Berberine treatment significantly ameliorated UUO-induced inflammation, and decreased TGF-beta 1, pSmad3 and alpha-SMA expression of UUO kidneys. Moreover, berberine treatment significantly suppressed the increase of resistive index compared with UUO group at day 14 after UUO as well as day 7 after the relief of obstruction.

Conclusion: Berberine treatment ameliorates RIF in a UUO rat model by inhibition of oxidative stress, inflammatory responses, and TGF-beta 1/pSmad3 signalling.

语种: 英语
所属项目编号: 30950018 ; 7113174
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000341136300004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59383
Appears in Collections:北京大学第三临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Urol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Diagnost Ultrasound, Beijing 100191, Peoples R China

Recommended Citation:
Wang, Fang-Ming,Yang, Yu-Jie,Ma, Lu-Lin,et al. Berberine ameliorates renal interstitial fibrosis induced by unilateral ureteral obstruction in rats[J]. NEPHROLOGY,2014,19(9):542-551.
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