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学科主题: 口腔医学
题名:
EphB-EphrinB Interaction Controls Odontogenic/Osteogenic Differentiation with Calcium Hydroxide
作者: Wang, Xiaozhe1,3; Jong, George2; Lin, Louis M.2; Shimizu, Emi1,2
关键词: Calcium hydroxide ; Eph ; ephrin ; primary pulp cells ; pulp capping
刊名: JOURNAL OF ENDODONTICS
发表日期: 2013-10-01
DOI: 10.1016/j.joen.2013.06.016
卷: 39, 期:10, 页:1256-1260
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: DENTIN FORMATION ; ASCORBIC-ACID ; CELLS ; PULP ; TOOTH ; RECEPTORS ; REPAIR
英文摘要:

Introduction: Calcium hydroxide is used in direct pulp capping of uncontaminated exposed vital pulps caused by mechanical or traumatic injury. Calcium hydroxide creates a high alkaline pH environment and initiates a mineralized tissue formation in the pulp. The exact mechanism by which calcium hydroxide induces the reparative dentin formation is unknown. Because Eph receptors and ephrin ligands play a role in pulp stem cell migration and proliferation, our hypothesis is that calcium hydroxide-related odontogenic/osteogenic differentiation may be associated with Eph-ephrin interaction. The aim of this study was to investigate whether Eph-ephrin interaction regulates odontogenic/osteogenic differentiation with calcium hydroxide. Methods: Primary pulp cells were harvested from the molars of C57BL/6 mice. The cells were treated with calcium hydroxide. Immunofluorescence was used to detect protein expression. A knockout of the ephrinB1 or EphB2 gene was performed with short hairpin RNAs. Cell migration, proliferation, and gene expression were then analyzed. Results: Calcium hydroxide stimulated EphB2 gene expression but suppressed ephrinB1 gene expression at the proliferation stage. However, calcium hydroxide stimulated both ephrinB1 and EphB2 gene expression at the differentiation stage. In addition, EphB2 localized at ephrinB1-positive cells at the area of Dentin sialoprotein (DSP) staining, which increased with calcium hydroxide treatment. Knockdown of ephrinB1-EphB2 significantly suppressed cell proliferation. Additionally, knockdown of the ephrinB1 gene caused cell migration, whereas a lack of the EphB2 gene suppressed calcium hydroxide-induced mineralization from primary pulp cells. Conclusions: EphrinB1-EphB2 interaction contributes to calcium hydroxide induced odontogenic/osteogenic differentiation. This observation is the first finding of the mechanism of calcium hydroxide-induced odontogenic/osteogenic differentiation.

语种: 英语
项目资助者: American Association of Endodontists Foundation Research Grant
WOS记录号: WOS:000325448100008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59425
Appears in Collections:北京大学口腔医学院_口腔预防保健科_期刊论文

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作者单位: 1.NYU, Coll Dent, Dept Basic Sci & Craniofacial Biol, New York, NY 10010 USA
2.NYU, Coll Dent, Dept Endodont, New York, NY 10010 USA
3.Peking Univ, Sch & Hosp Stomatol, Dept Prevent Dent, Beijing 100871, Peoples R China

Recommended Citation:
Wang, Xiaozhe,Jong, George,Lin, Louis M.,et al. EphB-EphrinB Interaction Controls Odontogenic/Osteogenic Differentiation with Calcium Hydroxide[J]. JOURNAL OF ENDODONTICS,2013,39(10):1256-1260.
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