IR@PKUHSC  > 北京大学第二临床医学院  > 眼科
学科主题临床医学
Quercetin inhibits choroidal and retinal angiogenesis in vitro
Chen, Yi1; Li, Xiao-xin1; Xing, Nian-zeng2; Cao, Xiao-guang1
关键词quercetin angiogenesis apoptosis
刊名GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
2008-03-01
DOI10.1007/s00417-007-0728-9
246期:3页:373-378
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]DIABETIC-RETINOPATHY ; ENDOTHELIAL-CELLS ; ANTIOXIDANT CAPACITY ; CANCER CELLS ; FLAVONOIDS ; KINASE ; GROWTH ; PROGRESSION ; MECHANISMS ; ACID
英文摘要

Background Quercetin is a natural substance found abundantly in grapes, red wine and other food products. In this study, we examined the effect of quercetin on choroidal and retinal angiogenesis in vitro using rhesus choroids-retina endothelial cell line (RF/6A).

Methods RF/6A cells were cultured in Dulbecco′s Modified Eagle′s Medium (DMEM) containing 10% fetal bovine serum. Then cells were treated with different concentrations (from 0 to 100 mu M) of quercetin. The cell proliferation was assessed using choromogenic methylthiazol tetrazolium bromide (MTT) dye after 24, 48 and 72 hours. Cell migration after 24-hour incubation with quercetin was investigated by wound assay. Following exposure to the various concentrations of quercetin for 24 hours, tube formation on matrigel by endothelial cells was also analyzed. Apoptosis was measured by flow cytometry using annexin V-FITC and propidium iodide staining.

Results Quercetin inhibits endothelial cell proliferation in a dose-dependent fashion; 10.1%, 42.6% and 65.2% inhibition on treating with 10, 50 and 100 mu M Quercetin respectively. The migration and tube formation of RA/6A cells were also significantly inhibited by quercetin in a dose-dependent manner. Flow cytometric analysis showed that the percentages of apoptotic cells were slightly increased only in 100 mu M quercetin-treated cells.

Conclusions Our results show that quercetin inhibits choroidal and retinal angiogenesis in vitro. Further studies are ongoing to evaluate this drug as a potential candidate for the treatment of choroidal or retinal neovascularization.

语种英语
WOS记录号WOS:000252992000008
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59401
专题北京大学第二临床医学院_眼科
作者单位1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Capital Univ Med Sci, Dept Urol, Beijing Chaoyang Hosp, Beijing, Peoples R China
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GB/T 7714
Chen, Yi,Li, Xiao-xin,Xing, Nian-zeng,et al. Quercetin inhibits choroidal and retinal angiogenesis in vitro[J]. GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY,2008,246(3):373-378.
APA Chen, Yi,Li, Xiao-xin,Xing, Nian-zeng,&Cao, Xiao-guang.(2008).Quercetin inhibits choroidal and retinal angiogenesis in vitro.GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY,246(3),373-378.
MLA Chen, Yi,et al."Quercetin inhibits choroidal and retinal angiogenesis in vitro".GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY 246.3(2008):373-378.
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