IR@PKUHSC  > 北京大学第一临床医学院  > 血液内科
学科主题临床医学
Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm
Hou, Yong1,2,3; Song, Luting1,4,5,6; Zhu, Ping7; Zhang, Bo1; Tao, Ye1; Xu, Xun1; Li, Fuqiang1; Wu, Kui1; Liang, Jie1; Shao, Di1; Wu, Hanjie1; Ye, Xiaofei1; Ye, Chen1; Wu, Renhua1; Jian, Min1; Chen, Yan; Xie, Wei1,3; Zhang, Ruren1,3; Chen, Lei1,4,5,6; Liu, Xin1; Yao, Xiaotian1; Zheng, Hancheng1; Yu, Chang1; Li, Qibin1; Gong, Zhuolin1; Mao, Mao8; Yang, Xu1; Yang, Lin1; Li, Jingxiang1; Wang, Wen5; Lu, Zuhong2,3; Gu, Ning2,3; Laurie, Goodman1; Bolund, Lars1; Kristiansen, Karsten1,9; Wang, Jian1; Yang, Huanming1; Li, Yingrui1; Zhang, Xiuqing1; Wang, Jun1,9,10
刊名CELL
2012-03-02
DOI10.1016/j.cell.2012.02.028
148期:5页:873-885
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
资助者National Basic Research Program of China (973 program) ; Chinese 863 program ; Shenzhen Municipal Government of China ; Key Laboratory Project, Shenzhen City ; Shenzhen Key Laboratory of Gene Bank for National Life Science ; Innovative Research Team of Guangdong ; Guangdong Enterprise Key Laboratory of Human Disease Genomics ; Danish Natural Science Research Council ; Danish National Research Foundation ; National Natural Science Foundation of China ; Shenzhen Municipal Government ; Local Government of Yantian District of Shenzhen ; National Basic Research Program of China (973 program) ; Chinese 863 program ; Shenzhen Municipal Government of China ; Key Laboratory Project, Shenzhen City ; Shenzhen Key Laboratory of Gene Bank for National Life Science ; Innovative Research Team of Guangdong ; Guangdong Enterprise Key Laboratory of Human Disease Genomics ; Danish Natural Science Research Council ; Danish National Research Foundation ; National Natural Science Foundation of China ; Shenzhen Municipal Government ; Local Government of Yantian District of Shenzhen
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]MULTIPLE DISPLACEMENT AMPLIFICATION ; ESSENTIAL THROMBOCYTHEMIA ; CANCER ; MUTATIONS ; VARIANTS ; ORIGIN ; GENES
英文摘要

Tumor heterogeneity presents a challenge for inferring clonal evolution and driver gene identification. Here, we describe a method for analyzing the cancer genome at a single-cell nucleotide level. To perform our analyses, we first devised and validated a high-throughput whole-genome single-cell sequencing method using two lymphoblastoid cell line single cells. We then carried out whole-exome single-cell sequencing of 90 cells from a JAK2-negative myeloproliferative neoplasm patient. The sequencing data from 58 cells passed our quality control criteria, and these data indicated that this neoplasm represented a monoclonal evolution. We further identified essential thrombocythemia (ET)-related candidate mutations such as SESN2 and NTRK1, which may be involved in neoplasm progression. This pilot study allowed the initial characterization of the disease-related genetic architecture at the single-cell nucleotide level. Further, we established a single-cell sequencing method that opens the way for detailed analyses of a variety of tumor types, including those with high genetic complex between patients.

语种英语
所属项目编号2011CB809202 ; 2011CB809203 ; 2009AA022707 ; 2012AA02A201 ; ZYC201005250020A ; CX B200903110066A ; CXB201108250096A
资助者National Basic Research Program of China (973 program) ; Chinese 863 program ; Shenzhen Municipal Government of China ; Key Laboratory Project, Shenzhen City ; Shenzhen Key Laboratory of Gene Bank for National Life Science ; Innovative Research Team of Guangdong ; Guangdong Enterprise Key Laboratory of Human Disease Genomics ; Danish Natural Science Research Council ; Danish National Research Foundation ; National Natural Science Foundation of China ; Shenzhen Municipal Government ; Local Government of Yantian District of Shenzhen ; National Basic Research Program of China (973 program) ; Chinese 863 program ; Shenzhen Municipal Government of China ; Key Laboratory Project, Shenzhen City ; Shenzhen Key Laboratory of Gene Bank for National Life Science ; Innovative Research Team of Guangdong ; Guangdong Enterprise Key Laboratory of Human Disease Genomics ; Danish Natural Science Research Council ; Danish National Research Foundation ; National Natural Science Foundation of China ; Shenzhen Municipal Government ; Local Government of Yantian District of Shenzhen
WOS记录号WOS:000300985000009
引用统计
被引频次:263[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59427
专题北京大学第一临床医学院_血液内科
作者单位1.BGI Shenzhen, Shenzhen 518083, Peoples R China
2.Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
3.Southeast Univ, Sch Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China
4.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
5.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, CAS Max Planck Jr Res Grp, Kunming 650223, Yunnan, Peoples R China
6.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
7.Peking Univ, Hosp 1, Dept Hematol, Beijing 100034, Peoples R China
8.Pfizer Inc, San Diego, CA 92121 USA
9.Univ Copenhagen, Dept Biol, DK-1165 Copenhagen, Denmark
10.Univ Copenhagen, Novo Nordisk Fdn Ctr Basic Metab Res, DK-1165 Copenhagen, Denmark
推荐引用方式
GB/T 7714
Hou, Yong,Song, Luting,Zhu, Ping,et al. Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm[J]. CELL,2012,148(5):873-885.
APA Hou, Yong.,Song, Luting.,Zhu, Ping.,Zhang, Bo.,Tao, Ye.,...&Wang, Jun.(2012).Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm.CELL,148(5),873-885.
MLA Hou, Yong,et al."Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm".CELL 148.5(2012):873-885.
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