IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Identification and characteristics of a novel testis-specific gene, Tsc21, in mice and human
Yu, Zhendong; Tang, Aifa; Gui, Yaoting; Guo, Xin; Zhu, Hui; Long, Yun; Li, Zeting; Cai, Zhiming
关键词testis-specific gene Affymetrix chip spermatogenesis
刊名MOLECULAR BIOLOGY REPORTS
2007-06-01
DOI10.1007/s11033-006-9026-6
34期:2页:127-134
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]MOUSE SPERMATOGENESIS ; TRANSGENIC MICE ; GERM-CELLS ; EXPRESSION ; PROTEIN ; MEIOSIS ; ARRAYS ; RNA
英文摘要

Testis-specific genes are essential for spermatogenesis in mammalian male reproduction. We have identified a novel gene, Tsc21, exclusively expressed in mice and human testes from the results of the Affymetrix Genechip analysis in the six developmental stages of testis of postnatal Balb/C mice. The full cDNA length of Tsc21 was 810 bp, with a 543 bp open reading frame encoding a 180 amino acids protein with a predicted molecular weight of 21.040 kDa. A Blast search in the mouse genome database localized the Tsc21 gene to mice chromosome 6C3. Multiple amino acid sequence alignment of human, mouse, and rat homologous genes showed that mice Tsc21 protein was highly homologous with the human Tsc21 gene (70%) and rat Tsc21 gene (86%). The results of reverse transcriptase-polymerase chain reaction analysis showed that the mice Tsc21 is exclusively expressed in the testis and epididymis of mice, and its expression is only detected after the mice is 35 days old. Human Tsc21 is also exclusively expressed in testis of human. Considering the expression profile Tsc21 in mice and human, we propose that Tsc21 may play a role during mammalian male spermatogenesis. Our study should be a basis for function characterization of the Tsc21 gene, leading to the elucidation of the molecular events underlying mammalian male reproduction.

语种英语
WOS记录号WOS:000246112000007
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59418
专题北京大学深圳医院
作者单位Peking Univ, Shenzhen PKU HKUST Med Ctr, Shenzhen Hosp, Lab Male Reprod Med, Shenzhen 518036, Peoples R China
推荐引用方式
GB/T 7714
Yu, Zhendong,Tang, Aifa,Gui, Yaoting,et al. Identification and characteristics of a novel testis-specific gene, Tsc21, in mice and human[J]. MOLECULAR BIOLOGY REPORTS,2007,34(2):127-134.
APA Yu, Zhendong.,Tang, Aifa.,Gui, Yaoting.,Guo, Xin.,Zhu, Hui.,...&Cai, Zhiming.(2007).Identification and characteristics of a novel testis-specific gene, Tsc21, in mice and human.MOLECULAR BIOLOGY REPORTS,34(2),127-134.
MLA Yu, Zhendong,et al."Identification and characteristics of a novel testis-specific gene, Tsc21, in mice and human".MOLECULAR BIOLOGY REPORTS 34.2(2007):127-134.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yu, Zhendong]的文章
[Tang, Aifa]的文章
[Gui, Yaoting]的文章
百度学术
百度学术中相似的文章
[Yu, Zhendong]的文章
[Tang, Aifa]的文章
[Gui, Yaoting]的文章
必应学术
必应学术中相似的文章
[Yu, Zhendong]的文章
[Tang, Aifa]的文章
[Gui, Yaoting]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。