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学科主题: 临床医学
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Self-aggregated deglycosylated IgA1 with or without IgG were associated with the development of IgA nephropathy
作者: Yan, Y; Xu, LX; Zhang, JJ; Zhang, Y; Zhao, MH
关键词: deglycosylation ; IgA nephropathy ; macromolecular IgA1 ; pathology
刊名: CLINICAL AND EXPERIMENTAL IMMUNOLOGY
发表日期: 2006-04-01
DOI: 10.1111/j.1365-2249.2006.03026.x
卷: 144, 期:1, 页:17-24
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: HUMAN MESANGIAL CELLS ; IMMUNE-COMPLEXES ; GLOMERULAR DEPOSITION ; O-GLYCOSYLATION ; HUMAN SERUM ; BINDING ; IMMUNOGLOBULINS
英文摘要:

IgA nephropathy (IgAN) is the most common primary glomerulonephritis, with various pathological phenotypes. Our previous study suggested that aberrant glycosylation of serum IgA1 was associated with different pathological phenotypes of IgAN, and substantial evidence indicated that deglycosylated IgA1 had an increased tendency to form macromolecules. The aim of the current study was to investigate the composition of IgA1-containing macromolecules in different pathological phenotypes of IgAN. Sera from 10 patients with mild mesangial proliferative IgAN (mIgAN), 10 with focal proliferative sclerosing IgAN (psIgAN) and 10 healthy blood donors were collected. The sera were applied and IgA1 binding proteins (IgA1-BP) were eluted from the columns immobilized with desialylated IgA1 (DesIgA1/Sepharose) or desialylated/degalactosylated IgA1 (DesDeGalIgA1/Sepharose), respectively. The amounts of IgA1 and IgG and the glycoform of IgA1 in the IgA1-BP were detected by enzyme-linked immunosorbent assays (ELISAs) and were compared between patients with different pathological phenotypes and normal controls. The amount of IgA1 in IgA1-BP eluted from both columns was significantly higher in patients with both pathological phenotypes of IgAN than in normal controls. In IgA1-BP eluted from DesDeGalIgA1/Sepharose, the desialylation of IgA1 was much more pronounced in patients with both pathological phenotypes of IgAN than in normal controls, while the degalactosylation of IgA1 was much more pronounced only in patients with psIgAN than in normal controls. Furthermore, the amount of IgG in IgA1-BP eluted from DesDeGalIgA1/Sepharose was significantly higher in patients with psIgAN than in normal controls. In patients with psIgAN, the amount of IgG eluted from DesDeGalIgA1/Sepharose was much greater than from DesIgA1/Sepharose. In conclusion, self-aggregated deglycosylated IgA1 with or without IgG were associated with the development of IgAN.

语种: 英语
WOS记录号: WOS:000236022500003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59428
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: 1.Peking Univ First Hosp, Div Renal, Inst Renal Div, Beijing 100034, Peoples R China
2.Peking Univ First Hosp, Inst Nephrol, Beijing 100034, Peoples R China

Recommended Citation:
Yan, Y,Xu, LX,Zhang, JJ,et al. Self-aggregated deglycosylated IgA1 with or without IgG were associated with the development of IgA nephropathy[J]. CLINICAL AND EXPERIMENTAL IMMUNOLOGY,2006,144(1):17-24.
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