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学科主题: 口腔医学
题名:
S100A9-induced release of interleukin (IL)-6 and IL-8 through toll-like receptor 4 (TLR4) in human periodontal ligament cells
作者: Gao, Hongyu1; Zhang, Xin1,2; Zheng, Yunfei1,3; Peng, Lei1,4; Hou, Jianxia1; Meng, Huanxin1
关键词: S100A9 ; Pro-inflammatory effects ; PDL cells
刊名: MOLECULAR IMMUNOLOGY
发表日期: 2015-10-01
DOI: 10.1016/j.molimm.2015.05.014
卷: 67, 期:2, 页:223-232
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Immunology
研究领域[WOS]: Biochemistry & Molecular Biology ; Immunology
关键词[WOS]: GINGIVAL CREVICULAR FLUID ; GLYCATION END-PRODUCTS ; S100A9 ; PROTEINS ; DISEASE ; MRP14 ; EXPRESSION ; INDUCTION ; LIPOPOLYSACCHARIDE ; CALPROTECTIN
英文摘要:

S100A8, S100A9, and calprotectin (the S100A8/S100A9 complex) are calcium-binding proteins that promote extracellular pro-inflammatory functions and may play an important role in periodontal disease. Both toll-like receptor 4 (TLR4) and the receptor for advanced glycation end-products (RAGE) are thought to be important receptors for S1 00A8, Si 00A9, and calprotectin, but the specific pathways in periodontal ligament (PDL) cells are not yet clear. Our study was designed to identify the specific receptors for Si 00A9 in human PDL cells. Additionally, we investigated the specific pathways that activate the secretion of pro-inflammatory cytokines interleukins (IL)-6 and IL-8 in PDL cells. The role of nuclear factor (NF)-kappa B, mitogen-activated protein kinase (MAPK) and reactive oxygen species (ROS) in S100A9-induced proinflammatory cytokines were investigated through western blot analysis, dichlorodihydrofluorescein diacetate (H(2)DCFDA) probe and the application of specific pathway inhibitors. Our results suggest that the S100A9-induced release of IL-6 and IL-8 from human PDL cells is dependent on TLR4, but not RAGE. We provide evidence that S100A9 promotes the secretion of IL-6 and IL-8 through different pathways. Specifically, Si100A9 up-regulates the secretion of IL-6 from human PDL cells through NF-kappa B and p38 pathways and up-regulates the release of IL-8 from human PDL cells through the NF-kappa B, extracellular-regulated kinase (ERK) 1/2, c-Jun amino-terminal kinase UNK) 1/2, and p38 signaling pathways. In addition, the release of both cytokines depends on ROS production. The release of both cytokines depends on ROS production. These results suggest that S100A9 promotes pro-inflammatory responses in PDL cells through the TLR4-mediated NF-kappa B and MAPK signaling pathways. (C) 2015 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 30271411 ; 30471882 ; 30973319 ; 81271149
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000361922400005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59419
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Peking Univ, Dept Periodontol, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
2.Chinese Acad Med Sci, Dept Stomatol, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China
3.Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
4.Capital Med Univ, Beijing Friendship Hosp, Dept Stomatol, Beijing 100050, Peoples R China

Recommended Citation:
Gao, Hongyu,Zhang, Xin,Zheng, Yunfei,et al. S100A9-induced release of interleukin (IL)-6 and IL-8 through toll-like receptor 4 (TLR4) in human periodontal ligament cells[J]. MOLECULAR IMMUNOLOGY,2015,67(2):223-232.
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