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学科主题: 临床医学
题名:
Isoflurane anesthesia results in reversible ultrastructure and occludin tight junction protein expression changes in hippocampal blood-brain barrier in aged rats
作者: Cao, Yiyun1; Ni, Cheng1; Li, Zhengqian1; Li, Lunxu1; Liu, Yajie1; Wang, Chunyi1; Zhong, Yanfeng2; Cui, Dehua3; Guo, Xiangyang1
关键词: Blood-brain barrier ; Tight junction protein ; Hippocampus ; Postoperative cognitive dysfunction
刊名: NEUROSCIENCE LETTERS
发表日期: 2015-02-05
DOI: 10.1016/j.neulet.2014.12.018
卷: 587, 页:51-56
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: COGNITIVE DYSFUNCTION ; PERMEABILITY ; BETA ; DISRUPTION ; NEUROINFLAMMATION ; DESFLURANE ; ACTIVATION ; PATHWAY ; SURGERY ; CELLS
英文摘要:

The underlying mechanism of isoflurane-induced cognitive dysfunction in older individuals is unknown. In this study, the effects of isoflurane exposure on the hippocampal blood brain barrier (BBB) in aged rats were investigated because it was previously shown that BBB disruption involves in cognitive dysfunction. Twenty-month-old rats randomly received 1.5% isoflurane or vehicle gas as control. Hippocampal BBB ultrastructure was analyzed by transmission electron microscopy and expression of tight junction proteins was measured by western blot analysis. BBB permeability was detected with sodium fluorescein extravasation and further confirmed by immunoglobulin G immunohistochemistry. Spatial learning and memory were assessed by the Morris water maze test. Isoflurane anesthesia resulted in reversible time-dependent BBB ultrastructure morphological damage and significant decreases in expression of the tight junction proteins occludin, which contributed to sodium fluorescein and IgG leakage. Rats with isoflurane exposure also showed significant cognitive deficits in the Morris water maze test. This in vivo data indicate that occludin down-regulation may be one of the mediators of isoflurane-induced hippocampus BBB disruption, and may contribute to hippocampus-dependent cognitive impairment after isoflurane exposure in aged rats. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 81371205 ; 2012CB911004 ; 20110001110008
项目资助者: National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Doctoral Fund of the Ministry of Education of China
WOS记录号: WOS:000349061900011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59436
Appears in Collections:北京大学第三临床医学院_麻醉科_期刊论文

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作者单位: 1.Peking Univ, Dept Anesthesiol, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Pathol, Beijing 100191, Peoples R China
3.Peking Univ, Neurosci Res Inst, Dept Neurobiol, Beijing 100191, Peoples R China

Recommended Citation:
Cao, Yiyun,Ni, Cheng,Li, Zhengqian,et al. Isoflurane anesthesia results in reversible ultrastructure and occludin tight junction protein expression changes in hippocampal blood-brain barrier in aged rats[J]. NEUROSCIENCE LETTERS,2015,587:51-56.
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