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学科主题: 临床医学
题名:
Identification and characteristics of a novel testis-specific gene, Tsc21, in mice and human
作者: Yu, Zhendong; Tang, Aifa; Gui, Yaoting; Guo, Xin; Zhu, Hui; Long, Yun; Li, Zeting; Cai, Zhiming
关键词: testis-specific gene ; Affymetrix chip ; spermatogenesis
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2007-06-01
DOI: 10.1007/s11033-006-9026-6
卷: 34, 期:2, 页:127-134
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: MOUSE SPERMATOGENESIS ; TRANSGENIC MICE ; GERM-CELLS ; EXPRESSION ; PROTEIN ; MEIOSIS ; ARRAYS ; RNA
英文摘要:

Testis-specific genes are essential for spermatogenesis in mammalian male reproduction. We have identified a novel gene, Tsc21, exclusively expressed in mice and human testes from the results of the Affymetrix Genechip analysis in the six developmental stages of testis of postnatal Balb/C mice. The full cDNA length of Tsc21 was 810 bp, with a 543 bp open reading frame encoding a 180 amino acids protein with a predicted molecular weight of 21.040 kDa. A Blast search in the mouse genome database localized the Tsc21 gene to mice chromosome 6C3. Multiple amino acid sequence alignment of human, mouse, and rat homologous genes showed that mice Tsc21 protein was highly homologous with the human Tsc21 gene (70%) and rat Tsc21 gene (86%). The results of reverse transcriptase-polymerase chain reaction analysis showed that the mice Tsc21 is exclusively expressed in the testis and epididymis of mice, and its expression is only detected after the mice is 35 days old. Human Tsc21 is also exclusively expressed in testis of human. Considering the expression profile Tsc21 in mice and human, we propose that Tsc21 may play a role during mammalian male spermatogenesis. Our study should be a basis for function characterization of the Tsc21 gene, leading to the elucidation of the molecular events underlying mammalian male reproduction.

语种: 英语
WOS记录号: WOS:000246112000007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59418
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: Peking Univ, Shenzhen PKU HKUST Med Ctr, Shenzhen Hosp, Lab Male Reprod Med, Shenzhen 518036, Peoples R China

Recommended Citation:
Yu, Zhendong,Tang, Aifa,Gui, Yaoting,et al. Identification and characteristics of a novel testis-specific gene, Tsc21, in mice and human[J]. MOLECULAR BIOLOGY REPORTS,2007,34(2):127-134.
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