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Successful reprogramming of differentiated cells by somatic cell nuclear transfer, using in vitro-matured oocytes with a modified activation method
Yu, Yang1,3; Yan, Jie1,3; Zhang, Qiufang1,3; Yan, Liying1,3; Li, Min1,3; Zhou, Qi2; Qiao, Jie1,3
关键词somatic cell nuclear transfer reprogramming therapeutic cloning activation ionomycin 6-dimethylaminopurine pronuclear morphology
刊名JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
2013-11-01
DOI10.1002/term.1476
7期:11页:855-863
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology ; Engineering, Biomedical
资助者Ministry of Science and Technology of China (973 program) ; National Natural Science Funds for Distinguished Young Scholars ; Ministry of Science and Technology of China (973 program) ; National Natural Science Funds for Distinguished Young Scholars
研究领域[WOS]Cell Biology ; Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]EMBRYONIC STEM-CELLS ; 6-DIMETHYLAMINOPURINE 6-DMAP ; DEVELOPMENTAL COMPETENCE ; BOVINE OOCYTES ; MOUSE OOCYTES ; INTERPHASE ; BLASTOCYST ; IONOPHORE ; IONOMYCIN ; FIBROBLASTS
英文摘要

Therapeutic cloning has tremendous potential for cell therapy and tissue repair in some diseases. However, the efficiency of development of cloned human embryos by somatic cell nuclear transfer is still low. In the present study, the activation of cloned human embryos was investigated while using in vitro-matured oocytes. Pseudo-pronuclear formation and the subsequent development was compared with different activation parameters, including different durations of ionomycin and 6-dimethylaminopurine treatment. The results showed that somatic cells were successfully reprogrammed by modification of activation treatments while using in vitro-matured oocytes. The activation efficiency of cloned human embryos was significantly increased at durations of ionomycin at both 5 and 7min, despite different durations of 6-DMAP treatment. The results of blastocyst development showed that 20% of activated embryos developed to the blastocyst stage when the embryos were activated with 5 mu m ionomycin for 5min and 2mm 6-DMAP for 5h, which was significantly higher than those activated with other parameters. Moreover, we found that an increasing duration of 6-DMAP induced the formation of a single, large, pseudo-pronucleus in cloned human embryos and impaired subsequent development competence. In conclusion, successful reprogramming of human somatic cells was achieved using in vitro-matured oocytes by somatic cell nuclear transfer and improved with a modified activation method. Copyright (c) 2012 John Wiley & Sons, Ltd.

语种英语
所属项目编号2011CB944504 ; 31000661
资助者Ministry of Science and Technology of China (973 program) ; National Natural Science Funds for Distinguished Young Scholars ; Ministry of Science and Technology of China (973 program) ; National Natural Science Funds for Distinguished Young Scholars
WOS记录号WOS:000326418400002
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59438
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
3.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
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GB/T 7714
Yu, Yang,Yan, Jie,Zhang, Qiufang,et al. Successful reprogramming of differentiated cells by somatic cell nuclear transfer, using in vitro-matured oocytes with a modified activation method[J]. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,2013,7(11):855-863.
APA Yu, Yang.,Yan, Jie.,Zhang, Qiufang.,Yan, Liying.,Li, Min.,...&Qiao, Jie.(2013).Successful reprogramming of differentiated cells by somatic cell nuclear transfer, using in vitro-matured oocytes with a modified activation method.JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,7(11),855-863.
MLA Yu, Yang,et al."Successful reprogramming of differentiated cells by somatic cell nuclear transfer, using in vitro-matured oocytes with a modified activation method".JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 7.11(2013):855-863.
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