北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 眼科  > 期刊论文
学科主题: 临床医学
题名:
Quercetin inhibits choroidal and retinal angiogenesis in vitro
作者: Chen, Yi1; Li, Xiao-xin1; Xing, Nian-zeng2; Cao, Xiao-guang1
关键词: quercetin ; angiogenesis ; apoptosis
刊名: GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
发表日期: 2008-03-01
DOI: 10.1007/s00417-007-0728-9
卷: 246, 期:3, 页:373-378
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: DIABETIC-RETINOPATHY ; ENDOTHELIAL-CELLS ; ANTIOXIDANT CAPACITY ; CANCER CELLS ; FLAVONOIDS ; KINASE ; GROWTH ; PROGRESSION ; MECHANISMS ; ACID
英文摘要:

Background Quercetin is a natural substance found abundantly in grapes, red wine and other food products. In this study, we examined the effect of quercetin on choroidal and retinal angiogenesis in vitro using rhesus choroids-retina endothelial cell line (RF/6A).

Methods RF/6A cells were cultured in Dulbecco′s Modified Eagle′s Medium (DMEM) containing 10% fetal bovine serum. Then cells were treated with different concentrations (from 0 to 100 mu M) of quercetin. The cell proliferation was assessed using choromogenic methylthiazol tetrazolium bromide (MTT) dye after 24, 48 and 72 hours. Cell migration after 24-hour incubation with quercetin was investigated by wound assay. Following exposure to the various concentrations of quercetin for 24 hours, tube formation on matrigel by endothelial cells was also analyzed. Apoptosis was measured by flow cytometry using annexin V-FITC and propidium iodide staining.

Results Quercetin inhibits endothelial cell proliferation in a dose-dependent fashion; 10.1%, 42.6% and 65.2% inhibition on treating with 10, 50 and 100 mu M Quercetin respectively. The migration and tube formation of RA/6A cells were also significantly inhibited by quercetin in a dose-dependent manner. Flow cytometric analysis showed that the percentages of apoptotic cells were slightly increased only in 100 mu M quercetin-treated cells.

Conclusions Our results show that quercetin inhibits choroidal and retinal angiogenesis in vitro. Further studies are ongoing to evaluate this drug as a potential candidate for the treatment of choroidal or retinal neovascularization.

语种: 英语
WOS记录号: WOS:000252992000008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59401
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Capital Univ Med Sci, Dept Urol, Beijing Chaoyang Hosp, Beijing, Peoples R China

Recommended Citation:
Chen, Yi,Li, Xiao-xin,Xing, Nian-zeng,et al. Quercetin inhibits choroidal and retinal angiogenesis in vitro[J]. GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY,2008,246(3):373-378.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen, Yi]'s Articles
[Li, Xiao-xin]'s Articles
[Xing, Nian-zeng]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen, Yi]‘s Articles
[Li, Xiao-xin]‘s Articles
[Xing, Nian-zeng]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace