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学科主题: 基础医学
题名:
Tonsillar Crypt Epithelium Is an Important Extra-Central Nervous System Site for Viral Replication in EV71 Encephalomyelitis
作者: He, Yaoxin1; Ong, Kien Chai2; Gao, Zifen1; Zhao, Xishun1; Anderson, Virginia M.4; McNutt, Michael. A.4; Kum Thong Wong3; Lu, Min1
刊名: AMERICAN JOURNAL OF PATHOLOGY
发表日期: 2014-03-01
DOI: 10.1016/j.ajpath.2013.11.009
卷: 184, 期:3, 页:714-720
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pathology
研究领域[WOS]: Pathology
关键词[WOS]: ENTEROVIRUS 71 INFECTION ; SELECTIN GLYCOPROTEIN LIGAND-1 ; MOUTH-DISEASE ; CYNOMOLGUS MONKEYS ; DIAGNOSIS ; TAIWAN ; VIRUS ; ENCEPHALITIS ; INFLAMMATION ; OUTBREAK
英文摘要:

Enterovirus 71 (EV71; family Picornaviridae, species human Enterovirus A) usually causes hand, foot, and mouth disease, which may rarely be complicated by fatal encephalomyelitis. We investigated extra-central nervous system (extra-CNS) tissues capable of supporting EV71 infection and replication, and have correlated tissue infection with expression of putative viral entry receptors, scavenger receptor B2 (SCARB2), and P-selectin glycoprotein Ligand-1 (PSGL-1). Formalin-fixed, paraffin-embedded CNS and extra-CNS tissues from seven autopsy cases were examined by IHC and in situ hybridization to evaluate viral antigens and RNA. Viral receptors were identified with IHC. In all seven cases, the CNS showed stereotypical distribution of inflammation and neuronal localization of viral antigens and RNA, confirming the clinical diagnosis of EV71 encephalomyelitis. In six cases in which tonsillar tissues were available, viral antigens and/or RNA were localized to squamous epithelium Lining the tonsillar crypts. Tissues from the gastrointestinal tract, pancreas, mesenteric nodes, spleen, and skin were all negative for viral antigens/RNA. Our novel findings strongly suggest that tonsillar crypt squamous epithelium supports active viral replication and represents an important source of viral shedding that facilitates person-to-person transmission by both the fecal-oral or oral-oral routes. It may also be a portal for viral entry. A correlation between viral infection and SCARB2 expression appears to be more significant than for PSGL-1 expression.

语种: 英语
所属项目编号: H-20001-00-E000004 ; FP021/2009 ; RG141/09HTM
项目资助者: University of Malaya/Ministry of Higher Education High Impact Research grant ; University of Malaya ; Ministry of Higher Education (Malaysia) ; University of Malaya (Malaysia)
WOS记录号: WOS:000332055500013
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59406
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Pathol, Beijing 100191, Peoples R China
2.Univ Malaya, Fac Med, Dept Biomed Sci, Kuala Lumpur, Malaysia
3.Univ Malaya, Fac Med, Dept Pathology,t, Kuala Lumpur, Malaysia
4.SUNY Hlth Sci Ctr, Dept Pathol, Brooklyn, NY 11203 USA

Recommended Citation:
He, Yaoxin,Ong, Kien Chai,Gao, Zifen,et al. Tonsillar Crypt Epithelium Is an Important Extra-Central Nervous System Site for Viral Replication in EV71 Encephalomyelitis[J]. AMERICAN JOURNAL OF PATHOLOGY,2014,184(3):714-720.
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