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Isoflurane anesthesia results in reversible ultrastructure and occludin tight junction protein expression changes in hippocampal blood-brain barrier in aged rats
Cao, Yiyun1; Ni, Cheng1; Li, Zhengqian1; Li, Lunxu1; Liu, Yajie1; Wang, Chunyi1; Zhong, Yanfeng2; Cui, Dehua3; Guo, Xiangyang1
关键词Blood-brain barrier Tight junction protein Hippocampus Postoperative cognitive dysfunction
刊名NEUROSCIENCE LETTERS
2015-02-05
DOI10.1016/j.neulet.2014.12.018
587页:51-56
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]COGNITIVE DYSFUNCTION ; PERMEABILITY ; BETA ; DISRUPTION ; NEUROINFLAMMATION ; DESFLURANE ; ACTIVATION ; PATHWAY ; SURGERY ; CELLS
英文摘要

The underlying mechanism of isoflurane-induced cognitive dysfunction in older individuals is unknown. In this study, the effects of isoflurane exposure on the hippocampal blood brain barrier (BBB) in aged rats were investigated because it was previously shown that BBB disruption involves in cognitive dysfunction. Twenty-month-old rats randomly received 1.5% isoflurane or vehicle gas as control. Hippocampal BBB ultrastructure was analyzed by transmission electron microscopy and expression of tight junction proteins was measured by western blot analysis. BBB permeability was detected with sodium fluorescein extravasation and further confirmed by immunoglobulin G immunohistochemistry. Spatial learning and memory were assessed by the Morris water maze test. Isoflurane anesthesia resulted in reversible time-dependent BBB ultrastructure morphological damage and significant decreases in expression of the tight junction proteins occludin, which contributed to sodium fluorescein and IgG leakage. Rats with isoflurane exposure also showed significant cognitive deficits in the Morris water maze test. This in vivo data indicate that occludin down-regulation may be one of the mediators of isoflurane-induced hippocampus BBB disruption, and may contribute to hippocampus-dependent cognitive impairment after isoflurane exposure in aged rats. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000349061900011
项目编号81371205 ; 2012CB911004 ; 20110001110008
资助机构National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Doctoral Fund of the Ministry of Education of China
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59436
专题北京大学第三临床医学院_麻醉科
北京大学基础医学院
作者单位1.Peking Univ, Dept Anesthesiol, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Pathol, Beijing 100191, Peoples R China
3.Peking Univ, Neurosci Res Inst, Dept Neurobiol, Beijing 100191, Peoples R China
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GB/T 7714
Cao, Yiyun,Ni, Cheng,Li, Zhengqian,et al. Isoflurane anesthesia results in reversible ultrastructure and occludin tight junction protein expression changes in hippocampal blood-brain barrier in aged rats[J]. NEUROSCIENCE LETTERS,2015,587:51-56.
APA Cao, Yiyun.,Ni, Cheng.,Li, Zhengqian.,Li, Lunxu.,Liu, Yajie.,...&Guo, Xiangyang.(2015).Isoflurane anesthesia results in reversible ultrastructure and occludin tight junction protein expression changes in hippocampal blood-brain barrier in aged rats.NEUROSCIENCE LETTERS,587,51-56.
MLA Cao, Yiyun,et al."Isoflurane anesthesia results in reversible ultrastructure and occludin tight junction protein expression changes in hippocampal blood-brain barrier in aged rats".NEUROSCIENCE LETTERS 587(2015):51-56.
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